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Detection of Antiviral IgM Antibodies and its Problems — A Review

  • Chapter
New Developments in Diagnostic Virology

Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 104))

Abstract

The presence of antibody activity in various serum immunoglobulin fractions was discovered as early as the 1930s (Heidelberger and Pedersen 1937; Kabat 1939), but it took another 20 years before the specific characteristics of antibodies having different molecular weights were recognized (Stelos 1958). Subsequent studies conducted in experimental animals and in man using various antigens, including viruses, indicated that the first immunoglobulins which appear after a primary antigenic stimulus are of the IgM class. These then disappear rapidly, usually within a few weeks, and are replaced by IgG antibodies that persist for a longer period. IgG is also the predominant antibody class in secondary immune responses (Bauer and Stavitsky 1961; Uhr and Finkelstein 1963; Svehag and Mandel 1964). The precedence of IgM antibodies observed in these early studies was later criticized as being a methodological error (Osler 1978), and in some more recent studies IgM and IgG (and IgA) antibodies have been shown to appear almost simultaneously but with individual variation (Cradock-Watson et al. 1979a; Halonen et al. 1979a).

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References

  • Aaskov JG, Davies CEA (1979) An immunofluorescence assay for human antibodies to Ross river virus. J Immunol Methods 25: 37–41

    Article  PubMed  CAS  Google Scholar 

  • Abe Y, Inouye S (1979) Complement-fixing immunoglobulin M antibody response in patients with infantile gastroenteritis. J Clin Microbiol 9: 284–287

    PubMed  CAS  Google Scholar 

  • Al-Nakib W (1980) A modified passive-haemagglutination technique for the detection of cytomegalo-virus and herpes simplex virus antibodies: application in virus-specific IgM diagnosis. J Med Virol 5: 287–293

    Article  PubMed  CAS  Google Scholar 

  • Ankerst J, Christensen P, Kjellén L, Kronvall G (1974) A routine diagnostic test for IgA and IgM antibodies to rubella virus: absorption of IgG with Staphylococcus aureus. J Infect Dis 130: 268–273

    Article  PubMed  CAS  Google Scholar 

  • Arstila P, Vuorimaa T, Kalimo K, Halonen P (1977) A so lid-phase radioimmunoassay for IgG and IgM antibodies against measles virus. J Gen Virol 34: 167–176

    Article  PubMed  CAS  Google Scholar 

  • Arvin AM, Koropchak CM (1980) Immunoglobulins M and G to varicella-zoster virus measured by solid-phase radioimmunoassay: antibody responses to varicella and herpes zoster infections. J Clin Microbiol 12: 367–374

    PubMed  CAS  Google Scholar 

  • Banatvala JE, Best JM, Kennedy EA, Smith EE, Spence ME (1967) A serological method for

    Google Scholar 

  • demonstrating recent infection by rubella virus. Br Med J 3:285–286

    Google Scholar 

  • Barros MF, Lebon P (1975) Séparation des anticorps IgM anti-rubéole par chromatographic d’affinité. Biomedicine [Express] 23: 184–188

    CAS  Google Scholar 

  • Baublis JV, Brown GC (1968) Specific response of the immunoglobulins to rubella infection. Proc Soc Exp Biol Med 128: 206–210

    PubMed  CAS  Google Scholar 

  • Bauer DC, Stavitsky AB (1961) On the different molecular forms of antibody synthesized by rabbits during the early response to a single injection of protein and cellular antigens. Proc Natl Acad Sci USA 47: 1676–1679

    Article  Google Scholar 

  • Best JM, Banatvala JE, Watson D (1969) Serum IgM and IgG responses in postnatally acquired rubella. Lancet 2: 65–68

    Article  PubMed  CAS  Google Scholar 

  • Bjørvatn B (1974) Incidence and persistence of mumps-specific IgM and IgA in the sera of mumps patients. Scand J Infect Dis 6: 125–129

    PubMed  Google Scholar 

  • Bolton AE, Hunter WM (1973) The labeling of proteins to high specific radioactivities by conjugation to a 125I-containing alkylating agent. Application to the radioimmunoassay. Biochem J 133: 529–538

    PubMed  CAS  Google Scholar 

  • BouéA, Nicolas A, Montagnon B (1971) Reinfection with rubella in pregnant women. Lancet 1: 1251–1253

    Google Scholar 

  • Bradley DW, Maynard JE, Hindman SH, Hornbeck CL, Fields HA, McCaustland KA, Cook EH Jr (1977) Serodiagnosis of viral hepatitis A: detection of acute-phase immunoglobulin M anti-hepatitis A virus by radioimmunoassay. J Clin Microbiol 5: 521–530

