Abstract
Background: Osteopontin (OPN), a pleiotropic extracellular matrix glycoprotein, has been reported to have neuroprotective effects against early brain injury after subarachnoid hemorrhage (SAH). The aim of this study is to examine if osteopontin prevents cerebral vasospasm after SAH in rats.
Method: The endovascular perforation model of SAH was produced, and 62 rats were randomly assigned to sham + vehicle, SAH + vehicle, and SAH+ r-OPN (0.1 μg) groups. Cerebral vasospasm was evaluated by India ink angiography at 24 and 72 h after SAH, as well as neurobehavioral tests.
Findings: Significant vasospasm and neurological impairments occurred over the observed period after SAH. r-OPN significantly prevented vasospasm in the left middle cerebral artery at 24 h and improved neurological impairments at 48 h after SAH. In other time points studied, r-OPN had a tendency toward improving both vasospasm and neurological scores, but the difference was not significant.
Conclusions: This study shows that r-OPN has anti-vasospastic effects against cerebral vasospasm after SAH.
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References
Xie Y, Sakatsume M, Nishi S, Narita I, Arakawa M, Gejyo F. Expression, roles, receptors, and regulation of osteopontin in the kidney. Kidney Int. 2001;60:1645–1657.
Mazzali M, Kipari T, Ophascharoensuk V, Wesson JA, Johnson R, Hughes J. Osteopontin. A molecule for all seasons. Q J Med. 2002;95:3–13.
Suzuki H, Ayer R, Sugawara T, Chen W, Sozen T, Hasegawa Y, et al. Protective effects of recombinant osteopontin on early brain injury after subarachnoid hemorrhage in rats. Crit Care Med. 2010;38:612–618.
Sugawara T, Ayer R, Jadhav V, Zhang JH. A new grading system evaluating bleeding scale in filament perforation subarachnoid hemorrhage rat model. J Neurosci Meth. 2008;167:327–334.
Parra A, McGirt MJ, Sheng H, Laskowitz DT, Pearlstein RD, Warner DS. Mouse model of subarachnoid hemorrhage associated cerebral vasospasm: methodological analysis. Neurol Res. 2002;24:510–516.
Zheng J-S, Zhan R-Y, Zheng S-S, Zhou Y-Q, Tong Y, Wan S. Inhibition of NADPH oxidase attenuates vasospasm after experimental subarachnoid hemorrhage in rats. Stroke 2005;36:1059–1064.
Arafat HA, Katakam AK, Chipitsyna G, Gong Q, Vancha AR, Gabbeta J, et al. Osteopontin protects the islets and beta-cells from interleukin-1 beta-mediated cytotoxicity through negative feedback regulation of nitric oxide. Endocrinology 2007;148:575–584.
Guo H, Wai PY, Mi Z, Gao C, Zhang J, Kuo PC. Osteopontin mediates Stat1 degradation to inhibit iNOS transcription in a cecal ligation and puncture model of sepsis. Surgery 2008;144:182–188.
Pannu R, Singh I. Pharmacological strategies for the regulation of inducible nitric oxide synthase. Neurodegenerative versus neuroprotective mechanisms. Neurochem Int. 2006;49:170–182.
Xie Z, Singh M, Siwik DA, Joyner WL, Singh K. Osteopontin inhibits interleukin-1beta-stimulated increases in matrix metalloproteinase activity in adult rat cardiac fibroblasts: role of protein kinase C-zeta. J Biol Chem. 2003;278:48546–48552.
Acknowledgments
This study was partially supported by grants (NS053407) from the National Institutes of Health to J.H.Z.
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Suzuki, H., Hasegawa, Y., Kanamaru, K., Zhang, J.H. (2011). Effect of Recombinant Osteopontin on Cerebral Vasospasm After Subarachnoid Hemorrhage in Rats. In: Feng, H., Mao, Y., Zhang, J.H. (eds) Early Brain Injury or Cerebral Vasospasm. Acta Neurochirurgica Supplements, vol 110/2. Springer, Vienna. https://doi.org/10.1007/978-3-7091-0356-2_6
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DOI: https://doi.org/10.1007/978-3-7091-0356-2_6
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