Summary
The results of two studies involving 54 children born to HIV-1 seropositive women are reported. In the first one, I tested 24 children by PCR and viral isolation from peripheral blood mononuclear cells: Results were concordant in 96% of the cases, showing that PCR is a valid alternative to viral culture for the diagnosis of perinatal HIV-1 infection. In the second study, I tested prospectively 30 newborns at birth, 4–9 weeks, and 6–9 months: diagnosis of HIV infection by PCR was possible at birth for 3 infants compared with 12 one month later. No changes in the results were observed between the second and third testing. I conclude that, in most cases, PCR will not detect the presence of HIV-1 proviral DNA at birth, but it is a powerful technique that can be used for the diagnosis of perinatal HIV-1 infection from the second month of life.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Amadori A, De Rossi A, Chieco-Blanchi L et al. (1990) Diagnosis of human immunodeficiency virus 1 infection in infants: in vitro production of virus-specific antibody in lymphocytes. Pediatr Infect Dis J 9:26–30
Blanche S, Rouzioux C, Guihard-Moscato ML et al. (1989) A prospective study of infants born to women seropositive for human immunodeficiency virus type 1. N Engl J Med 320:1643–1648
Borkowsky W, Krasinski K, Paul D et al. (1989) Human immunodeficiency virus type 1 antigenemia in children. J Pediatr 114:942–945
Chadwick EG, Yogev R, Kwok S et al. (1989) Enzymatic amplification of the human immunodeficiency virus in peripheral blood mononuclear cells from pédiatric patients. J Infect Dis 160:954–959
Edwards JR, Ulrich PP, Weintrub PS et al. (1989) Polymerase chain reaction compared with concurrent viral cultures for rapid identification of human immunodeficiency virus infection among high-risk infants and children. J Pediatr 115:200–203
European Collaborative Study (1991) Children born to women with HIV-1 infection: natural history and risk of transmission. Lancet 1:253–260
Higuchi R (1989) Rapid, efficient DNA extraction for PCR from cells or blood. Amplifications 2:1–3
Hoff R, Berardi VP, Weiblen BJ et al. (1988) Seroprevalence of human immunodeficiency virus among childbearing women. N Engl J Med 318:325–330
Hutto C, Parks WP, Lai S et al. (1991) A hospital-based prospective study of perinatal infection with human immunodeficiency virus type 1. J Pediatr 118:347–353
Jackson JB, Coombs RW, Sannerud K et al. (1988) Rapid and sensitive viral culture method for human immunodeficiency virus type 1. J Clin Microbiol 26:1416–1418
Krivine A, Yakudima A, LeMay M et al. (1990) A comparative study of virus isolation, polymerase chain reaction, and antigen detection in children of mothers infected with human immunodeficiency virus. J Pediatr 116:372–376
Krivine A, Firtion G, Cao L et al. (1992) HIV replication during the first weeks of life. Lancet 339:1187–1189
Laure F, Courgnaud V, Rouzioux C et al. (1988) Detection of HIV-1 DNA in infants and children by means of the polymerase chain reaction. Lancet 2:538–540
McKinney RE, Maha M, Connor EM et al. (1991) A multicenter trial of oral zidovudine in children with advanced human immunodeficiency virus disease. N Engl J Med 324:1018–1025
Ou CY, Kwok S, Mitchell SW et al. (1988) DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells. Science 239:295–297
Rogers MF, Ou CY, Rayfield M et al. (1989) Use of the polymerase chain reaction for early detection of the proviral sequences of human immunodeficiency virus in infants born to seropositive mothers. N Engl J Med 320:1649–1654
Ryder RW, Nsa W, Hassig SE et al. (1989) Perinatal transmission of the human immunodeficiency virus type 1 to infants of seropositive women in Zaire. N Engl J Med 320:1637–1642
Saiki RK, Gelfand DH, Stoffel S et al. (1988) Primer directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:287–291
Scott GB, Hutto C, Makuch RW et al. (1989) Survival in children with perinatally acquired immunodeficiency virus infection type 1. N Engl J Med 321:1791–1796
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1992 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Krivine, A. (1992). Human Immunodeficiency Virus Type 1 (HIV-1) Detection by Polymerase Chain Reaction in Children of Infected Mothers. In: Becker, Y., Darai, G. (eds) Diagnosis of Human Viruses by Polymerase Chain Reaction Technology. Frontiers of Virology, vol 1. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-84766-0_2
Download citation
DOI: https://doi.org/10.1007/978-3-642-84766-0_2
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-84768-4
Online ISBN: 978-3-642-84766-0
eBook Packages: Springer Book Archive