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Polymerase Chain Reaction Diagnosis of Human Parvovirus B19

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Diagnosis of Human Viruses by Polymerase Chain Reaction Technology

Part of the book series: Frontiers of Virology ((FRVIROLOGY,volume 1))

Summary

Polymerase chain reaction (PCR) is being widely applied in virological laboratories, although it is not yet a routine technique. It has been used for the detection of parvovirus B19 DNA in serum from acute and chronic cases of infection and also for the detection of B19 DNA in blood products. Although for the diagnosis of acute infections PCR is of limited value to laboratories that already have available specific anti-B19 IgM, IgG and DNA hybridization tests, it can be established by any other laboratory that does not have access to the necessary reagents for these tests. Moreover, chronic B19 infections and their treatment can be monitored by PCR. As in most other areas of molecular biology, PCR has considerable potential as a research method for studying parvoviruses.

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Authors and Affiliations

  1. PHLS Virus Reference Laboratory, 61 Colindale Avenue, London, NW9 5HT, UK

    Jonathan P. Clewley

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  1. Jonathan P. Clewley
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Editors and Affiliations

  1. Department of Molecular Virology Faculty of Medicine, Hebrew University of Jerusalem, POB 1172, Jerusalem, Israel

    Yechiel Becker

  2. Institut für Medizinische Virologie, der Universität Heidelberg, Im Neuenheimer Feld 324, W-6900, Heidelberg, Germany

    Gholamreza Darai

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© 1992 Springer-Verlag Berlin Heidelberg

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Clewley, J.P. (1992). Polymerase Chain Reaction Diagnosis of Human Parvovirus B19. In: Becker, Y., Darai, G. (eds) Diagnosis of Human Viruses by Polymerase Chain Reaction Technology. Frontiers of Virology, vol 1. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-84766-0_22

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  • DOI: https://doi.org/10.1007/978-3-642-84766-0_22

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