Skip to main content
SpringerLink
Log in

Calcium Currents in Mammalian Central Neurones

  • Conference paper
Cardiovascular Effects of Dihydropyridine-Type Calcium Antagonists and Agonists

Part of the book series: Bayer-Symposium ((BAYER-SYMP,volume 9))

Abstract

Voltage-activated Ca-currents (ICa) are present in most neurones (Hagiwara and Byerly 1981; Kostyuk 1981). This article concerns the Ca-currents observed in mammalian central neurones. Their properties have been inferred from a variety of Ca-dependent voltage-transients (reviewed by Llinas and Walton 1980); and Ca-currents have been recorded directly in voltage-clamp experiments on spinal motoneurones (Schwindt and Crill 1980a,b), in hippocampal pyramidal cells (Johnston et al. 1980; Brown and Griffith 1983 b; Halliwell 1983) and in olfactory cortex neurons (A. Constanti and M. Galvan, unpublished observations).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Similar content being viewed by others

References

  • Adams PR, Brown DA, Halliwell JV (1981) Cholinergic regulation of M-current in guinea-pig hippocampal neurones. J Physiol Lond 317:29–30.

    Google Scholar 

  • Aldenhoff JB, Gruol DL, Rivier J, Vale W, Siggins GR (1983) Corticotrophin releasing factor decreases postburst hyperpolarizations and excites hippocampal neurons. Science NY 221:875–877.

    Article  CAS  Google Scholar 

  • Barrett JF, Barrett JN, Crill WE (1980) Voltage-sensitive outward currents in cat motoneurones. J Physiol Lond 304:251–276.

    PubMed  CAS  Google Scholar 

  • Bellemann P, Schade A, Towart R (1983) Dihydropyridine receptor in rat brain labelled with 3H nimodipine Proc Natl Acad Sci USA 80:2356–2360.

    Article  PubMed  CAS  Google Scholar 

  • Benardo LS, Masukawa LM, Prince DA (1982) Electrophysiology of isolated hippocampal pyramidal dendrites. J Neurosci 2:1614–1622.

    PubMed  CAS  Google Scholar 

  • Benardo LS, Prince DA (1981) Acetylcholine induced modulation of hippocampal pyramidal neurons. Brain Res 211:227–234.

    Article  PubMed  CAS  Google Scholar 

  • Bormann J, Sakmann B, Seifert W (1983) Isolation of GABA-activated single-channel Cl- currents in the soma membrane of rat hippocampal neurones. J Physiol, Lond, 341:9-10P.

    Google Scholar 

  • Brown DA, Adams PR (1980) Muscarinic suppression of a noval voltage-sensitive K-current in voltage-clamped hippocampal cell somata. Nature, Lond, 183:673–676.

    Article  Google Scholar 

  • Brown DA, Griffith WH (1983a) Calcium-activated outward current in voltage-clamped hippocampal neurones of the guinea-pig. J Physiol, Lond, 337:287–301.

    PubMed  CAS  Google Scholar 

  • Brown DA, Griffith WH (1983) Persistent slow inward current in voltage-clamped hippocampal neurones of the guinea-pig. J Physiol, Lond, 337:303–320.

    PubMed  CAS  Google Scholar 

  • Brown DA, Constanti A, Docherty RJ, Galvan M, Gahwiler B, Halliwell JV (1984) Pharmacology of calcium currents in mammalian central neurons. Proc 9th Int. Pharmacol Congress (in press).

    Google Scholar 

  • Brown TH, Fricke RA, Perkel DH (1980) Passive electrical constants in three classes of hippocampal neurons. J Neurophysiol 46:812–827.

    Google Scholar 

  • Carbonne E, Lux HD (1984) A low voltage-activated, fully inactivating Ca-channel in vertebrate sensory neurones. Nature, Lond, 310:501–502.

    Article  Google Scholar 

  • Cherubini E, North RA (1984). Actions of γ-aminobutyric acid on neurones of guinea-pig myenteric plexus. Br J Pharmac 82:93–100.

    CAS  Google Scholar 

  • Cole AE, Nicoll RA (1984) The pharmacology of cholinergic excitatory responses in hippocampal pyramidal cells. Brain Res 305:283–290.

