Abstract
Background: Although there has been much work on it for years, cerebral vasospasm as a complication of subarachnoid bleeding is still an important cause of mortality and morbidity. The presented study was designed to examine the effects of heme oxygenase inhibitor, Zn (II) protoporphyrin IX, on vasospasm process.
Method: In this study 20 male New Zealand White rabbits weighing 2,500 to 3,000 g were randomly assigned to four groups. Animals in group 1 were controls. In group 2, animals were SAH induced only and no treatment given. SAH induced animals in group 3 received intracisternal Zn (II) protoporphyrin IX 0.5 mg/kg in DMSO solution, at 0 and 24 h of SAH induction. In group 4, animals received only intracisternal DMSO at 0 and 24 h after SAH induction. All animals were sacrificed via exsanguination at 72 h after induction of SAH. Brains and brainstems with overlying basilar arteries were removed and stored in fixative at +4°C overnight. Basilar arteries were sectioned from four seperate zones, and four sections were obtained from each rabbit. Basilar artery luminal section areas and vessel wall thicknesses were measured by using SPOT for Windows version 4.1. Statistical comparisons were performed using Mann-Whitney and ANOVA tests.
Findings: Basilar arterial wall thicknesses in group 3 were smaller than that of group 2 (p < 0.05). Luminal section areas in group 3 were significantly greater than luminal section areas of group 2 (p < 0.05).
Conclusion: ZnPP has a potentially beneficial effect on cerebral vasospasm after subarachnoid bleeding.
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References
Bilginer B, Onal B, Yigitkanli K, Soylemezoglu F, Bavbek M, Ziyal IM, et al. Treatment of cerebral vasospasm with cilostazol in subarachnoid haemorrhage model. Acta Neurochirurgica Suppl. 2008;104:291–6.
Clark JF, Reilly M, Sharp FR. Oxidation of bilirubin produces compounds that cause prolonged vasospasm of rat cerebral vessels: a contributor to subarachnoid hemorrhage-induced vasospasm. J Cereb Blood Flow Metab. 2002;22:472–8.
Clark JF, Sharp FR. Bilirubin oxidation products (BOXes) and their role in cerebral vasospasm after subarachnoid hemorrhage. J Cereb Blood Flow Metab. 2006;26:1223–33.
Furchgott RF, Vanhoutte PM. Endothelium-derived relaxing and contracting factors. FASEB J. 1989;3:2007–18.
Gong Y, Tian H, Xi G, Keep RF, Hoff JT, Hua Y. Systemic zinc protoporphyrin administration reduces intracerebral hemorrhage-induced brain injury. Acta Neurochir Suppl. 2006;96:232–6.
Grasso G. An overview of new pharmacological treatments for cerebrovascular dysfunction after experimental subarachnoid hemorrhage. Brain Res Brain Res Rev. 2004;44:49–63.
Kadoya C, Domino EF, Yang GY, Stern JD, Betz AL. Preischemic but not postischemic zinc protoporphyrin treatment reduces infarct size and edema accumulation after temporary focal cerebral ischemia in rats. Stroke 1995;26:1035–8.
Kassell NF, Torner JC, Haley EC Jr, Jane JA, Adams HP, Kongable GL. The International Cooperative Study on the Timing of Aneurysm Surgery. Part 1: overall management results. J Neurosurg. 1990;73:18–36.
Kranc KR, Pyne GJ, Tao L, Claridge TD, Harris DA, Cadoux-Hudson TA, et al. Oxidative degradation of bilirubin produces vasoactive compounds. Eur J Biochem. 2000;267:7094–101.
Mayberg MR. Cerebral vasospasm. Neurosurg Clin N Am. 1998;9:615–27.
Pluta RM. Delayed cerebral vasospasm and nitric oxide: review, new hypothesis, and proposed treatment. Pharmacol Ther. 2005;105:23–56.
Pyne-Geithman GJ, Morgan CJ, Wagner K, Dulaney EM, Carrozzella J, Kanter DS, et al. Bilirubin production and oxidation in CSF of patients with cerebral vasospasm after subarachnoid hemorrhage. J Cereb Blood Flow Metab. 2005;25:1070–7.
Sahoo SK, Sawa T, Fang J, Tanaka S, Miyamoto Y, Akaike T, et al. Pegylated zinc protoporphyrin: a water-soluble heme oxygenase inhibitor with tumor-targeting capacity. Bioconjug Chem. 2002;13:1031–8.
Zhao YJ, Yang GY, Domino EF. Zinc protoporphyrin, zinc ion, and protoporphyrin reduce focal cerebral ischemia. Stroke 1996;27:2299–303.
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Isikay, I., Bilginer, B., Narin, F., Soylemezoglu, F., Akalan, N. (2011). The Effect of Intracisternal Zn (II) Protoporphyrin IX on Vasospasm Process in the Experimental Subarachnoid Hemorrhage Model. In: Feng, H., Mao, Y., Zhang, J.H. (eds) Early Brain Injury or Cerebral Vasospasm. Acta Neurochirurgica Supplements, vol 110/2. Springer, Vienna. https://doi.org/10.1007/978-3-7091-0356-2_7
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DOI: https://doi.org/10.1007/978-3-7091-0356-2_7
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