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Electron Beam Radiation Damage to Organic and Biological Cryospecimens

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Cryotechniques in Biological Electron Microscopy

Abstract

Radiation damage, i.e. the introduction of structural (e.g. loss of crystallinity) or compositional (e.g. mass loss) changes by the electron beam, has been an inevitable and serious factor in limiting the amount of microstructural information that can be collected from an electron microscope specimen. Although radiation damage is always present because it is part of the electron beam/specimen interaction that produces the image, it may be reduced or minimized, if it and the physical factors that affect it are well understood.

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Author information

Authors and Affiliations

  1. Department of Chemical Engineering, Technion — Israel Institute of Technology, Haifa, 32000, Israel

    Yeshayahu Talmon

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  1. Yeshayahu Talmon
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Editors and Affiliations

  1. Max-Planck-Institut für Verhaltensphysiologie, D-8131, Seewiesen, Germany

    Rudolf Alexander Steinbrecht

  2. Max-Planck-Institut für Systemphysiologie, Rheinlanddamm 201, D-4600, Dortmund, Germany

    Karl Zierold

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© 1987 Springer-Verlag Berlin Heidelberg

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Talmon, Y. (1987). Electron Beam Radiation Damage to Organic and Biological Cryospecimens. In: Steinbrecht, R.A., Zierold, K. (eds) Cryotechniques in Biological Electron Microscopy. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-72815-0_3

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  • DOI: https://doi.org/10.1007/978-3-642-72815-0_3

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-72817-4

  • Online ISBN: 978-3-642-72815-0

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