Abstract
Northern blotting analysis is the standard procedure used to confirm that a cDNA band isolated from a differential display gel is a true positive and represents a gene differentially expressed in the system under study. Slot blot analysis may also be used for preliminary screening of a large number of putative positive cDNAs, but results need to be reconfirmed on Northern blot.
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© 1998 Springer-Verlag Berlin Heidelberg
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Colonna-Romano, S., Leone, A., Maresca, B. (1998). Confirmation of Differential Expression of Cloned cDNA Fragments. In: Differential-Display Reverse Transcription-PCR (DDRT-PCR). Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-80454-0_9
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DOI: https://doi.org/10.1007/978-3-642-80454-0_9
Publisher Name: Springer, Berlin, Heidelberg
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