Zusammenfassung
In seltenen Fällen werden biologische Proben direkt, ohne weitere Bearbeitung, im Licht- oder gar im Elektronenmikroskop betrachtet. Die meisten Fragestellungen und die meisten Objekte erfordern eine mehr oder weniger umfangreiche Präparation. Das folgende, ebenfalls recht umfangreiche Kapitel gibt einen Einstieg in die vielfältigen Möglichkeiten, mikroskopische Präparate für licht- und elektronenmikroskopische Untersuchungen herzustellen. Die detaillierten Anleitungen stehen beispielhaft für ähnliche Untersuchungsziele und -objekte. Mit Hilfe der Erklärungen im Text können sie für eigene Fragestellungen abgewandelt, ergänzt oder miteinander kombiniert werden.
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2.8 Literatur
Literatur Kapitel 2.1.1–2.1.3
Axelrod D, Koppel DE, Schlessinger J, Elson E, Webb WW (1976) Mobility measurement by analysis of fluorescence photobleaching recovery kinetics. Biophys J 16:1055–1069
Bastiaens PI, Squire A (1999) Fluorescence lifetime imaging microscopy: spatial resolution of biochemical processes in the cell. Trends Cell Biol 9(2):48–52
Bates IR, Wiseman PM, Hanrahan JW (2006) Investigating membrane protein dynamics in living cells. Biochem Cell Biol 84(6):825–31
Choi HS, Kim JW, Cha YN, Kim C (2006) A quantitative nitroblue tetrazolium assay for determining intracellular superoxide anion production in phagocytic cells. J Immunoassay Immunochem 27(1):31–44
Dahl LK (1952) A simple and sensitive histochemical method for calcium. Proc Soc exp Biol Med 80:474–479
Day RN, Schaufele F (2005) Imaging molecular interactions in living cells. Mol Endocrinol 19(7):1675–1686
Doan CA, Ralph P (1950) In: Handbook of microscopical Technique. McClung CE (ed), Paul B Hoeber, New York, NY
Ficz G, Heintzmann R, Arndt-Jovin DJ (2005) Polycomb group protein complexes exchange rapidly in living Drosophila. Development 132(17):3963–3976.
Fricker M, Runions J, Moore I (2006) Quantitative Fluorescence Microscopy: From Art to Science. Annu Rev Plant Biol 2006.57:79–107
Gennerich A (2003) Fluoreszenskorrelationsspektroskopie und Rasterkorrelationsmikroskopie molekularer Prozesse in Nervenzellen. Dissertation, Georg-August-Universität zu Göttingen, Göttingen. http://webdoc.sub. gwdg.de/diss/2003/gennerich/gennerich.pdf
Gilbert D (2006) Chlorid-basierte Signalverstärkung in Capsaicin-sensitiven Schmerzzellen. Dissertation, Ruprecht-Karls-Universität Heidelberg. http://www.ub.uni-heidelberg.de/archiv/6711/
Goldman RD, Spector DL (2005) Live cell imaging: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
Hebert TE, Gales C, Rebois RV (2006) Detecting and imaging protein-protein interactions during G protein-mediated signal transduction in vivo and in situ by using fluorescence-based techniques. Cell Biochem Biophys 45(1):85–109. Review
Hoebe RA, Van Oven CH, Gadella TWJ, Dhonukshe Jr. PB, Van Noorden CJF, Manders EMM (2007) Controlled light-exposure microscopy reduces photobleaching and phototoxicity in fluorescence live-cell imaging. Nature Biotechnol 25:249–253
