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Deposition of Scar Tissue in the Central Nervous System

  • Conference paper
Trauma and Regeneration

Part of the book series: Acta Neurochirurgica Supplementum ((NEUROCHIRURGICA,volume 32))

Summary

Standard parasagittal lesions were placed stercotactically in the cerebral hemispheres of neonatal and adult rats in order to compare scarring in the immature and mature animal. Lesions were examined by light and electron-microscopy and immunofluorescence to study the astrocyte reaction, collagen deposition, and the formation of the basement memebrane of the glia limitans.

Normal mature scarring characterized by the deposition of collagen, astrocyte end-feet alignment over a glia limitans, and the permanent presence of mesodermal cells (fibroblasts and macrophages) in the core of the lesion, does not occur in wounds before 8–10 days post-partum (dpp). Instead there is no deposition of collagen, and only a transitory astrocyte response occurs with the formation of an interrupted glia limitans. These latter features disappear with time so that the wound is ultimately obliterated by the growth of axons and dendrites through the lesion. Mature scarring is attained over 8–12 dpp when increasing amounts of collagen are deposited and a continuous permanent glia limitans is formed.

The acquisition of the mature response to injury from 8–12 dpp may be correlated with the presence of increasing titres of a fibroblast growth factor (FGF), derived from autolytic digestion of injured brain tissue. We have investigated FGF activity using a 3 T 3 fibroblast tissue culture assay to detect mitogenic activity in brain extracts from rats lesioned at different ages and from leukodystrophic mice which have no myelin.

Our results show that high titres of FGF are present in the developing brain long before myelination commences, and that normal levels of FGF are found in the brains of leukodystrophic mice which have no myelin. Scarring in brain lesions in these mutants is quite normal.

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Authors and Affiliations

  1. Anatomy Department, Guy’s Hospital Medical School, London, SE1 9RT, UK

    Prof. Dr. M. Berry & Dr. A. Mathewson

  2. Department of Anatomy, St. George’s Hospital Medical School, London, SW 17ORE, UK

    Dr. W. L. Maxwell & Doreen E. Ashhurst

  3. Department of Human Anatomy, University of Oxford, South Parks Road, Oxford, OX1 3QX, UK

    Dr. P. Mcconnell

  4. Department of Anatomy, University of Birmingham, Birmingham, B15 2TJ, UK

    Dr. A. Logan & Dr. G. H. Thomas

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  1. University Department of Neuropathology, Institute of Neurological Sciences, Glasgow, Scotland, UK

    J. Hume Adams M.B., Ch.B., Ph.D., F.R.C.Path., F.R.C.P. (Professor of Neuropathology, Secretary-General, International Society of Neuropathology) (Professor of Neuropathology, Secretary-General, International Society of Neuropathology)

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© 1983 Springer-Verlag Wien

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Berry, M. et al. (1983). Deposition of Scar Tissue in the Central Nervous System. In: Adams, J.H. (eds) Trauma and Regeneration. Acta Neurochirurgica Supplementum, vol 32. Springer, Vienna. https://doi.org/10.1007/978-3-7091-4147-2_3

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  • DOI: https://doi.org/10.1007/978-3-7091-4147-2_3

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-211-81775-9

  • Online ISBN: 978-3-7091-4147-2

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