Skip to main content
SpringerLink
Log in

The complement C3D-binding receptor (CR2) of Candida albicans: Cloning and sequence analysis of a gene fragment homologous with a human CR2 cDNA clone

  • Conference paper
Molecular Biology and its Application to Medical Mycology

Part of the book series: NATO ASI Series ((ASIH,volume 69))

Abstract

We have previously purified and characterized a complement receptor-like protein (CR2) from Candida albicans, which recognizes the C3 complement conversion product C3d. The Candida CR2 is a 60 kD mannoprotein while the native protein is approximately 240 kD. The CR2 is found on the cell surface of germinating blastoconidia, hyphae and pseudohyphae but is not observed on the surface of blastoconidia. CR2 is expressed during infection in mice with the organism as determined by immunoelectron microscopy and lymphocyte responses to the purified CR2. Recently, we have expanded our studies to include the molecular characterization of the CR2 gene. Southern blot analysis of EcoR1-digested and Hind III-digested C. albicans genomic DNA was pursued using a cDNA clone of the human B-cell CR2 gene as a probe. Homology to four Eco R1-generated fragments of 11, 8, 3.2, and 1.3 kb and two Hind III-generated fragments of 9.5 and 0.7 kb was demonstrated. The hybridization patterns also suggested that the C. albicans CR2 gene was most likely a single copy gene. The 3.2 kb Eco R1 fragment has been isolated by plaque hybridization from a partial genomic library-constructed in λgt11 using the same human CR2 to detect recombinants. Cross-hybridization analysis of the fragment localized homology to a 900 kb region. Sequence analysis of the 900 kb fragment has been completed.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Similar content being viewed by others

References

  • Bouchara J, Tronchin G, Annaix V, Robert R, Senet JM (1990) Presence of laminin receptors on Candida albicans. Infect. Immun. 58: 48–54

    PubMed  CAS  Google Scholar 

  • Calderone RA, Braun P (1991) Adherence and receptor relationships of Candida albicans. Microbiol. Rev. 55: 1–20.

    PubMed  CAS  Google Scholar 

  • Calderone RA, Linehan L, Wadsworth E, Sandberg AL (1988) Identification of C3d receptors on Candida albicans. Infect. Immun. 56: 252–258.

    PubMed  CAS  Google Scholar 

  • Calderone RA, Cihlar RL, Lee D, Hoberg K, Scheid WM (1985) Yeast adhesion in the pathogenesis of endocarditis due to Candida albicans studies with adherence-negative mutants. J. Infect. Dis. 152: 710–715.

    Article  PubMed  CAS  Google Scholar 

  • Critchley IA, Douglas LJ (1987) Role of glycosides as epithelial cell receptors for Candida albicans. J. Gen. Microbiol. 133: 637–643.

    PubMed  CAS  Google Scholar 

  • Critchley IA, Douglas LJ (1987) Isolation and partial characterization of an adhesin from Candida albicans. J. Gen. Microbiol. 133: 629–636.

    PubMed  CAS  Google Scholar 

  • Douglas LJ (1987) Adhesion to epithelial surfaces. In: O’Leary W (ed) Crit Rev in Microbiol, vol xv. CRC Press, Inc, Boca Raton FL, p 27.

    Google Scholar 

  • Douglas LJ (1991) Adhesion of Candida albicans to host surfaces. In: Tumbay E (ed) Proceedings of FEMS symposium on Candida and candidamycosis, Plenum Press, New York, p 43–48.

    Google Scholar 

  • Edwards JE, Gaither TA, O’Shea JJ, Rotrosen D, Lawley TJ, Wright SA, Frank FF, Green I (1986) Expression of specific binding sites on Candida with functional and antigenic characteristics of human complement receptors. J. Immunol. 137: 3577–3583.

    PubMed  CAS  Google Scholar 

  • Eigentler A, Schulz TF, Larcher C, Breitwieser EM, Myones B, Petzer AL, Dierich MP (1989) iC3b-binding protein on Candida albicans: temperature-dependent expression and relationship to human complement receptor type 3. Infect. Immun. 57: 616–622.

    PubMed  CAS  Google Scholar 

  • Fingeroth J (1991) Comparative structure and evolution of murine CR2. The homology of the human C3d/EBV Receptor (CD 21). J. Immunol. 144: 3458–3467

    Google Scholar 

  • Frey CL, Barone JM, Drutz D (1990) The role of the Candida albicansreceptor in fungal adherence to endothelial cells. Abstracts of the American Society for Microbiology, F101.

    Google Scholar 

  • Fukayama M, Calderone RA (1991) Adherence of cell surface mutants of Candida albicans to buccal epithelial cells and analyses of the cell surface proteins of the mutants. Infect. Immun. 59: 1341–1345.

    PubMed  CAS  Google Scholar 

  • Fukayama M, Wadsworth EA, Calderone RA (1992) Expression of the C3d-binding protein (CR2) from Candida albicans during experimental candidiasis as measured by lymphoproliferation. Infect. Immun. 60: in press.

    Google Scholar 

  • Gilmore BJ, Resinas EM, Lorenz JS, Hostetter MK (1988) An iC3b receptor on Candida albicans: structure, function and correlates for pathogenicity. J. Infect. Dis. 157: 38–46.

    Article  PubMed  CAS  Google Scholar 

  • Gustafson KS, Vercelloti GM, Lorenz JS, Hostetter MK (1991) Molecular mimicry in Candida albicans. Role of an integrin in adhesion of the yeast to human endothelium. J. Clin. Invest. 87: 1896–1902.

    CAS  Google Scholar 

  • Heidenreich F, Dierich MP (1985) Candida albicans and Candida stellatoidea, in contrast to other Candida species, bind iC3b and C3d but not C3b. Infect. Immun. 50: 598–600.