    Article  PubMed  CAS  Google Scholar 

  • Colquhoun D, Neher E, Reuter H, Stevens CF (1981) Inward current channels activated by intracellular Ca in cultured cardiac cells. Nature, Lond, 294:752–754.

    Article  CAS  Google Scholar 

  • Connor JA, Stevens CF (1971) Voltage-clamp studies of a transient outward membrane current in gastropod neural somata. J Physiol Lond 213:21–30.

    PubMed  CAS  Google Scholar 

  • Constanti A, Brown DA (1981) M-currents in voltage-clamped mammalian sympathetic neurones. Neurosci Lett 24:289–294.

    Article  PubMed  CAS  Google Scholar 

  • Cortes R, Supavilai P, Karobath M, Palacois JM (1983) The effect of lesions in the rat hippocampus suggest the association of calcium channel blocker binding sites with specific neuronal population. Neurosci Lett 42:249–254.

    Article  PubMed  CAS  Google Scholar 

  • Curtis BM, Catterall WA (1983) Solubilization of the calcium antagonist receptor from rat brain. J Biol Chem 258:7280–7283.

    PubMed  CAS  Google Scholar 

  • Desarmenien M, Feltz P, Occhipinti G, Santangelo F, Schlichter R (1984) Coexistence of GA-BAA and GABAB receptors on A and C primary afferents. Br J Pharmac 81:327–334.

    CAS  Google Scholar 

  • Deschenes M, Roy JP, Steriade M (1982) Thalamic bursting mechanism: an inward slow current revealed by a membrane hyperpolarization. Brain Res 239:289–293.

    Article  PubMed  CAS  Google Scholar 

  • Dodd J, Dingeldine R, Kelly JS (1980) The excitatory action of acetylcholine on hippocampal neurones of the guinea-pig and rat maintained in vitro. Brain Res 207:109–127.

    Article  Google Scholar 

  • Dolly J, Halliwell JV, Black JD, Williams RS, Pelchen-Matthews A, Breeze AL, Mehraban F, Othman IB, Black AR (1984) Botulinum neurotoxin and dendrotoxin as probes for studies on transmitter release. J Physiol Paris (in press).

    Google Scholar 

  • Dunlap K (1981) Two types of γ-aminobutyric acid receptor on embryonic sensory neurones. Br J Pharmac 74:579–585.

    CAS  Google Scholar 

  • Dunlap K, Fischbach GD (1981) Neuro transmitters decrease the calcium conductance activated by depolarization of embryonic chick sensory neurones. J Physiol, Lond 317:519–535.

    PubMed  CAS  Google Scholar 

  • Eckert R, Lux HD (1976) A voltage-sensitive persistent calcium conductance in neuronal somata of Helix. J Physiol, Lond 254:129–152.

    PubMed  CAS  Google Scholar 

  • Fox AP, Nowycky MC, Tsien RW (1984) Single Ca channel in dorsal root ganglion cells are shifted between modes of gating by the calcium agonist Bay K 8644. Neurosci Abstr 10:526.

    CAS  Google Scholar 

  • Freedman SB, Dawson G, Villereal ML, Miller RJ (1984) Identification and characterization of voltage-sensitive calcium channels in neuronal clonal lines. J Neurosci 4:1453–1467.

    PubMed  CAS  Google Scholar 

  • Gahwiler B (1981) Organotypic monolayer cultures of nervous tissue. J Neurosci Meth 4:329–342.

    Article  CAS  Google Scholar 

  • Gahwiler B, Brown DA (1985) GABAB-receptor activated K+-current in voltage-clamped CA3 pyramidal cells in hippocampal cultures. Proc Natl Acad Sci USA 82:1558–1562.

    Article  PubMed  CAS  Google Scholar 

  • Gahwiler BH, Dreifuss JJ (1982) Multiple actions of acetylcholine on hippocampal pyramidal cells in organotypic explant cultures. Neuroscience 7:1243–1256.

    Article  PubMed  CAS  Google Scholar 

  • Galvan M, Adams PR (1982) Control of calcium-current in rat sympathetic neurons by norepinephrine. Brain Res 244:135–144.