    PubMed  CAS  Google Scholar 

  • Bradley DW, Fields HA, McCaustland KA, Maynard JE, Decker RH, Whittington R, Overby LR (1979) Serodiagnosis of viral hepatitis A by a modified competitive binding radioimmuno-assay for immunoglobulin M anti-hepatitis A virus. J Clin Microbiol 9: 120–127

    PubMed  CAS  Google Scholar 

  • Braun R, Doerr HW, Horing C (1981) Rapid detection of rubella-specific IgM antibodies by the use of microimmunobeads (MIB-IgM). J Virol Methods 3: 45–49

    Article  PubMed  CAS  Google Scholar 

  • Brown GC, Baublis JV, O’Learly TP (1970) Development and duration of mumps fluorescent antibodies in various immunoglobulin fractions of human serum. J Immunol 104: 86–94

    PubMed  CAS  Google Scholar 

  • Brzosko WJ, Mikulska B, Cianciara J, Babiuch L (1975) Immunoglobulin classes of antibody to hepatitis B core antigen. J Infect Dis 132: 1–5

    Article  PubMed  CAS  Google Scholar 

  • Buchner YI, Heath RB, Collins JV, Pattison JR (1977) Serum IgM antibody and influenza A infection. J Clin Pathol 30: 723–727

    Article  PubMed  CAS  Google Scholar 

  • Biirgin-Wolff A, Hernandez R, Just M (1971) Separation of rubella IgM, IgA, and IgG antibodies of gel filtration on agarose. Lancet 2: 1278–1280

    Article  Google Scholar 

  • Burke DS, Nisalak A (1982) Detection of Japanese encephalitis virus immunoglobulin M anti-bodies in serum by antibody capture radioimmunoassay. J Clin Microbiol 15: 353–361

    PubMed  CAS  Google Scholar 

  • Capel PJA, Gerlag PGG, Hagemann JFH, Koene RAP (1980) The effect of 2-mercaptoethanol on IgM and IgG antibody activity. J Immunol Methods 36: 77–80

    Article  PubMed  CAS  Google Scholar 

  • Cappel R, de Cuyper F, de Braekeleer J (1978) Rapid detection of IgG and IgM antibodies for cytomegalovirus by the enzyme-linked immunosorbent assay (ELISA). Arch Virol 58:253– 258

    Article  PubMed  CAS  Google Scholar 

  • Catt K, Tregear GW (1967) Solid-phase radioimmunoassay in antibody-coated tubes. Science 158: 1570–1572

    Article  PubMed  CAS  Google Scholar 

  • Caul EO, Smyth GW, Clarke SKR (1974) A simplified method for the detection of rubella-specific IgM employing sucrose density fractionation and 2-mercaptoethanol. J Hyg (Camb) 73: 329–340

    CAS  Google Scholar 

  • Caul E0, Hobbs SJ, Roberts PC, Clarke SKR (1978) Evaluation of a simplified sucrose gradient method for the detection of rubella-specific IgM in routine diagnostic practice. J Med Virol 2: 153–163

    Article  PubMed  CAS  Google Scholar 

  • Chantier S, Devries E, Allen PR, Hum BAL (1976) A rapid immunofluorescent procedure for the detection of specific IgG and IgM antibody in sera using Staphylococcus aureus and latex- IgG as absorbents. J Immunol Methods 13: 367–380

    Article  Google Scholar 

  • Charlton D, Blandford G (1975) A solid-phase micro-radioimmunoassay to detect minute amounts of Ig class-specific anti-viral antibody in a mouse model system. J Immunol Methods 8:319– 330

    Google Scholar 

  • Cohen BJ (1978) The IgM antibody responses to the core antigen of hepatitis B virus. J Med Virol 3: 141–149

    Article  PubMed  CAS  Google Scholar 

  • Cohen IR, Norins LC, Julian AJ (1967) Competition between, and effectiveness of, IgG and IgM antibodies in indirect fluorescent antibody and other tests. J Immunol 98: 143–149

    PubMed  CAS  Google Scholar 

  • Cohen SM, Ducharme CP, Carpenter CA, Deibel R (1968) Rubella antibody in IgG and IgM immune-globulins detected by immunofluorescence. J Lab Clin Med 72: 760–766

