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Changes of Blood–Brain Barrier Permeability Following Intracerebral Hemorrhage and the Therapeutic Effect of Minocycline in Rats

  • Conference paper
Early Brain Injury or Cerebral Vasospasm

Part of the book series: Acta Neurochirurgica Supplements ((NEUROCHIRURGICA,volume 110/2))

Abstract

Objective: To investigate the changes of blood–brain barrier (BBB) permeability and expressions of VEGF, NGF and HPS70 in brain at different time points following intracerebral hemorrhage (ICH) in rats, and observe therapeutic effect of minocycline (MC).

Methods: Rat ICH model was induced with Type IV collagenase. Early MC treatment was administrated via intraperitoneal injection. BBB permeability was evaluated by Evans blue (EB) amount exuded out of cerebral vessels. VEGF, NGF, and HPS70 expressions were determined with immunohistochemical staining.

Results: EB exudation amount in MC treatment group was less than the ICH group (P < 0.05). The former showed a transient EB exudation peak only 1 h after modeling and then gradually decreased, while the latter showed two EB exudation peaks 1 and 4 days after modeling, respectively. The number of VEGF-positive cells in MC treatment group was less than the ICH group (P < 0.05), whereas the number of NGF- and HSP70-positive cells were more than the ICH group (P < 0.05). All three were mainly expressed in neurons and gitter cells, but there were only few expressions in the control group.

Conclusion: After ICH, the BBB permeability was destroyed, with neuron function affected. In the early stage, VEGF increased BBB permeability, while NGF and HSP70 showed protective effects on nerve cells. Early intraperitoneal injection with MC could reduce the damage of BBB and increase the protective effect on nerve cells, the mechanism of which may be achieved by reducing VEGF expression and enhancing NGF and HSP70 expressions.

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Abbreviations

BBB:

Blood–brain barrier

ICH:

Intracerebral hemorrhage

IP:

Ischemic penumbra

MC:

Minocycline

VEGF:

Vascular endothelial cell growth factor

VEC:

Vascular endothelial cell

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Authors and Affiliations

  1. Department of Neurosurgery, Second Affiliated Hospital of Xi’an Jiaotong University, 157 Xiwu Road, Xi’an, 710004, P.R, China

    Wei Shi, Zizhang Wang, Jingnan Pu, Ruizhi Wang, Zhengyu Guo, Chongxiao Liu, Jianjun Sun, Ligui Gao & Ren Zhou

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  1. Wei Shi
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  2. Zizhang Wang
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  3. Jingnan Pu
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  4. Ruizhi Wang
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  5. Zhengyu Guo
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  6. Chongxiao Liu
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  7. Jianjun Sun
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  8. Ligui Gao
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  9. Ren Zhou
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Correspondence to Wei Shi .

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Editors and Affiliations

  1. Department of Neurosurgery, Southwest Hospital, Third Military Medical University, Gaotanyan 30, Chongqing, 400038, China

    Hua Feng

  2. Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China

    Ying Mao

  3. Departments of Physiology and Neurosurgery, Loma Linda University School of Medicine, Risley Hall, Room 223, 92354, Loma Linda, California, USA

    John H. Zhang

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Shi, W. et al. (2011). Changes of Blood–Brain Barrier Permeability Following Intracerebral Hemorrhage and the Therapeutic Effect of Minocycline in Rats. In: Feng, H., Mao, Y., Zhang, J.H. (eds) Early Brain Injury or Cerebral Vasospasm. Acta Neurochirurgica Supplements, vol 110/2. Springer, Vienna. https://doi.org/10.1007/978-3-7091-0356-2_12

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  • DOI: https://doi.org/10.1007/978-3-7091-0356-2_12

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-7091-0355-5

  • Online ISBN: 978-3-7091-0356-2

  • eBook Packages: MedicineMedicine (R0)

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