Abstract
Vacuoles are the largest compartment in yeast cells, occupying about 25% of the total cell volumes. Vacuoles contain mainly low molecular weight solutes and ions, but quite small amount of proteins. Recent studies revealed that vacuoles are not inert organelles as previously thought, but play important roles on maintaining homeostasis of cytosol in many respects. Last 15 years we have been making efforts to understand the structure and function of vacuoles in yeast, Saccharomyces cerevisiae, by taking several different approaches, as described below.
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References
Achstetter T, Wolf DH (1985) Proteinases, proteolysis and biological control in the yeast Saccharomyces cerevisiae. Yeast 1:139–157.
Anraku Y, Umemoto N, Hirata R, Wada Y (1989) Structure and function of the yeast vacuolar membrane proton ATPase. J. Bioenerg. Biomembr. 21:589–603.
Henomatsu N, Yoshimori T, Moriyama Y, Futai M, Tashiro Y (1990) Effect of Bafilomycin A1 on intracellular distribution of prolactin in the GH3 rat pituitary tumor cells. Cell Struct. Funct. 15:447.
Jones EW (1984) The synthesis and function of proteinases in Saccharomyces cerevisiae: genetic approaches. Annu. Rev. Genet. 18:233–270.
Kakinuma Y, Ohsumi Y, Anraku Y (1981)Properties of H+-translocating ATPase in vacuolar membrane of Saccharomyces cerevisiae. J. Biol. Chem. 256:10859–10863.
Kitamoto K, Yoshizawa K, Ohsumi Y, Anraku Y (1988) Dynamic aspects of vacuolar and cytosolic amino acid pools of Saccharomyces cerevisiae. J. Bacteriol. 170:2683–2686.
Kitamoto K, Yoshizawa K, Ohsumi Y, Anraku Y (1988) Mutants of Saccharomyces cerevisiae with defective vacuolar function. J. Bacteriol. 170:2687–2691.
Klionsky DJ, Herman PK, Emr SD (1990) The fungal vacuole: Composition, function, and biogenesis. Microbiol. Rev. 54:266–292.
Klionsky DJ, Banta LM, Emr SD (1988) Intracellular sorting and processing of a yeast vacuolar hydrolase: proteinase A propeptide contains vacuolar targeting information. Mol. Cell. Biol. 8:2105–2116.
Matile P (1975) The lytic compartment of plant cells. Cell Biology Monographs, Vol. 1, Springer-Verlag, Wien, New York.
Ohsumi Y, Anraku Y (1981) Active transport of basic amino acids driven by a proton motive force in vacuolar membrane vesicles of Saccharomyces cerevisiae. J. Biol. Chem. 256:2079–2082.
Ohsumi Y, Anraku Y (1983) Calcium transport driven by a proton motive force in vacuolar membrane vesicles of Saccharomyces cerevisiae. J. Biol. Chem. 258:5614–5617.
Ohsumi Y, Anraku Y (1985) Specific induction of Ca2+ transport activity in MATa cells of Saccharomyces cerevisiae by a mating pheromone a factor. J. Biol. Chem. 269:10482–10486.
Ohsumi Y, Kitamoto K, Anraku Y (1988) Changes induced in thepermeability barrier of the yeast plasma membrane by cupric ion. J. Bacteriol. 170:2676–2682.
Rothman JH, Yamashiro CT, Raymond CK, Kane PM, Stevens TH (1989) Acidification of the lysosome-like vacuole and vacuolar H+-ATPase are deficient in two yeast mutants that fail to sort vacuolar proteins. J. Cell. Biol. 109:93–100.
Sato T, Ohsumi Y, Anraku, Y (1984) Substrate specificities of active transport systems for amino acids in vacuolar membrane vesicles of Saccharomyces cerevisiae. J. Biol. Chem. 259:11505–11508.
Uchida E, Ohsumi Y, Anraku Y (1985) Purification and properties of H+-translocating, Mg2+-adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae. J. Biol. Chem. 260:1090–1095.
Uchida E, Ohsumi Y, Anraku Y (1988) Characterization and function of catalytic subunit a of H+-translocating adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae. J. Biol. Chem. 263: 45–51.
Wada Y, Ohsumi Y, Tanifuji M, Kasai M, Anraku Y (1987) Vacuolar ion channel of the yeast, Saccharomyces cerevisiae. J. Biol. Chem. 262:17260–17263.
Wiemken A, Schellenberg M, Urech K (1979) Vacuoles: the sole compartments of digestive enzymes in yeast (Saccharomyces cerevisiae. Arch. Microbiol. 123:23–35.
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© 1993 Springer-Verlag Berlin Heidelberg
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Ohsumi, Y. (1993). Physiological functions of vacuoles in yeast: Mechanism of sequestration of metabolites and proteins into vacuoles. In: Maresca, B., Kobayashi, G.S., Yamaguchi, H. (eds) Molecular Biology and its Application to Medical Mycology. NATO ASI Series, vol 69. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-84625-0_6
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DOI: https://doi.org/10.1007/978-3-642-84625-0_6
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