    PubMed  CAS  Google Scholar 

  • Cossio PM, Rabinovich A, Maiztegui JI, Carballal G, Casanova MB, Ritacco V, Arana RM (1979) Immunofluorescent anti-Junin virus antibodies in Argentine hemorrhagic fever. Intervirology 12: 26–31

    Article  PubMed  CAS  Google Scholar 

  • Cradock-Watson JE, Bourne MS, Vandervelde EM (1972) IgG, IgA and IgM responses in acute rubella determined by the immunofluorescent technique. J Hyg (Camb) 70: 473–485

    Article  CAS  Google Scholar 

  • Cradock-Watson JE, Ridehalgh MKS, Chantier S (1976) Specific immunoglobulins in infants with the congenital rubella syndrome. J Hyg (Camb) 76: 109–123

    Article  CAS  Google Scholar 

  • Cradock-Watson JE, Ridehalgh MKS, Bourne MS (1979a) Specific immunoglobulin responses after varicella and herpes zoster. J Hyg (Camb) 28: 319–336

    Article  Google Scholar 

  • Cradock-Watson JE, Ridehalgh MKS, Pattison JR, Anderson MJ, Kangro HO (1979b) Comparison of immunofluorescence and radioimmunoassay for detecting IgM antibody in infants with the congenital rubella syndrome. J Hyg (Camb) 83: 413–423

    Article  CAS  Google Scholar 

  • Cranage MP, Gardner PS (1980) Systemic cell-mediated and antibody responses in infants with respiratory syncytial virus infections. J Med Virol 5:161–170

    Article  Google Scholar 

  • Dali V, Jensen NH, Hansen U (1980) Diagnosis of pre- and postnatal rubella by demonstration of specific IgM-class antibodies by a microplate immunofluorescence test. Med Microbiol Immunol 168: 73–80

    Article  Google Scholar 

  • Daugharty H, Warfield DT, Hemingway WD, Casey HL (1973) Mumps class-specific immune-globulins in radioimmunoassay and conventional serology. Infect Immun 7: 380–385

    PubMed  CAS  Google Scholar 

  • Decker RH, Kosakowski SM, Vanderbilt AS, Ling C-M, Chairez R, Overby LR (1981) Diagnosis of acute hepatitis A by HAVAB-M, a direct radioimmunoassay for IgM anti-HAV. Am J Clin Pathol 76: 140–147

    PubMed  CAS  Google Scholar 

  • Denoyel GA, Gaspar A, Peyramond D (1981) Diagnosis of recent rubella virus infection by demonstration of specific immunoglobulin M antibodies: comparison of solid-phase reverse immunosorbent test with sucrose density gradient centrifugation. J Clin Microbiol 13: 698–704

    PubMed  CAS  Google Scholar 

  • Desmyter J, South MA, Rawls WE (1971) The IgM antibody response in rubella during pregnancy. J Med Microbiol 4: 107–114

    Article  CAS  Google Scholar 

  • Dibbert H-J (1976) Antikörper der Immunoglobuline 19S and 7S bei der Röteln-Diagnostik Zentralbl Bakteriol [Orig A] 234: 145–158

    CAS  Google Scholar 

  • Diment JA, Chantier SM (1981) Enzyme immunoassay for detection of rubella-specific IgM anti-body. Lancet 1: 394–395

    Article  PubMed  CAS  Google Scholar 

  • Dittmar D, Cleary TJ, Castro A (1979) Immunoglobulin G- and M-specific enzyme-linked immune-sorbent assay for detection of dengue antibodies. J Clin Microbiol 9: 498–502

    PubMed  CAS  Google Scholar 

  • Doerr HW (1979) Rasche Isolierung der Serum-IgM-Antikörper für den Röteln-Hämagglutionations-hemmungstest. Aerztl Lab 25: 178–179

    Google Scholar 

  • Doerr HW, Fretschner R, Geisen HP (1980) Virusspezifischer IgM-Nachweis mit Routinemethoden. Zentralbl Bakteriol [Orig A] 246: 158–166

    CAS  Google Scholar 

  • Dörries R, ter Meulen V (1980) Detection of enterovirus-specific IgG and IgM antibodies in humans by an indirect solid-phase radioimmunoassay. Med Microbiol Immunol 168: 159–171

    Article  PubMed  Google Scholar 

  • Dormeyer HH, Arnold W, Kryger P, Nielsen JO, Meyer zum Büschenfelde KH (1981) IgM anti-body to hepatitis B core antigen (anti-HBc IgM) in “healthy” HBgAG carriers: a longitudinal study of 75 cases. Klin Wochenschr 59: 675–678

