Abstract
Hydration of bone tissue and products of its thermal and chemical degradation in the presence of organic solvents was studied using low-temperature 1H NMR spectroscopy and cryoporometry. It was revealed that water filling nanosized cavities between structural elements of bone tissue can be assigned to two types of clustered structures corresponding to strongly and weakly associated water reflecting in the 1H NMR spectra as separate signals with chemical shift δ H = 1.3 and 5 ppm. It was shown that the chloroform medium stabilizes weakly associated water and reduces interaction of strongly associated water with the phase boundaries. The same tendency is outlined in protein and mineral components of bovine bone. The presence of electron-donor solvents (acetonitrile, DMSO) leads to formation of clustered structures (with electron-donor molecules, strongly associated water or water solution in organic solvents) in the hydrate shells of structural elements of bone materials. In contrast to the native bone material, individual model systems with proteins or mineral components are characterized by a considerable diminution or complete disappearance of weakly associated water.
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Turov, V. et al. (2009). Self-Organization of Water–Organic Systems in Bone Tissue and Products of Its Chemical Degradation. In: Shpak, A., Gorbyk, P. (eds) Nanomaterials and Supramolecular Structures. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-2309-4_7
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DOI: https://doi.org/10.1007/978-90-481-2309-4_7
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