Kanbar G, Engels W, Nicholson GJ, Hertle R und Winkelmann G (2004) Tyramine functions as a toxin in honey bee larvae during Varroa-transmitted infection by Melissococcus pluton. FEMS Microbiol Lett 234:149–154
Lakowicz JR, Szmacinski H, Nowaczyk K, Berndt KW, Johnson ML (1992) Fluorescence lifetime imaging. Analytical Biochem 202: 316–330
Martin RM, Leonhardt H, Cardoso MC (2005) DNA labeling in living cells. Cytom Part A 67A:45–52
Martin RM, Tunnemann G, Leonhardt H, Cardoso MC (2007) Nucleolar marker for living cells. Histochem Cell Biol 127:243–251
Palmisano R (2004) Fluoreszenz-Resonanz-Energie-Transfer-basierter spezifischer Nachweis von mRNA in vitro und in situ. Dissertation, Universität Bielefeld. http:// bieson.ub.uni-bielefeld.de/volltexte/2004/476/pdf/ Dissertation_Palmisano.pdf
Presley JF (2005) Imaging the secretory pathway: the past and future impact of live cell optical techniques. Biochim Biophys Acta. 1744(3):259–272. Review
Rigler R, Elson E (2001) Fluorescence Correlation Spectroscopy. Springer Verlag
Romeis, B (1989) Mikroskopische Techniken. Böck P (ed), München: Urban und Schwarzenberg
Schwille P, Haupts U, Maiti S, Webb WW (1999) Molecular Dynamics in Living Cells Observed by Fluorescence Correlation Spectroscopy with One-and Two-Photon Excitation. Biophys J 77:2251–2265
Seisenberger G, Ried MU, Endress T, Buning H, Hallek M, Brauchle C (2001) Real-time single-molecule imaging of the infection pathway of an adeno-associated virus. Science 294(5548):1929–1932
Stockinger L (1964) Vitalfärbung und Vitalfluorochromierung tierischer Zellen. In: Protoplasmatologia Handbuch der Protoplasmaforschung. Alfert M, Bauer H, Harding CV. (eds), Wien: Springer Verlag.
Literatur Kapitel 2.1.4 Originalliteratur
Adams JC (1981) Heavy metal intensification of DAB-based HRP reaction product. J Histochem Cytochem 29:775
Bender KJ, Rangel J und Feldman DE (2003) Development of columnar topography in the excitatory layer 4 to layer 2/3 projection in rat barrel cortex. J Neurosci 23:8759–8770
Chen S und Aston-Jones G (1998) Axonal collateral-collateral transport of tract tracers in brain neurons: false anterograde labelling and useful tool. Neuroscience 82:1151–1163
Dugladze T, Heinemann U, Gloveli T (2001) Entorhinal cortex projection cells to the hippocampal formation in vitro. Brain Res 905:224–231
Hellmann B, Manns M und Güntürkün O (2001) Nucleus isthmi, pars semilunaris as a key component of the tectofugal visual system in pigeons. J Comp Neurol 436:153–166
Hsu SM und Raine L (1981) Protein A, avidin, and biotin in immunohistochemistry. J Histochem Cytochem 29:1349–1353
Köbbert C, Apps R, Bechmann I, Lanciego JL, Mey J, Thanos S (2000) Current concepts in neuroanatomical tracing. Prog Neurobiol 62:327–351 (http://www.unav.es/neurologia/ Lanciego/kobbert00.htm#_Toc467309232)
Kelso SR, Nelson DO, Silva NL und Boulant JA (1983) A slice chamber for intracellular and extracellular recording during continuous perfusion. Brain Res Bull 10:853–857
Kreck G, Nixdorf-Bergweiler BE (2005) Evidence for a cortical-basal ganglia projection pathway in female zebra finches. NeuroReport 16(1):21–24.
