Abstract
Regeneration of fertile plants from protoplasts of sunflower (Helianthus annuus L.) has met with considerable difficulties in spite of numerous efforts over several years. The isolation of protoplasts and their culture up to the callus stage does not appear to present major difficulties (Bohorova et al. 1986; Lenée and Chupeau 1986; Moyne et al. 1988; Chanabé et al. 1989; Guilley and Hahne 1989; Schmitz and Schnabl 1989; Dupuis et al. 1990). Most currently used donor materials, including hypocotyl, cotyledons, petioles, leaves, and suspension cultures, allow the isolation of protoplasts at comparatively high yields, and media have been developed for the regeneration of callus with good plating efficiencies from all of these types.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Barth S, Voeste D, Schnabl H (1993) Somatic hybrids of sunflower (Helianthus annuus L.) identified at the callus stage by isoenzyme analysis. Bot Acta 106: 100–102
Binding H, Nehls R, Kock R, Finger J, Mordhorst G (1981) Comparative study on protoplast regeneration in herbaceous species of the Dicotyledoneae class. Z Pflanzenphysiol 101: 119–130
Bohorova NE, Cocking EC, Power JB (1986) Isolation, culture and callus regeneration of proto-plasts of wild and cultivated Helianthus species. Plant Cell Rep 5: 256–258
Burrus M, Chanabé C, Alibert G, Bidney D (1991) Regeneration of fertile plants from protoplasts of sunflower (Helianthus annuus L.). Plant Cell Rep 10: 161–166
Carter JF (1978) Sunflower science and technology. Am Soc Agron, Crop Sci Soc Am, Soil Sci Soc Am Madison
Chanabé C, Burrus M, Alibert G (1989) Factors affecting the improvement of colony formation from sunflower protoplasts. Plant Sci 64: 125–132
Chanabé C, Burrus M, Bidney D, Alibert G (1991) Studies on plant regeneration from protoplasts in the genus Helianthus. Plant Cell Rep 9: 635–638
Dupuis JM, Péan M, Chagvardieff P (1990) Plant donor tissue and isolation procedure effect on early formation of embryoids from protoplasts of Helianthus annuus L. Plant Cell Tissue Organ Cult 22: 183–189
Fischer C, Hahne G (1992) Structural analysis of colonies derived from sunflower (Helianthus annuus L.) protoplasts cultured in liquid and semisolid media. Protoplasma 169: 130–138
Fischer C, Klethi P, Hahne G (1992) Protoplasts from cotyledon and hypocotyl of sunflower (Helianthus annuus L.): shoot regeneration and seed production. Plant Cell Rep 11: 632–636
Fischer C, Laparra H, Hahne G (1993) Towards somatic hybrids of sunflower. Biotechnol Biotechnol Equip 7: 129–131
Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151–158
Gavin AL, Conger BV, Trigano RN (1989) Sexual transmission of somatic embryogenesis in Dactylis glomerata. Plant Breed 103: 251–254
Georgieva-Todorova J (1976) Interspecific relationships within the genus Helianthus. Bulg Acad Sci, Sofia
Guilley E, Hahne G (1989) Callus formation from isolated sunflower (Helianthus annuus) mesophyll protoplasts. Plant Cell Rep 8: 226–229
Kahl G, Kaemmer D, Weising K, Kost S, Weigand F, Saxena MC (1994) The potential of gene technology and genome analysis for cool season food legume crops: theory and practice. Euphytica 73: 177–189
Kirches E, Frey N, Schnabl H (1991) Transient gene expression in sunflower mesophyll protoplasts. Bot Acta 104: 212–216
Krasnyanski S, Menczel L (1993) Somatic embryogenesis and plant regeneration from hypocotyl protoplasts of sunflower (Helianthus annuus L.). Plant Cell Rep 12: 260–263
Krasnyanski S, Menczel L (1995) Production of fertile somatic hybrid plants of sunflower and Helianthus giganteus L. by protoplasts fusion. Plant Cell Rep 14: 232–235
Krasnyanski S, Polgar Z, Németh G, Menczel L (1992) Plant regeneration from callus and protoplast cultures of Helianthus giganteus L. Plant Cell Rep 11: 7–10
Laparra H, Burrus M, Hunold R, Damm B, Bravo-Angel AM, Bronner R, Hahne G (1995) Expression of foreign genes in sunflower (Helianthus annuus L.) — evaluation of three transfer methods. Euphytica 85: 63–74
Lenée P, Chupeau Y (1986) Isolation and culture of sunflower protoplasts (Helianthus annuus L.): factors influencing the viability of cell colonies derived from protoplasts. Plant Sci 43: 69–75
Moyne AL, Thor V, Pelissier B, Bergounioux C, Freyssinet G, Gadal P (1988) Callus and embryoid formation from protoplasts of Helianthus annuus. Plant Cell Rep 7: 437–440
Moyne AL, Tagu D, Thor V, Bergounioux C, Freyssinet G, Gadal P (1989) Transformed calli obtained by direct gene transfer into sunflower protoplasts. Plant Cell Rep 8: 97–100
Murashige T, Skoog F (1962) A revised medium for growth and rapid bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497
Polgar Z, Krasnyanski S (1992) Plant regeneration from cell suspension and mesophyll protoplasts of Helianthus maximiliani ( Schrad. ). Plant Sci 87: 191–197
Ray JM, Bingham ET (1989) Breeding alfalfa for regeneration from tissue culture. Crop Sci 29: 1545–1548
Schenk RU, Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot 50: 199–204
Schilling EE, Heiser CB (1981) Intrageneric classification of Helianthus Compositae. Taxon 30: 393–401
Schmitz P, Schnabl H (1989) Regeneration and evacuolation of protoplasts from mesophyll, hypocotyl, and petioles from Helianthus annuus L. J Plant Physiol 135: 223–227
Sundberg E, Glimelius K (1986) A method for production of interspecific hybrids within Brassicaceae via somatic hybridization using resynthesis of Brassica napus as a model. Plant Sci 43: 155–162
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1996 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Fischer, C., Laparra, H., Charrière, F., Jung, JL., Hahne, G. (1996). Regeneration of Plants from Protoplasts of Helianthus annuus L. (Sunflower). In: Bajaj, Y.P.S. (eds) Plant Protoplasts and Genetic Engineering VII. Biotechnology in Agriculture and Forestry, vol 38. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-09368-9_5
Download citation
DOI: https://doi.org/10.1007/978-3-662-09368-9_5
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-08240-5
Online ISBN: 978-3-662-09368-9
eBook Packages: Springer Book Archive