    Article  PubMed  CAS  Google Scholar 

  • Glossman H, Ferry DR, Lubbecke F, Mewes R, Hofmann F (1982) Calcium channels: direct identification with radioligand binding studies. Trends in Pharmacol Sci 3:431–437.

    Article  Google Scholar 

  • Gustafsson B, Galvan M, Grafe P, Wigstrom H (1982) A transient outward current in a mammalian central neurone blocked by 4-aminopyridine. Nature, Lond 299:252–254.

    Article  CAS  Google Scholar 

  • Haas HL, Konnerth A (1983) Histamine and noradrenaline decrease calcium-activated potassium conductance in hippocampal pyramidal cells. Nature, Lond 302:432–434.

    Article  CAS  Google Scholar 

  • Hablitz JJ, Johnston D (1981) Endogenous nature of spontaneous bursting in hippocampal pyramidal neurons. J Cell Molec Neurobiol 1:325–334.

    Article  CAS  Google Scholar 

  • Hagiwara S, Byerly L (1981) Calcium channel. Ann Rev Neurosci 4:69–125.

    Article  PubMed  CAS  Google Scholar 

  • Halliwell JV (1983) Caesium-loading reveals two distinct Ca-currents in voltage-clamped guineapig hippocampal neurones in vitro. J Physiol, Lond 341:10-11P.

    Google Scholar 

  • Halliwell JV, Adams PR (1982) Voltage-clamp analysis of muscarinic excitation in hippocampal neurons. Brain Res 250:71–92.

    Article  PubMed  CAS  Google Scholar 

  • Halliwell JV, Scholfield CN (1984 a) Adenosine-resistant Ca-currents in mammalian central nervous system. J Physiol, Lond 349:52P.

    Google Scholar 

  • Halliwell JV, Scholfield CN (1984 b) Somatically-recorded Ca-currents in guinea-pig hippocampal and olfactory cortex neurones are resistant to adenosine action. Neurosci Lett 50:13–18.

    Article  PubMed  CAS  Google Scholar 

  • Henon BK, McAfee DA (1983) Modulation of calcium currents by adenosine receptors on mammalian sympathetic neurones. In: Berne RM, Rall TW, Rabio R (eds) Regulatory function of adenosine. Martinus Nijhoff, The Hague, pp 455-466.

    Google Scholar 

  • Hotson JR, Prince DA (1981) Penicillin-and barium-induced epileptiform bursting in hippocampal neurons: actions on Ca2+ and K+-potentials. Ann Neurol 10:11–17.

    Article  PubMed  CAS  Google Scholar 

  • Johnston D (1981) Passive cable properties of hippocampal CA3 pyramidal neurons. Cell Mol Neurobiol 1:100–110.

    Article  Google Scholar 

  • Johnston D, Brown TH (1984) Mechanisms of neuronal burst generation. In: Schwartzkroin PA, Wheal HV (eds) Electrophysiology of epilepsy. Academic Press, London, pp 278–301.

    Google Scholar 

  • Johnston D, Hablitz JJ, Wilson WA (1980) Voltage-clamp discloses slow inward current in hippocampal burst-firing neurones. Nature, Lond 286:391–393.

    Article  CAS  Google Scholar 

  • Kandel ER, Spencer WA (1961) Electrophysiology of hippocampal neurons. II. After-potentials and repetitive firing. J Neurophysiol 24:243–259.

    PubMed  CAS  Google Scholar 

  • Kostyuk PG (1981) Calcium-channels in the neuronal membrane. Biochem Biophys Acta 650:128–150.

    PubMed  CAS  Google Scholar 

  • Lancaster B, Adams PR (1984) Single electrode voltage clamp of the slow AHP current in rat hippocampal pyramidal cells. Neurosci Abstr (in press).

    Google Scholar 

  • Lee KS, Tsien RW (1983) Mechanism of calcium channel blockade by verapamil, D 600, diltiazem and nitrendipine in single dialysed heart cells. Nature, Lond 302:790–794.

    Article  CAS  Google Scholar 

  • Llinas R, Sugimori M (1980) Electrophysiological properties of in vitro Purkinje cell dendrites in mammalian cerebellar slices. J Physiol, Lond 305:197–213.