    Article  Google Scholar 

  • Duermeyer W, van der Veen J (1978) Specific detection of IgM antibodies by ELISA, applied in hepatitis-A. Lancet 2: 684–685

    Article  PubMed  CAS  Google Scholar 

  • Duermeyer W, Wielaard F, van der Veen J (1979) A new principle for the detection of specific IgM antibodies applied in an ELISA for hepatitis A. J Med Virol 4: 25–32

    Article  PubMed  CAS  Google Scholar 

  • Edelman R, Pariyanonda A (1973) Human immunoglobulin M antibody in the serodiagnosis of Japanese encephalitis virus infections. Am J Epidemiol 98: 29–38

    PubMed  CAS  Google Scholar 

  • Edelman R, Schneider RJ, Vejjajiva A, Pornpibul R, Voodhikul P (1976) Persistence of virus- specific IgM and clinical recovery after Japanese encephalitis. Am J Trop Med Hyg 25:733– 738

    Google Scholar 

  • Field PR, Murphy AM (1972) The role of specific IgM globulin estimations in the diagnosis of acquired rubella. Med J Aust 2: 1244–1248

    PubMed  CAS  Google Scholar 

  • Field PR, Shanker S, Murphy AM (1980) The use of protein A sepharose affinity chromatography for separation and detection of specific IgM antibody in acquired rubella infection: a comparison with absorption by staphylococci containing protein A and density gradient ultracentrifu- gation. J Immunol Methods 32: 59–70

    Article  PubMed  CAS  Google Scholar 

  • Flehmig B (1978) Laboratoriumsdiagnose der Hepatitis A-Infektion. Bundesgesundheitsblatt 21: 277–283

    Google Scholar 

  • Flehmig B, Ranke M, Berthold H, Gerth H-J (1979) A solid-phase radioimmunoassay for detection of IgM antibodies to hepatitis A virus. J Infect Dis 140: 169–175

    Article  PubMed  CAS  Google Scholar 

  • Flower AJE, Banatvala JE, Chrystie IL (1977) BK antibody and virus-specific IgM responses in renal transplant recipients, patients with malignant disease, and healthy people. Br Med J 2: 220–223

    Article  PubMed  CAS  Google Scholar 

  • Forghani B, Schmidt NJ (1979) Antigen requirements, sensitivity, and specificity of enzyme immunoassays for measles and rubella viral antibodies. J Clin Microbiol 9:657–664

    PubMed  CAS  Google Scholar 

  • Forghani B, Schmidt NJ, Lennette EH (1973) Demonstration of rubella IgM antibody by indirect fluorescent antibody staining, sucrose density gradient centrifugation and mercapto- ethanol reduction. Intervirology 1: 48–59

    Article  PubMed  CAS  Google Scholar 

  • Forsgren A, Sjoquist J (1966) “Protein A” from S. aureus. I. Pseudo-immune reaction with human globulin. J Immunol 97:822

    Google Scholar 

  • Frisch-Niggemeyer W (1975) Rapid separation of immunoglobulin M from immunoglobulin G antibodies for reliable diagnosis of recent rubella infections. J Clin Microbiol 2: 377–381

    PubMed  CAS  Google Scholar 

  • Frisch-Niggemeyer W (1982) A solid-phase radioimmunoassay for quantitative measurement of class-specific antibodies against tick-borne encephalitis virus. J Virol Methods 3: 319–328

    Article  PubMed  CAS  Google Scholar 

  • Frosner GG, Scheid R, Wolf H, Deinhardt F (1979) Immunoglobulin M anti-hepatitis A virus determination by reorienting gradient centrifugation for diagnosis of acute hepatitis A. J Clin Microbiol 9: 476–478

    PubMed  CAS  Google Scholar 

  • Geisen HP, Frank R, Doerr HW, Enders G (1979) Simple method to detect virus-specific IgM antibodies in patients’ serum samples after immunosorption of immunoglobulins G and A. Med Microbiol Immunol 167: 77–82

    Article  PubMed  CAS  Google Scholar 

  • Gerlich WH, Liier W (1979) Selective detection of IgM antibody against core antigen of the hepatitis B virus by a modified enzyme immune assay. J Med Virol 4: 227–238

    Article  PubMed  CAS  Google Scholar 

  • Gerlich WH, Liier W, Thomssen R (The Study Group for Viral Hepatitis of the Deutsche For- schungsgemeinschaft) (1980) Diagnosis of acute and inapparent hepatitis B virus infections by measurement of IgM antibody to hepatitis B core antigen. J Infect Dis 142: 95–101