Luppi PH, Fort P und Jouvet M (1990) Iontophoretic application of unconjugated cholera toxin B subunit (CTb) combined with immunohistochemistry of neurochemical substances: a method for transmitter identification of retrogradely labeled neurons. Brain Res 534:209–224
Oztas E (2003) Neuronal tracing. Neuroanatomy 2:2–5 (http://www.neuroanatomy.org/2003/002_005.pdf)
Sakanaka M, Shibasaki T und Lederis K (1987) Improved fixation and cobalt-glucose oxidase-diaminobenzidine intensification for immunohistochemical demonstration of corticotropin-releasing factor in rat brain. J Histochem Cytochem 35:207–212
Schmued LC und Snavely LF (1993) Photoconversion and electron microscopic localization of the fluorescent axon tracer fluoro-ruby (rhodamine-dextran-amine). J Histochem Cytochem 41:777–782
Shu SY, Ju G und Fan LZ (1988) The glucose oxidase-DABnickel method in peroxidase histochemistry of the nervous system. Neurosci Lett 85:169–171
Staiger JF, Schubert D, Zuschratter W, Kotter R, Luhmann HJ und Zilles K (2002) Innervation of interneurons immunoreactive for VIP by intrinsically bursting pyramidal cells and fast-spiking interneurons in infragranular layers of juvenile rat neocortex. Eur J Neurosci 16:11–20
Veenman CL, Reiner A und Honig MG (1992) Biotinylated dextran amine as an anterograde tracer for single-and double-labeling studies. J Neurosci Methods 41:239–254
von Bohlen und Halbach O, Dermietzel R (1999). Methoden der Neurohistologie. Spektrum Akademischer Verlag GmbH, Heidelberg
Zusammenfassende Literatur und nützliche Links
Manns M (1998) Die Ontogenese visueller Lateralisation bei der Taube (Columba Livia): Entwicklung und Plastizität des Systems. Dissertation: Ruhr-Universität Bochum, Fakultät für Psychologie (http://www-brs.ub.ruhr-unibochum. de/netahtml/HSS/Diss/MannsMartina/)
von Bohlen und Halbach O und Dermietzel R (1999). Methoden der Neurohistologie. Spektrum Akademischer Verlag GmbH.
Wilhelmi E (2000). Die neuronale Verschaltung der thalamoamygdalofugalen Projektion zum cholinergen basalen Vorderhirn (Substantia innominata). Dissertation: Otto-von-Guericke-Universität Magdeburg. (http://diglib.unimagdeburg. de/Dissertationen/2000/ecwilhelmi.pdf)
Literatur Kapitel 2.2
Alexander RR and Griffiths JM (1993) Basic Biochemical Methods. Wiley-Liss, Inc, New York
Darzynkiewicz Z, Robinson JP, Crissman HA (1994) Flow Cytometry. 2nd Ed., Academic Press, San Diego
Dashek WV (2000) Methods for the Identification of isolated Plant Cell Organelles. In: Dashek WV (Ed.) Methods in Plant Electron Microscopy and Cytochemistry. Humana Press, Totowa, New Jersey
Harrison DD und Webster HL (1969) The preparation of isolated intestinal crypt cells. Exptl Cell Res 55: 257–260
Radbruch A (2000) Flow Cytometry and Cell Sorting. Springer Verlag, Heidelberg
Robinson DG and Hinz G (2001) Organelle isolation. In: Hawes C and Satiat-Jeunemaitre B (Eds) Plant Cell Biology, 2nd Edition, Oxford University Press
Romeis B (1968) Mikroskopische Technik. 16. Auflage. R Oldenbourg Verlag, München, Wien
Shapiro, HM (2003) Practical Flow Cytometry 4th Edition. Wiley-Liss, New York
Towler CM, Pugh-Humphreys GP and Porteau JW (1978) Characterization of columnar absorptive epithelial cells isolated from rat jejunum. J Cell Sci 29: 53–75
Literatur Kapitel 2.3
Chu W-S, Liang Q, Tang Y, King R, Wong K, Gong M, Wei M, Liu J, Feng S-H, Lo S-C, Andriko J-A and Orr M (2006) Ultrasound-acceralted tissue fixation/processing achieves superior morphology and macromolecule integrity with storage stability. J Histochem Cytochem 54:503–513
Giberson RT and Demaree RS Jr (Eds) (2001) Microwave techniques and protocols. Humana Press, Totowa, New Jersey