    PubMed  CAS  Google Scholar 

  • Llinas R, Walton K (1980) Voltage-dependent calcium conductances in neurons. In: Cotman CW, Poste G, Nicolson GL (eds) The cell surface and neuronal function. Elsevier, North Holland, pp 87-118.

    Google Scholar 

  • Llinas R, Yarom Y (1981) Electrophysiology of mammalian inferior olivary neurones in vitro. Different types of voltage-dependent ionic conductances. J Physiol, Lond 315:549–567.

    CAS  Google Scholar 

  • Madison DV, Nicoll RA (1982) Noradrenaline blocks accommodation of pyramidal cell discharge in the hippocampus. Nature, Lond 299:636–638.

    Article  CAS  Google Scholar 

  • Madison DV, Nicoll RA (1984) Control of the repetitive discharge of CA1 pyramidal neurones in vitro. J Physiol Lond 354:318–331.

    Google Scholar 

  • Miller RJ, Freedman SB (1984) Are dihydropyridine binding sites voltage-sensitive calcium channels? Life Sci 34:1205–1221.

    Article  PubMed  CAS  Google Scholar 

  • Neher E (1971) The fast transient current components during voltage clamp on snail neurons. J Gen Physiol 58:36–53.

    Article  PubMed  CAS  Google Scholar 

  • Nelson MT, French RJ, Krueger BK (1984) Voltage-dependent calcium channels from brain incorporated into planar lipid bilayers. Nature, Lond 308:77–80.

    Article  CAS  Google Scholar 

  • Newberry NR, Nicoll RA (1984) Direct hyperpolarizing action of baclofen on hippocampal pyramidal cells. Nature, Lond 308:450–452.

    Article  CAS  Google Scholar 

  • Nowycky M, Fox AP, Tsien RW (1984) Two components of calcium channel currents in chick dorsal root ganglion cells. Biophys J, 45:36 a.

    Google Scholar 

  • Numann R, Wong RKS (1984) Voltage-clamp study on GABA response desensitziation in single pyramidal cells dissociated from the hippocampus of adult guinea-pigs. Neurosci, Lett 47:289–294.

    Article  CAS  Google Scholar 

  • Ogata N, Hori N, Katsuda N (1976) The correlation between extracellular potassium concentration and hippocampal epileptic activity in vitro. Brain Res 110:371–375.

    Article  PubMed  CAS  Google Scholar 

  • Owen DG, Segal M, Barker JL (1984) A Ca-dependent Cl--conductance is present in cultured mouse spinal neurones. Nature, Lond 311:567–570.

    Article  CAS  Google Scholar 

  • Prince DA (1978) Neurophysiology of epilepsy. Ann Rev Neurosci 1:395–415.

    Article  PubMed  CAS  Google Scholar 

  • Prince DA (1983) Mechanisms of epileptogenesis in brain-slice model systems. In: Ward AA Jr, Penny JK, Purpura D (eds) Epilepsy. Raven Press, New York, pp 29–52.

    Google Scholar 

  • Proctor WR, Dunwiddie TV (1983) Adenosine inhibits calcium spikes in hippocampal pyramidal neurons in vitro. Neurosci. Lett 35:197–201.

    Article  PubMed  CAS  Google Scholar 

  • Purpura DP, Prelevic S, Santini M (1968) Hyperpolarizing increase in membrane conductance in hippocampal neurons. Brain Res 7:310–312.

    Article  PubMed  CAS  Google Scholar 

  • Reuter H (1983) Calcium channel modulation by neurotransmitters, enzymes and drugs. Nature, Lond 301:569–574.

    Article  CAS  Google Scholar 

  • Schlichter R, Demeneix BA, Desarmenien M, Desaulles E, Loeffler JP, Feltz P (1984) Properties of the GABA receptors located on spinal primary afferent neurones and hypophyseal neuroendocrine cells of the rat. Neurosci Lett 47:257–263.

    Article  PubMed  CAS  Google Scholar 

  • Schwartzkroin PA (1980) Ionic and synaptic determinants of burst generation. In: Lockard JS, Ward Jr AA (eds) Epilepsy: A window to brain mechanisms. Raven press, New York, pp 83–95.

