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Fish-Processing Industrial Effluent Characterization and Partitioning of Proteins Using Aqueous Two-Phase System

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Prospects in Bioscience: Addressing the Issues

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Abstract

Characterization of fish-processing industrial effluent was carried out according to Bureau of Indian Standards (BS.2690:1965). The effluent contained high strength of chemical oxygen demand, biological oxygen demand and proteins. The effect of protein solubility was measured by a function of pH ranging from 2 to 12. Partitioning of proteins from fish industrial effluent in aqueous two-phase systems (ATPS) was investigated. Sodium sulphate was chosen due to its ability of promoting the hydrophobic difference between the phases. Phase compositions including polyethylene glycol (PEG) molecular weight (2000, 4000, 6000 and 8000) and sodium sulphate affecting the protein partitioning were studied. Varying the polyethylene glycol molecular weight and concentrations with sodium sulphate salt affects the partition coefficient. The tie lines for above-said system were developed at 25°C for different compositions. Further, tie-line lengths were calculated and effects of this parameter over partition coefficient were studied. From the experimental results, it was revealed that the PEG molecular weight and concentrations, salt concentrations, pH (5–8) and temperature (20–50°C) of system had strong impacts on partition features.

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Acknowledgement

The authors acknowledge a grant (Scheme No. 01(2339)/09/EMR-II) from the Council of Scientific and Industrial Research (CSIR), Government of India, for this research.

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Authors and Affiliations

  1. Department of Chemical Engineering, National Institute of Technology – Karnataka, Srinivasnagar Post, Surathkal, Mangalore, Karnataka, 575025, India

    H. Nagaraja Viswanatha & I. Regupathi

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  1. H. Nagaraja Viswanatha
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  2. I. Regupathi
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Correspondence to H. Nagaraja Viswanatha .

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Editors and Affiliations

  1. , Dept of Biotechnology & Microbiology, Kannur University, Palayad P.O,Thalassery Campus, Kannur, 670661, Kerala, India

    Abdulhameed Sabu

  2. , Dept. of Biotechnology and Microbiology, Kannur University, Palayad P.O, Thalassery Campus, Kannur, 670661, Kerala, India

    Anu Augustine

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Viswanatha, H.N., Regupathi, I. (2012). Fish-Processing Industrial Effluent Characterization and Partitioning of Proteins Using Aqueous Two-Phase System. In: Sabu, A., Augustine, A. (eds) Prospects in Bioscience: Addressing the Issues. Springer, India. https://doi.org/10.1007/978-81-322-0810-5_1

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