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Vitrification Solutions: Historical Development

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Vitrification in Assisted Reproduction

Abstract

Fertility is one of the medical areas having a very rapid evolution. This chapter is about the history and evolution of cryoprotectants and vitrification. The beginnings of cryopreservation, dating from the early eighteenth century to date, are described in detail. The history of cryopreservation has evolved from the plant cells in the beginning to animals, vitrified germ cells, embryos, oocytes, and to even other tissues to be used for transplantation.

This process has not been easy. It is not enough to know that a cell can survive after being frozen to −286 °C. It is important to ensure that the least possible damage is done to the cell during this process. This chapter offers a revision from the first steps of cryopreservation to what we know today as the ultrarapid vitrification method, which lasts a few seconds, achieving rates of freezing up to −23,000 °C/min using permeating and nonpermeating cryoprotectants, in order to avoid damage to the cell structure by the formation of ice crystals within. This chapter mentions the latest technologies and techniques available for the vitrification nowadays, as well as the objectives and forward-looking in vitrification area.

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References

  1. Kuleshova LL, Lopata Alex MB. Vitrification can be more favorable tan slow cooling. Fertil Steril. 2002;78(3):449–54.

    Article  PubMed  Google Scholar 

  2. Leibo S, Pool TB. The principal variables of cryopreservation: solutions, temperatures and change rates. Fertil Steril. 2011;96(2):269–76.

    Article  PubMed  CAS  Google Scholar 

  3. Liebermann J. Chapter 6. Vitrification of oocytes and embryos. In: Current frontiers in criobiology. Chicago: Fertility Centers of Illinois; 2012. p. 169–84.

    Google Scholar 

  4. Fuller B. Cryoprotectants: the essential antifreezes to protect life in the frozen state. Cryo Letters. 2004;25(6):375–88.

    PubMed  CAS  Google Scholar 

  5. Maximov NA. Berichte der Deutschen botanischen Gesellschaft. 1912;30:52–65.

    Google Scholar 

  6. Cobo A, Garcia-Velasco JA, Domingo J, Remohi J, Pellicer A. Is vitrification of oocytes useful for fertility preservation for age-related fertility decline and in cancer patients? Fertil Steril. 2013;99(6):1485–95.

    Article  PubMed  Google Scholar 

  7. Chang MC. Transplantation of fertilized rabbit ova: the effect on viability of age, in vitro storage period & storage temperature. Nature. 1948;161:978.

    Article  PubMed  CAS  Google Scholar 

  8. Polge C, Smith AU, Parkes AS. Revival of spermatozoa after vitrification & dehydration at low temperatures. Nature. 1949;164:666.

    Article  PubMed  CAS  Google Scholar 

  9. Wennerholm UB. Cryopreservation of embryos and oocytes: obstetric outcome and health in children. Hum Reprod. 2000;15 Suppl 5:18–25.

    Article  PubMed  Google Scholar 

  10. Ludwig M, Al-Hasani S, Ferlberbaum R, Diedrich K. New aspects of cryopreservation of oocytes ad embryos in assisted reproduction and future perspectives. Hum Reprod. 1999;14 Suppl 1:162–85.

    Article  PubMed  Google Scholar 

  11. Garcia JI, Noriega-Portella L, Noriega-Hoces L. Efficacy of oocyte vitrification combined with blastocyst stage transfer in an egg donation program. Hum Reprod. 2011;26(4):782–90.

    Article  PubMed  Google Scholar 

  12. Kuwayama M, Vajta G, Ieda S, Kato O. Comparison of open and closed methods for vitrification of human embryos and the elimination of potential contamination. Kato Ladies Clinic. Tokyo Japan. Reprod Biomed Online. 2005;11(5). [Internet]. Available from: www.rbmonline.com/Article/1925. Accessed Sept 2005.

  13. Jain JK, Paulson RJ. Oocyte cryopreservation. Fertil Steril. 2006;86 Suppl 3:1037–46.

    Article  PubMed  CAS  Google Scholar 

  14. Courtney BS, Lane M, Gardner DK. The Cryoloop facilitates re-vitrification of embryos at four successive stages of development without impairing embryo growth. Hum Reprod. 2006;21(11):2978–84.

    Article  Google Scholar 

  15. Chen SU, Lien YR, Chen Y, Chen HF, Ho HN, Yang Y. Vitrification of mouse oocytes using closed pulled straws (CPS) achieves a high survival and preserves good patterns of meiotic spindles, compared with conventional straws, open pulled straws (OPS) and grids. Hum Reprod. 2001;16(11):2350–6.

    PubMed  CAS  Google Scholar 

  16. Chian RC, Wang Y, Li Y. Oocyte vitrification: advances, progress and future goals. Technological innovations. J Assist Reprod Genet. 2014;31(4):411–20.

    Article  PubMed  Google Scholar 

  17. Mukaida T, Nakamura S, Tomiyama T, Wada S, Kasai M, Takahashi K. Successful birth after transfer of vitrified human blastocysts with use of a cryoloop containerless technique. Fertil Steril. 2001;76(3):618–20.

    Article  PubMed  CAS  Google Scholar 

  18. Criado SE. Chapter 6. The problem of contamination: open vs. closed vs semi-closed vitrification system. In: Current frontiers in cryopreservation. Marbella: CERAM: Centre of Assisted Reproduction; 2012. p. 105–36.

    Google Scholar 

  19. Keros V, Xella S, Hultenby K, Pettersson K, Sheikhi M, Volpe A, et al. Vitrification versus controlled-rate freezing in cryopreservation of human ovarian tissue. Hum Reprod. 2009;24(7):1670–83.

    Article  PubMed  CAS  Google Scholar 

  20. Kuwayama M. Highly efficient vitrification for cryopreservation of human oocytes and embryos: the Cryotop method. Theriogenology. 2007;67(1):73–80.

    Article  PubMed  CAS  Google Scholar 

  21. Chang CC. Method for microdrop vitrification of cells. Livestock Research Institute; 2006.

    Google Scholar 

  22. Xing W, Zhou C, Bian J, Montag M, Xu Y, Li Y, et al. Solid surface vitrification is an appropriate and convenient method for cryopreservation of isolated rat follicles. Reprod Biol Endocrinol. 2010;8:42. doi:10.1186/1477-7827-8-42.

    Article  PubMed  PubMed Central  Google Scholar 

  23. Gandhi G, Allahbadia G, Kagalwala S, Allahbadia A, Ramesh S, Patel K, et al. ACCU-VIT: a new strategy for managing poor responders. London: e-poster ESHRE; 2013.

    Google Scholar 

  24. Kagalwala S, Gandhi G, Allahbadia G, Kuwayama M, Allahbadia A, Chipkar V, et al. A comparison of two different vitrification methods for cryopreservation of mature human oocytes. London: e-poster ESHRE; 2013.

    Google Scholar 

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Correspondence to Luis Arturo Ruvalcaba Castellon MD .

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Castellon, L.A.R., Loya, A.G.C. (2015). Vitrification Solutions: Historical Development. In: Allahbadia, G., Kuwayama, M., Gandhi, G. (eds) Vitrification in Assisted Reproduction. Springer, New Delhi. https://doi.org/10.1007/978-81-322-1527-1_2

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  • DOI: https://doi.org/10.1007/978-81-322-1527-1_2

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  • Publisher Name: Springer, New Delhi

  • Print ISBN: 978-81-322-1526-4

  • Online ISBN: 978-81-322-1527-1

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