    Google Scholar 

  • Schwartzkroin PA, Prince DA (1980) Effect of TEA on hippocampal neurons Brain Res 185:169–181.

    Article  PubMed  CAS  Google Scholar 

  • Schwartzkroin PA, Slawsky M (1977) Probable calcium spikes in hippocampal neurones. Brain Res 135:157–161.

    Article  PubMed  CAS  Google Scholar 

  • Schwindt PC, Crill W (1980 a) Role of a persistent inward current in motoneuron bursting during spinal seizures. J Neurophysiol 43:1296–1318.

    PubMed  CAS  Google Scholar 

  • Schwindt PC, Crill W (1980 b) Properties of a persistent inward current in normal and TEA-injected motoneurons. J Neurophysiol 43:1700–1724.

    PubMed  CAS  Google Scholar 

  • Segal M, Barker JL (1984) Rat hippocampal neurones in culture: potassium current. J Neurophysiol 51:1409–1433.

    PubMed  CAS  Google Scholar 

  • Tillotson D (1979) Inactivation of Ca conductance dependent on entry of Ca ions in molluscan neurons. Proc Nat Acad Sci USA, 76:1497–1500.

    Article  PubMed  CAS  Google Scholar 

  • Toll L (1982) High-affinity binding and inhibition of calcium transport in a clonal cell line. J Biol Chem 257:13189–13192.

    PubMed  CAS  Google Scholar 

  • Traub RD (1982) Simulation of intrinsic bursting in CA3 hippocampal neurons. Neuroscience 7:1233–1242.

    Article  PubMed  CAS  Google Scholar 

  • Turner DA, Schwartzkroin PA (1980) Steady-state electrotonic analysis of intracellularly stained hippocampal neurons. J Neurophysiol 44:184–199.

    PubMed  CAS  Google Scholar 

  • Wong RKS, Prince DA (1978) Participation of calcium spikes sharing intrinsic burst firing in hippocampal neurons. Brain Res 159:385–390.

    Article  PubMed  CAS  Google Scholar 

  • Wong RKS, Prince DA (1979) Dendritic mechanisms underlying penicillin-induced epileptiform activity. Science, NY 204:1228–1230.

    Article  CAS  Google Scholar 

  • Wong RKS, Prince DA, Basbaum AI (1979) Intradendritic recordings from hippocampal neurons. Proc Natl Acad Sci 76:986–990.

    Article  PubMed  CAS  Google Scholar 

  • Wong RKS, Traub RD, Miles R (1984) Epileptogenic mechanisms as revealed by studies of the hippocampal slice. In: Schwartzkroin PA, HV Wheal (eds) Electrophysiology of epilepsy. Academic Press, London, pp 254–275.

    Google Scholar 

Download references

Authors
  1. D. A. Brown
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. J. Docherty
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B. H. Gahwiler
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. V. Halliwell
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Physiologisches Institut, Universität Freiburg, Hermann-Herder-Straße 7, D-7800, Freiburg i. Br., Germany

    Albrecht Fleckenstein

  2. Department of Pharmacology, University of Miami School of Medicine, P.O. Box 016189, Miami, FL, 33101, USA

    Cornelius Van Breemen D.V.M., Ph.D.

  3. Abteilung Herz-Kreislauf-Pharmakologie II, Bayer AG, Institut für Pharmakologie, Postfach 10 1709, D-5600, Wuppertal 1, Germany

    Rainer Gross

  4. Bayer AG, Institut für Pharmakologie, Postfach 10 1709, D-5600, Wuppertal 1, Germany

    Friedrich Hoffmeister

Rights and permissions

Reprints and permissions

Copyright information

© 1985 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Brown, D.A., Docherty, R.J., Gahwiler, B.H., Halliwell, J.V. (1985). Calcium Currents in Mammalian Central Neurones. In: Fleckenstein, A., Van Breemen, C., Gross, R., Hoffmeister, F. (eds) Cardiovascular Effects of Dihydropyridine-Type Calcium Antagonists and Agonists. Bayer-Symposium, vol 9. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-70499-4_5

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-70499-4_5

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-70501-4

  • Online ISBN: 978-3-642-70499-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation