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Effect of a Free Radical Scavenger, Edaravone, on Free Radical Reactions: Related Signal Transduction and Cerebral Vasospasm in the Rabbit Subarachnoid Hemorrhage Model

  • Conference paper
Early Brain Injury or Cerebral Vasospasm

Part of the book series: Acta Neurochirurgica Supplements ((NEUROCHIRURGICA,volume 110/2))

Abstract

Objective: It is hypothesized that free radical reactions evoked by oxyhemoglobin (oxyHb) cause cerebral vasospasm after aneurysmal subarachnoid hemorrhage (SAH), even though the detailed mechanisms have not yet been fully established. The aims of this study were thus to investigate, through the use of the double-hemorrhage rabbit model, the possibility that free radical reactions play a role in cerebral vasospasm and to delineate the mechanism of signal transduction that causes cerebral vasospasm.

Methods: In the SAH group, SAH was simulated using the double-hemorrhage rabbit model. In the treatment group, edaravone (0.6 mg/kg), a potent free radical scavenger, was injected into the central ear vein twice a day. Four days after SAH, the basilar artery was excised. The degree of cerebral vasospasm was evaluated by measuring the diameter of each basilar artery, and the expression of Rho-kinase in the vascular wall was examined by western blotting.

Results: The diameter of the basilar artery in the edaravone-treated group was 0.64 ± 0.06 mm, which was statistically significantly larger than that in the nontreated SAH group (0.50 ± 0.03 mm; p < 0.01). The expression of Rho-kinase in the edaravone-treated group was statistically significantly reduced in comparison to that of the nontreated SAH group.

Conclusion: Results from this study have indicated for the first time that free radical reactions mediated by oxyHb may play an important role in the pathogenesis of cerebral vasospasm through the expression of Rho-kinase.

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Authors and Affiliations

  1. Department of Neurosurgery, Hirosaki University School of Medicine, Hirosaki, Japan

    Akira Munakata, Hiroki Ohkuma & Norihito Shimamura

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  1. Akira Munakata
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  2. Hiroki Ohkuma
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  3. Norihito Shimamura
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Correspondence to Norihito Shimamura .

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Editors and Affiliations

  1. Department of Neurosurgery, Southwest Hospital, Third Military Medical University, Gaotanyan 30, Chongqing, 400038, China

    Hua Feng

  2. Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China

    Ying Mao

  3. Departments of Physiology and Neurosurgery, Loma Linda University School of Medicine, Risley Hall, Room 223, 92354, Loma Linda, California, USA

    John H. Zhang

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Munakata, A., Ohkuma, H., Shimamura, N. (2011). Effect of a Free Radical Scavenger, Edaravone, on Free Radical Reactions: Related Signal Transduction and Cerebral Vasospasm in the Rabbit Subarachnoid Hemorrhage Model. In: Feng, H., Mao, Y., Zhang, J.H. (eds) Early Brain Injury or Cerebral Vasospasm. Acta Neurochirurgica Supplements, vol 110/2. Springer, Vienna. https://doi.org/10.1007/978-3-7091-0356-2_4

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  • DOI: https://doi.org/10.1007/978-3-7091-0356-2_4

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