Abstract
Glycan microarray allows for direct measurement and characterization of the carbohydrate–protein interactions and only a very small amount of sugar is required. In addition, it may not only effectively mimic the presentation of glycans on the cell membrane but also yields high affinity and specificity to exhibit the multivalent interaction with receptor. With these synthetic carbohydrates in hand, the structure of the synthetic product was verified by 1D and 2D NMR analysis. Besides, we also used the RM2 antibody for binding analysis on a glycan array to confirm our synthetic structure. Furthermore, we use carbohydrate microarray to further characterize the binding specificities of these RM2 and its truncated analogs with monoclonal antibody RM2.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Saito S et al (2005) RM2 antigen (beta 1,4-GalNAc-disialyl-Lc(4)) as a new marker for prostate cancer. Int J Cancer 115(1):105–113
Ito A et al (2001) Monoclonal antibody (5F3) defining renal cell carcinoma-associated antigen disialosyl globopentaosylceramide (V(3)NeuAcIV(6)NeuAcGb5), and distribution pattern of the antigen in tumor and normal tissues. Glycoconj J 18(6):475–485
Fukushi Y et al (1986) A novel disialoganglioside (iv3neuaciii6neuaclc4) of human adenocarcinoma and the monoclonal-antibody (fh9) defining this disialosyl structure. Biochemistry 25(10):2859–2866
Ito A et al (2001) Binding specificity of siglec7 to disialogangliosides of renal cell carcinoma: possible role of disialogangliosides in tumor progression (vol 498, pg 116, 2001). FEBS Lett 504(1–2):81–86
Chuang HY et al (2013) Synthesis and vaccine evaluation of the tumor-associated carbohydrate antigen RM2 from prostate cancer. J Am Chem Soc 135(30):11140–11150
Liang PH, Wang SK, Wong CH (2007) Quantitative analysis of carbohydrate-protein interactions using glycan microarrays: determination of surface and solution dissociation constants. J Am Chem Soc 129(36):11177–11184
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
Copyright information
© 2015 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Chuang, HY. (2015). RM2 Antigen: Structural Characterization and Determination of K D,Surf for Multivalent Carbohydrate–Protein Interaction. In: Synthesis and Vaccine Evaluation of the Tumor Associated Carbohydrate Antigen RM2 from Prostate Cancer. Springer Theses. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-46848-7_2
Download citation
DOI: https://doi.org/10.1007/978-3-662-46848-7_2
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-662-46847-0
Online ISBN: 978-3-662-46848-7
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)