Haidenhain M (1908) Über Vanadiumhämatoxylin, Pikroblauschwarz und Kongo-Korinth. Z Wiss Mikr 25:401–410
Hauser M (1978) Demonstration of membrane-associated and oriented microfibrils in Amoeba proteus by means of a Schiff base/glutaraldehyde fixative. Cytobiologie 18:95–106
Hayat MA (1989) Principles and Techniques of Electron Microscopy. Biological Applications. Third Edition, CRC Press Inc., Boca Raton, Florida
Kardasewitsch B (1925) Eine Methode zur Beseitigung der Formalinsedimente (Paraform) aus mikroskopischen Präparaten. Z Wiss Mikr 42:322–324
Karnovsky MJ (1965) A formaldehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy. J Cell Biol 27:137a
Zakolar M and Erk I (1977) Phase-partition fixation and staining of Drosophila eggs. Stain Technology 52:89–95
Literatur Kapitel 2.4.1
Bennett H S, Wyrick A D, Lee S W, McNeil J H (1976) Science and art inpreparing tissues embedded in plastic for light microscopy, with special reference to glycerol methacrylate, glass knifes and simple stains. Stain Technol 51: 71–97
Chesterman W, Leach E H (1949) Low viscosity nitrocellulose for embedding tissues. Quart J Micr Sci 20:431–434
Dietrich A (1929) Isopropylalkohol für histologische Zwecke. Zbl allg Path pathol Anat 47: 83
Doxtader E K (1948) Isopropyl-Alcohol in the paraffin infiltration technique. Stain technol 23: 1–2.
Gerrits P O, Smid L (1983) A new, less toxic polymerization system for the embedding of soft tissues in glycolmethacrylate and subsequent preparing of serial sections. J Microscopy 132: 81–85
Lamb R A (1973) Waxes for histology. In: Histopathology, selected topics. (H C Cook, ed), p 123. Bailliére Tindall, London
Robinson H D, Fayen A W (1965) One-hour processing of tissue. Am J clin Path 43:91–92
Romeis B (1968) Mikroskopische Technik. 16. Ed. R Oldenbourg Verlag, München, Wien
Romeis B (1989) Mikroskopische Technik. 17. Ed. R Oldenbourg Verlag, München, Wien, Baltimore
von Apáthy S (1912) Neuere Beiträge zur Schneidetechnik. Z wiss Mikr: 449–515
Literatur Kapitel 2.4.2 Zusammenfassende Literatur
Bozzola JJ and Russell LD (1998) Electron Microscopy. Principles and Techniques for Biologists. Jones & Bartlett Publishing
Dashek WV (Ed) (2000) Methods in Plant Electron Microscopy and Cytochemistry. Humana Press
Glauert AM and Lewis PR (Eds) (1998) Biological Specimen Preparation for Transmission Electron Microscopy (Practical Methods in Electron Microscopy). Portland Press Ltd
Hayat MA (1989) Principles and Techniques of Electron Microscopy. Biological Applications. Third Edition, CRC Press Inc., Boca Raton, Florida
Hayat MA (2000) Principles and Techniques of Electron Microscopy. Biological Applications. Fourth Edition, Cambridge University Press
Einzelpublikationen
Brilakis HS, Hann CR and Johnson DH (2001) A comparison of different embedding media on the ultrastructure of the trabecular meshwork. Curr Eye Res 22(3):235–244
Hillmer S, Joachim S and Robinson DG (1991) Rapid polymerization of LR-white for immunocytochemistry. Histochemistry 95:315–318
Richardson KC, Jarrett L and Finke EH (1960) Embedding in epoxy resins for ultrathin sectioning in electron microscopy. Stain Technology 35:313
Steiner M, Schöfer C and Mosgoeller W (1994) In situ flat embedding of monolayers and cell relocation in the acrylic resin LR White for comparative light and electron microscopy. Histochem J 26:934–938
Tolivia J, Navarro A and Tolivia D (1994) Polychromatic staining of epoxy sections: a new and simple method. Histochemistry 101:51–55
Literatur Kapitel 2.5
Barlow, D.I. and Sleigh, M.A. (1979) Freeze substitution for preservation of ciliated surfaces for scanning electron microscopy. J. Microscopy 115: 81–95.
