Abstract
The oral gingiva, which is often resected during dental treatments, is an easily obtainable tissue, and cells can be isolated from patients with minimal discomfort. We generated induced pluripotent stem (iPS) cells from adult mouse or human gingival fibroblasts (GFs) using retroviral transduction of the Yamanaka factors (Egusa et al., PLoS One, 2010). These iPS cells exhibited characteristics similar to embryonic stem cells. The reprogramming efficiency of mouse GFs was higher than that of tail skin fibroblasts. Fibroblasts from gingival tissues that are easily obtained by dentists can be readily reprogrammed into iPS cells, thus making them a promising cell source for future oral tissue engineering applications, as well as for in vitro applications for drug screening and the generation of disease-specific iPS cells for tailor-made diagnostics. Current hurdles that will need to be cleared if iPS cells are to fulfill their clinical promise are also outlined in this mini-review.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Egusa H, Saeki M, Doi M et al (2010) A small-molecule approach to bone regenerative medicine in dentistry. J Oral Biosci 52:107–118
Kaigler D, Mooney D (2001) Tissue engineering’s impact on dentistry. J Dent Educ 65:456–462
Estrela C, Alencar AH, Kitten GT et al (2011) Mesenchymal stem cells in the dental tissues: perspectives for tissue regeneration. Braz Dent J 22:91–98
Takahashi K, Yamanaka S (2006) Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126:663–676
Takahashi K, Tanabe K, Ohnuki M et al (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131:861–872
Aoi T, Yae K, Nakagawa M et al (2008) Generation of pluripotent stem cells from adult mouse liver and stomach cells. Science 321:699–702
Seki T, Yuasa S, Oda M et al (2010) Generation of induced pluripotent stem cells from human terminally differentiated circulating T cells. Cell Stem Cell 7:11–14
Kim JB, Zaehres H, Wu G et al (2008) Pluripotent stem cells induced from adult neural stem cells by reprogramming with two factors. Nature 454:646–650
Sun N, Panetta NJ, Gupta DM et al (2009) Feeder-free derivation of induced pluripotent stem cells from adult human adipose stem cells. Proc Natl Acad Sci USA 106:15720–15725
Walia B, Satija N, Tripathi RP et al (2011) Induced pluripotent stem cells: fundamentals and applications of the reprogramming process and its ramifications on regenerative medicine. Stem Cell Rev. doi:10.1007/s12015-011-9279-x
Yu J, Vodyanik MA, Smuga-Otto K et al (2007) Induced pluripotent stem cell lines derived from human somatic cells. Science 318:1917–1920
Stephens P, Davies KJ, Occleston N et al (2001) Skin and oral fibroblasts exhibit phenotypic differences in extracellular matrix reorganization and matrix metalloproteinase activity. Br J Dermatol 144:229–237
Sukotjo C, Lin A, Song K et al (2003) Oral fibroblast expression of wound-inducible transcript 3.0 (wit3.0) accelerates the collagen gel contraction in vitro. J Biol Chem 278:51527–51534
Sciubba JJ, Waterhouse JP, Meyer J (1978) A fine structural comparison of the healing of incisional wounds of mucosa and skin. J Oral Pathol 7:214–227
Walsh LJ, L’Estrange PR, Seymour GJ (1996) High magnification in situ viewing of wound healing in oral mucosa. Aust Dent J 41:75–79
Giannopoulou C, Cimasoni G (1996) Functional characteristics of gingival and periodontal ligament fibroblasts. J Dent Res 75:895–902
Egusa H, Okita K, Kayashima H et al (2010) Gingival fibroblasts as a promising source of induced pluripotent stem cells. PLoS One 5:e12743
Duan X, Tu Q, Zhang J et al (2011) Application of induced pluripotent stem (iPS) cells in periodontal tissue regeneration. J Cell Physiol 226:150–157
Cho HJ, Lee CS, Kwon YW et al (2010) Induction of pluripotent stem cells from adult somatic cells by protein-based reprogramming without genetic manipulation. Blood 116:386–395
Okita K, Matsumura Y, Sato Y et al (2011) A more efficient method to generate integration-free human iPS cells. Nat Methods 8:409–412
Takahashi K, Narita M, Yokura M et al (2009) Human induced pluripotent stem cells on autologous feeders. PLoS One 4:e8067
Hayashi Y, Chan T, Warashina M et al (2010) Reduction of N-glycolylneuraminic acid in human induced pluripotent stem cells generated or cultured under feeder- and serum-free defined conditions. PLoS One 5:e14099
Zhao T, Zhang ZN, Rong Z et al (2011) Immunogenicity of induced pluripotent stem cells. Nature 474:212–215
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer
About this paper
Cite this paper
Egusa, H. (2012). The Impact of Gingival Fibroblast-Derived iPS Cells in Dentistry. In: Sasaki, K., Suzuki, O., Takahashi, N. (eds) Interface Oral Health Science 2011. Springer, Tokyo. https://doi.org/10.1007/978-4-431-54070-0_2
Download citation
DOI: https://doi.org/10.1007/978-4-431-54070-0_2
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-54069-4
Online ISBN: 978-4-431-54070-0
eBook Packages: MedicineMedicine (R0)