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High-Performance Liquid Chromatography of Catecholamines and their Metabolites in Biological Material

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High-Performance Liquid Chromatography in Endocrinology

Part of the book series: Monographs on Endocrinology ((ENDOCRINOLOGY,volume 30))

Abstract

The use of high-performance liquid chromatography (HPLC) as an analytical tool for the life sciences has become increasingly important and the techniques and instrumentation involved are continuously developing to higher levels of sophistication. One result of this rapid progress is that a user of HPLC for a certain bioanalytical purpose is often faced with a great problem of selecting from the overwhelming menu offered in the field with respect to choice of column type, mobile phase, elution technique, detector system, degree of computerization etc. To this are then added the particular problems associated with the pre-chromatographic work, i.e. the extraction, prepurification and concentration steps often necessary to produce a sample suitable for injection into an HPLC-system.

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  1. S. Allenmark
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Editors and Affiliations

  1. The London Hospital Medical College Department of Chemical Pathology, University of London, Turner Street, London, E1 2AD, GB

    H. L. J. Makin Dr.

  2. Biotech Instruments Ltd., 183 Camford Way, Bedfordshire, LU 3 3AN, GB

    R. Newton Dr.

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Allenmark, S. (1988). High-Performance Liquid Chromatography of Catecholamines and their Metabolites in Biological Material. In: Makin, H.L.J., Newton, R. (eds) High-Performance Liquid Chromatography in Endocrinology. Monographs on Endocrinology, vol 30. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-83467-7_2

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  • DOI: https://doi.org/10.1007/978-3-642-83467-7_2

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