Abstract
Filamentous fungi such as Aspergillus and Penicillium are extensively used in industries which produce alcohol, organic acid, and antibiotics. So far breedings of these useful fungi have been mainly achieved by methods of mutagenesis. Recently, the application of recombinant DNA technology to filamentous fungi has allowed strain improvement. Since Aspergilli produce a large amount of enzymes to decompose starch and proteins to sugars and amino acids, respectively, it is possible that these fungi serve useful secretion systems with strong expression promoter for efficient productions of heterologous proteins. Among the filamentous fungi, Aspergillus nidulans and Neurospora crassa have been most extensively investigated in molecular genetics. Based on the knowledge of these fungi, the molecular genetic systems of several industrial species, including Aspergillus niger and Aspergillus oryzae, have been developed and allowed strain improvement by increasing the number of genes encoding enzymes of importance.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Beri RK and Turner G (1987) Transformation of Penicillium chrysogenum using A. nidulans amdS gene as a dominant selective marker. Curr. Genet. 11: 639–641.
Christensen T, Woeldike H, Boel E, Mortensen SB, Hjortshoej K, Thim L and Hansen MT (1988) High level expression of recombinant genes in Aspergillus oryzae. Biol.Tech. 6: 1419–1422.
de Ruiter-Jacobs YMJT, Broekhuijsen M, Unkles SE, Campbell EI, Kinghorn JR, Contreras R, Pouwels PH and van den Hondel CAMJJ (1989). A gene transfer system based on the homologous pyrG gene and efficient expression of bacterial genes in Aspergillus oryzae. Curr. Genet. 16: 159–163
Erratt JA, Douglas P, Morrannelli F and Seligy VL (1984) The induction of α-amylase by starch in Aspergillus oryzae. evidence for controlled mRNA expression. Can. J. Biochem. Cell Biol. 62: 678–690.
Gems D, Johnstone IL and Clutterbuck AJ (1991) An autonomously replicating plasmid transforms Aspergillus nidulans at high frequency. Gene 98: 61–67.
Gines MJ, Dove MJ and Seligy VL (1989) Aspergillus oryzae has two nearly identical Taka-amylase genes, each containing eight introns. Gene 79: 107–117.
Gomi K, Iimura Y and Hara S (1987) Integrative transformation of Aspergillus oryzae with a plasmid containing the Aspergillus nidulans argB gene. Agric. Biol. Chem. 51: 2549–2555.
Gomi K, Kitamoto K and Kumagai C (1991) Cloning and molecular characterization of the acetamidase-encoding gene amdS from Aspergillus oryzae. Gene 108: 91–98
Gurr S, Unkles SE and Kinghorn JR (1987) The structure and organization of nuclear genes of filamentous fungi. In Gene Structure in Eukaryotic Microbes (JR Kinghorn, Ed.) pp.93–139. IRL Press, Oxford.
Hahm YT and Batt CA (1988) Genetic transformation of argB mutant of Aspergillus oryzae. App. Env. Microbiol. 54: 1610–1611
Hata Y, Tsuchiya K, Kitamoto K, Gomi K, Kumagai C, Tamura G and Hara S (1991) Nucleotide sequence and expression of the glucoamylase-encoding gene (glaA) from Aspergillus oryzae. Gene 108: 145–150
Iimura Y, Gomi K, Uzu H and Hara S (1987) Transformation of Aspergillus oryzae through the complementation of methionine auxotrophic mutation. Agric. Biol. Chem. 51: 323–328.
Kelly JM and Hynes MJ (1985) Transformation of Aspergillus niger by the amdS gene Aspergillus nidulans. EMBO J, 4: 475–479.
Kelly JM and Hynes MJ (1987) Multiple copies of the amdS gene of Aspergillus nidulans causes titration of trans-acting regulation proteins. Curr. Genet. 12: 21–31.
Mattern IE, Unkles SE, Kinghorn JR, Pouwels PH and Van den Hondel CAMJJ (1987) Transformation of Aspergillus oryzae using the A. niger pyrG gene. Mol. Gen. Genet. 210:460–461.
Penttila M, Nevalainen H, Ratto M, Salminen E and Knowles J (1987) A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei. Gene 61: 155–163
Shibuya I, Gomi K, Iimura Y, Takahashi K, Tamura G and Hara S (1990) Molecular cloning of the glucoamylase gene of Aspergillus shirousamii and its expression in Aspergillus oryzae. Agric. Biol. Chem. 54: 1905–1914.
Tada S, Iimura Y, Gomi K, Takahashi K, Hara S and Yoshizawa K (1989) Cloning and nucleotide sequence of the genomic Taka-amylase A gene of Aspergillus oryzae. Agric. Biol. Chem. 53: 593–599.
Tada, S., Gomi, K., Kitamoto, K., Kumagai, C, Tamura, G. and Hara, S. (1991) Identification of the promoter region of the Taka-amylase A gene required for starch induction. Agric. Biol. Chem. 55: 1939–1941.
Toda H, Kondo K and Narita K (1982) The complete amino acid sequence of Taka-amylase A. Proc. Jap. Acad. 58B: 208–212.
Unkles SE, Campbell EI, de Ruiter-Jacobs YMJT, Broekhuijsen M, Macro JA, Carrez D, Contreras R, Van den Hondel CAMJJ and Kinghorn JR (1989) The development of a homologous transformation system for Aspergillus oryzae based on the nitrate assimilation pathway: A convenient and general selection system for filamentous fungal transformation. Mol. Gen. Genet. 218: 99–104.
Van Heeswijck R (1986) Autonomous replication of plasmids in Mucor transformants. Carlsberg Res. Comm. 51: 433–443.
Ward M, Kodama KH and Wilson LJ (1989) Transformation of Aspergillus awamori and A.niger by electroporation. Exp. Mycol. 13: 289–293.
Wirsel S, Lachmund A, Wildhardt G and Ruttkowski E (1989) Three α-amylase genes of Aspergillus oryzae exhibit identical intron-exon organization. Mol. Microbiol. 3: 3–14.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1993 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Iimura, Y., Gomi, K. (1993). Molecular breeding in filamentous fungi with emphasis on Aspergilli . In: Maresca, B., Kobayashi, G.S., Yamaguchi, H. (eds) Molecular Biology and its Application to Medical Mycology. NATO ASI Series, vol 69. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-84625-0_11
Download citation
DOI: https://doi.org/10.1007/978-3-642-84625-0_11
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-84627-4
Online ISBN: 978-3-642-84625-0
eBook Packages: Springer Book Archive