Abstract
This project focused on the characterization, establishment and chemoenzymatic application of the FAD-dependent halogenase Bmp5 from Pseudoalteromonas luteoviolacea. Subsequent to subcloning to enable heterologous expression in E. coli the enzyme expression, catalytic activity, substrate scope as well as Bmp5’s ability towards immobilization and suitable cofactor regeneration systems was examined to gain an overview of the brominase’s area of application.
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© 2017 Springer Fachmedien Wiesbaden GmbH
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Minges, H. (2017). Establishment of a marine brominase. In: Engineering of Halogenases towards Synthetic Applications. BestMasters. Springer Spektrum, Wiesbaden. https://doi.org/10.1007/978-3-658-18410-0_7
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DOI: https://doi.org/10.1007/978-3-658-18410-0_7
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Publisher Name: Springer Spektrum, Wiesbaden
Print ISBN: 978-3-658-18409-4
Online ISBN: 978-3-658-18410-0
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