    PubMed  CAS  Google Scholar 

  • Hoberg K, Cihlar RL, Calderone RA (1986) Characterization of cerulenin-resistant mutants of Candida albicans. Infect. Immun. 51: 102–109.

    PubMed  CAS  Google Scholar 

  • Hostetter MK, Lorenz JS, Preus L, Kendrick KE (1990) The iC3b receptor of Candida albicans: subcellular localization and modulation of receptor expression by glucose. J. Infect. Dis. 161: 761–768.

    Article  PubMed  CAS  Google Scholar 

  • Li RK, Cutler JE (1991) A cell surface/plasma membrane antigen of Candida albicans. J. Gen. Microbiol. 137: 455–464.

    PubMed  CAS  Google Scholar 

  • Klotz SA (1990) Adherence of Candida albicans to components of the subendothelial extracellular matrix. FEMS Microbiol. Lett. 68: 249–254.

    CAS  Google Scholar 

  • Klotz SA, Smith RL (1990) Candida albicans adherence to subendothelial ECM is inhibited by arginine-glycine-aspartic acid peptides. Clin Res, 38: 13A, AFCR Immunol. Rheumatol. Conf. New Orleans, LA.

    Google Scholar 

  • Linehan L, Wadsworth E, Calderone RA (1988) Candida albicansC3d receptor, isolated by using a monoclonal antibody. Infect. Immun. 56: 1981–1986.

    PubMed  CAS  Google Scholar 

  • Marcantonio E, Hynes RO (1988) Antibodies to the conserved cytoplasmic domain of the integrin 1 subunit react with proteins in vertebrates, invertebrates and fungi. J. Cell. Biol. 106: 1765–1772.

    Article  PubMed  CAS  Google Scholar 

  • Mayer CL, Diamond RD, Edwards JE (1990) Recognition of binding sites on Candida albicans by monoclonal antibodies to human leukocyte antigens. Infect. Immun. 58: 3765–3769.

    PubMed  CAS  Google Scholar 

  • Moore MM, Cooper NR, Tack BF (1987) Nemerow GR Molecular cloning of the cDNA encoding the Epstein-Barr virus-C3d receptor (complement receptor type 2) of human B-lymphocytes. Proc. Natl. Acad. Sci. USA, 84: 9194–9198.

    Article  PubMed  CAS  Google Scholar 

  • Ollert MK, Wadsworth E, Calderone RA (1990) Reduced expression of the functionally active complement receptor for iC3b but not for C3d on an avirulent mutant of Candida albicans. Infect. Immun. 58: 909–913.

    PubMed  CAS  Google Scholar 

  • Ross GD (1986) Opsonization and membrane complement receptors. In: Ross GD (ed) Immunobiology of the complement system. Academic Press, Inc. New York p.87.

    Google Scholar 

  • Ruchel R (1981) Properties of a purified proteinase from the yeast Candida albicans. Biochim. Biophys. Acta, 659: 99–113.

    PubMed  CAS  Google Scholar 

  • Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74: 5463–5467.

    Article  PubMed  CAS  Google Scholar 

  • Saxena A, Calderone RA (1990) Purification and characterization of the extracellular C3d-binding protein of Candida albicans. Infect. Immun. 58: 309–314.

    PubMed  CAS  Google Scholar 

  • Shepherd MG (1987) Cell envelope of Candida albicans. In: O’Leary WO (ed) CRC Critical Rev in Microbiol, Boca Raton, FL, 15: 7–27.

    Google Scholar 

  • Skerl KG, Calderone RA, Segal E, Sreevalsan T, Scheid WM (1983) In vitro binding of Candida albicans yeast cells to human fibronectin. Canad. J. Microbiol. 30: 221–227.

    Article  Google Scholar 

  • Stoolman LM (1989) Adhesion molecules controlling lymphocyte migration. Cell, 56: 907–910.

    Article  PubMed  CAS  Google Scholar 

  • Tronchin G, Bouchara JP, Robert R (1989) Dynamic changes of the cell wall surface of Candida albicans associated with germination and adherence. Eur. J. Cell. Biol. 50: 285–290.

    PubMed  CAS  Google Scholar 

  • Whelan WL, Delga JM, Wadsworth E, Walsh TJ, Kwon-Chung KJ, Calderone RA, Lipke P (1990) Isolation and characterization of cell surface mutants of Candida albicans. Infect. Immun. 58: 1552–1557.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Microbiology, Georgetown University School of Medicine, 3900 Reservoir Rd., NW., Washington, DC, 20007, USA

    Richard A. Calderone, Susan Southard & Cynthia Hale

Authors
  1. Richard A. Calderone
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Susan Southard
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Cynthia Hale
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. International Institute of Genetics and Biophysics, Via Marconi 12, 80125, Naples, Italy

    Bruno Maresca

  2. Division of Infectious Diseases St. Louis, Washington University School of Medicine, Missouri, 63110, USA

    George S. Kobayashi

  3. Research Center for Medical Mycology, Teikyo University School of Medicine, 359 Otsuka, Hachioji-shi, Tokyo, 192-03, Japan

    Hideyo Yamaguchi

Rights and permissions

Reprints and permissions

Copyright information

© 1993 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Calderone, R.A., Southard, S., Hale, C. (1993). The complement C3D-binding receptor (CR2) of Candida albicans: Cloning and sequence analysis of a gene fragment homologous with a human CR2 cDNA clone. In: Maresca, B., Kobayashi, G.S., Yamaguchi, H. (eds) Molecular Biology and its Application to Medical Mycology. NATO ASI Series, vol 69. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-84625-0_4

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-84625-0_4

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-84627-4

  • Online ISBN: 978-3-642-84625-0

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation