Abstract
L-929 cells derive from a mouse connective tissue (Earle 1943). They secrete a neurotrophic factor which is immunologically and biochemically similar to the β-NGF found in mouse submaxillary glands (Oger et al. 1974; Brachet and Dicou 1984). Hybridizations with a β-NGF cDNA probe (Scott et al. 1983) have recently shown that L cells contain a β-NGF transcript identical in size to that produced by the submaxillary gland, and that the β-NGF locus of the genomic DNA is not rearranged, as evidenced by cleavage with some restriction enzymes (Wion et al. 1985). L cells, therefore, constitute a valuable model system suited for the study of the gene expression of the factor. The present work deals with a preliminary characterization of extracellular effector molecules which influence this expression. Extracellular concentrations of β-NGF were correlated with intracellular levels of the β-NGF mRNA in cells cultured in the presence or absence of serum, or exposed to testosterone, T3 or T4, which are known to enhance the β-NGF levels in the mouse submaxillary gland (Levi Montalcini and Angeletti 1968; Aloe and Levi Montalcini 1980).
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© 1986 Springer-Verlag Berlin Heidelberg
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Houlgatte, R., Wion, D., Barrand, P., Dicou, E., Brachet, P. (1986). Serum Influences β-NGF Gene Expression in Mouse L Cells. In: Montalcini, R.L., Calissano, P., Kandel, E.R., Maggi, A. (eds) Molecular Aspects of Neurobiology. Proceedings in Life Sciences. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70690-5_8
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DOI: https://doi.org/10.1007/978-3-642-70690-5_8
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