Goldstein J, Newbury DE, Joy DC, Lyman CE, Echlin P, Lifshin E, Sawyer LC and Michael JR (2003) Scanning Electron Microscopy and X-ray Microanalysis. 3. Ed Springer
Eisenbeis, G. und Wichard, W. (1985) Atlas zur Biologie der Bodenarthropoden, Gustav Fischer Verlag.
Ensikat, H.J. and Barthlott, W. (1993) Liquid substitution: a versatile procedure für SEM specimen preparation of biological materials without drying or coating. J. Microscopy 172: 195–203.
Flegler SL, Heckman JW, Klomparens KL (1995) Scanning and Transmission Electron Microscopy: An Introduction Oxford University Press, USA
Hayes, T.L. (1973) Scanning Electron microscope Techniques in Biology. In: Koehler, J.K (Ed) Advanced Techniques in Biological Electron Microscopy. Springer-Verlag, Berlin, Heidelberg, New York.
Ohnesorge, J. und Holm, R. (1978) Rasterelektronenmikroskopie — Eine Einführung für Mediziner und Biologen. Scanning Electron Microscopy — An Introduction for Physicians and Biologists. 2nd Ed. Georg Thieme Publishers Stuttgart
Reimer, L. und Pfefferkorn, G. (1973) Raster-Elektronenmikroskopie. Springer-Verlag, Berlin, Heidelberg, New York.
Rosenbauer, K.A. und Kegel, B.H. Rasterelektronenmikroskopische Technik, Päparationsverfahren in Medizin und Biologie, (1978), Thieme Verlag.
Wallace, I.P. and Fischman, D.A. (1979) High resulotion scanning electron microscopy of isolated and in situ cytoskeletal elements. J. Cell Biol. 83: 249–254.
Wichard, W., Arens, W. und Eisenbeis, G. (1995) Atlas zur Biologie der Wasserinsekten, Gustav Fischer Verlag.
Literatur Kapitel 2.6 Zusammenfassende Literatur
Brooks SA, Leathem AJC and Schumacher U (1997) Lectin Histochemistry. A concise practical handbook. BIOS Scientific Publishers Limited, Oxford
Hayat MA (1989) Principles and Techniques of Electron Microscopy. Biological Applications. Third Edition, CRC Press Inc., Boca Raton, Florida
Hayat MA and Miller SE (1990) Negative Staining. McGraw-Hill Inc., US
Lewis PR (1977) Staining Methods for Sectioned Material. Practical Methods in Electron Microscopy, vol 5, Elsevier Science Ltd.
Neiss WF (1986) Ultracytochemistry of intracellular membrane glycoconjugates. Advances in Anatomy, Embryology and Cell Biology vol. 99, Springer-Verlag, Berlin, Heidelberg, New York
Scott JE (1985) Proteoglycan Histochemistry — a Valuable Tool for Biochemists. Coll Rel Res 5, 541–598
Wohlrab F und Gossrau R (1992) Katalytische Enzymhistochemie. Grundlagen und Methoden für die Elektronenmikroskopie. Gustav Fischer Verlag, Jena
Originalartikel
Bestwick CS, Brown IR, Bennet MHR and Mansfield JW (1997) Localization of hydrogen peroxide accumulation during the hypersensitive reaction of lettuce cells to Pseudomonas syringae pv phaseolicola. The Plant Cell 9:209–221
Hirabayashi Y, Sakagami T and Yamada K (1990) Electron microscopic visualization of acidic glycoconjugates by means of postembedding procedures using ruthenium red and tungstate. Acta Histochem Cytochem 23:165–173
Mentré P and Halpern S (1988a) Localization of cations by pyroantimonate. I. Influence of fixation on distribution of calcium and sodium. An approach by analytical ion microscopy. J Histochem Cytochem 36:49–54
Mentré P and Halpern S (1988b) Localization of cations by pyroantimonate. II. Electron probe microanalysis of calcium and sodium in skeletal muscle of mouse. J Histochem Cytochem 36:55–64
Reynolds ES (1963) The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J Cell Biol 17:208
Spicer SS and Schulte BA (1992) Diversity of cell glycoconjugates shown histochemically: a perspective. J Histochem Cytochem 40:1–38
Literatur Kapitel 2.7
Carlemalm E, Garavito RM and Villinger W (1982) Resin development for electron microscopy and an analysis of embedding at low temperature. J Microsc 126:123–143
Costello MJ, Fetter R and Höchli M (1981) Simple procedures for evaluating the cryofixation of biological samples. J Microsc 125:125–136
Fernandez-Moran H (1952) Application of the ultrathin freezing-sectioning technique to the study of cell structures with the electron microscope. Ark Fys 4:471–491
Hohenberg H, Mannweiler K and Müller M (1994) High-pressure freezing of cell suspensions in cellulose capillary tubes. J Microsc. 175:34–43
McIntyre JA, Gilula NB and Karnovsky MJ (1974) Cryoprotectant-induced redistribution of intramembranous particles in mouse lymphocytes. J Cell Biol 60:192–203
Moor H, Bellin G, Sandri C and Akert K (1980) The influence of high pressure freezing on mammalian nerve tissue. Cell Tissue Res 209:201–216
Moor H (1987) Theory and practise of high pressure freezing. In: Steinbrecht RA Zierold K (Eds) Cryotechniques in biological electron microscopy. Springer, Berlin Heidelberg New York pp 175–191
Müller M, Meister N and Moor H (1980) Freezing in a propane jet and its application in freeze-fracturing. Mikroskopie (Wien) 36: 129–140
Müller M and Moor H (1984) Cryofixation of thick specimens by high pressure freezing. Scanning Electron Microsc 1984:131–138
Müller-Reichert T, Hohenberg H, O’Toole ET and McDonald K (2003) Cryoimmobilization and three dimensional visualization of C. elegans ultrastructure. J Microsc 212:71–80
Pfeiffer S, Vielhaber G, Vietzke JP, Wittern KP, Hintze U, and Wepf R (2000) High-pressure freezing provides new information on human epidermis: Simultaneous protein antigen and lamellar lipid structure preservation. Study on human epidermis by cryoimmobilization. J Invest Dermatol 114:1030–1038
Pfeiffer S and Krupinska K (2005) Chloroplast Ultrastructure in Leaves of Urtica dioica L. Analyzed after Highpressure freezing and freeze-substitution and compared with conventional fixation followed by room temperature dehydration. Microscopy Research and Technique 68:368–379
Plattner H and Bachmann L (1982) Cryofixation of biological materials for electron microscopy by the methods of spray, sandwich-, cryogen-jet-and sandwich-cryogen-jet-freezing: a comparison of techniques. In: Revel JP, Barnard T Haggis GH (Eds) Science of biological specimen preparation, Vol 2 Chicago, SEM Inc, AMF O’Hare, Chicago. 139–147
Riehle U and Höchli M (1973) The theory and technique of high pressure freezing. In: Benedetti EL and Favard P (Eds) Freeze etching technique and application. Société Francaise de Microscopie Electronique, Paris. 31–60
Studer D, Graber W, AlAmoudi A and Eggli P (2001) A new approach for cryofixation by high-pressure freezing machine. J Microsc 203:285–294
Tokuyasu KT (1973) A technique for ultracrytomy of cell suspensions and tissues. J Cell Biol 57: 551–565
Welter K, Müller M and Mendgen K (1988) The hyphae of Uromyces appendiculatus within the leaf tissue after high pressure freezing and freeze substitution. Protoplasma 147:91–99
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Aescht, E. et al. (2010). Präparationsmethoden. In: Mulisch, M., Welsch, U. (eds) Romeis Mikroskopische Technik. Spektrum Akademischer Verlag. https://doi.org/10.1007/978-3-8274-2254-5_2
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