    Article  PubMed  CAS  Google Scholar 

  • GernaG, Chambers RW (1977) Rapid detection of human cytomegalovirus and herpesvirus hominis IgM antibody by the immunoperoxidase technique. Intervirology 8: 257–271

    Article  Google Scholar 

  • Gibson PE, Field AM, Gardner SD, Coleman DV (1981) Occurrence of IgM antibodies against BK and JC polyomaviruses during pregnancy. J Clin Pathol 34: 674–679

    Article  PubMed  CAS  Google Scholar 

  • Gispen R, Nagel J, Brand-Saathof B, Degraaf S (1975) Immunofluorescence test for IgM rubella antibodies in whole serum after absorption with antkyFc. Clin Exp Immunol 22: 431–437

    PubMed  CAS  Google Scholar 

  • Goldwater PN, Banatvala JE (1981) Solid-phase immunosorbent haemadsorption (SPIHAd): a new, rapid and simple test for rubella specific Igm. J Virol Methods 2: 341–347

    Article  PubMed  CAS  Google Scholar 

  • Goldwater PN, Webster M, Banatvala JE (1982) Use of a simple, new test for virus-specific IgM to investigate an outbreak of influenza B in a hospitalized aged community. J Virol Methods 4: 9–18

    Article  PubMed  CAS  Google Scholar 

  • Gravell M, Dorsett PH, Gutenson O, Ley AC (1977) Detection of antibody to rubella virus by enzyme-linked immunosorbent assay. J Infect Dis 136: S300–303

    Article  PubMed  Google Scholar 

  • Gupta JD, Peterson V, Stout M, Murphy AM (1971) Single-sample diagnosis of recent rubella by fractionation of antibody on Sephadex G-200 column. J Clin Pathol 24: 547–550

    Article  PubMed  CAS  Google Scholar 

  • Hacham M, Leventon-Kriss S, Sarov I (1980) Enzyme-linked immunosorbent assay for detection of virus-specific IgM antibodies to varicella-zoster virus. Intervirology 13: 214–222

    Article  PubMed  CAS  Google Scholar 

  • Haire M, Madden DSM (1970) Immunoglobulin responses in rubella and its complications. Br Med J 3: 130–132

    Article  PubMed  CAS  Google Scholar 

  • Haire M, Adair BMcC, Fraser KB (1972) The effect of temperature and time of incubation in the preparation of antigens for detection of virus-specific IgM antibody. Clin Exp Immunol 13: 327–333

    Google Scholar 

  • Halonen P, Bennich H, Torfason E, Karlsson T, Ziola B, Matikainen M-T, Hjertsson E, Wesslen T (1979a) Solid-phase radioimmunoassay of serum immunoglobulin A antibodies to respiratory syncytial virus and adenovirus. J Clin Microbiol 10: 192–197

    PubMed  CAS  Google Scholar 

  • Halonen P, Meurman O, Matikainen M-T, Torfason E, Bennich H (1979b) IgA antibody response in acute rubella determined by solid-phase radioimmunoassay. J Hyg (Lond) 83: 69–75

    Article  CAS  Google Scholar 

  • Handsher R, Fogel A (1977) Modified staphylococcal absorption method used for detecting rubella-specific immunoglobulin M antibodies during a rubella epidemic. J Clin Microbiol 5: 588–592

    PubMed  CAS  Google Scholar 

  • Hanshaw JB, Steinfeld HJ, White CJ (1968) Fluorescent-antibody test for cytomegalovirus macro-globulin. N Engl J Med 279: 566–570

    Article  PubMed  CAS  Google Scholar 

  • Hanshaw JB, Niederman JC, Chessin LN (1972) Cytomegalovirus macroglobulin in cell-associated herpesvirus infections. J Infect Dis 125: 304–306

    Article  PubMed  CAS  Google Scholar 

  • Harcourt GC, Best JM, Banatvala JE (1980) Rubella-specific serum and nasopharyngeal antibodies in volunteers with naturally acquired and vaccine-induced immunity after intranasal challenge. J Infect Dis 142: 145–155

    Article  PubMed  CAS  Google Scholar 

  • Hawkes RA, Boughton CR, Ferguson V, Lehmann NI (1980) Use of immunoglobulin M antibody to hepatitis B core antigen in diagnosis of viral hepatitis. J Clin Microbiol 11: 581–583

    PubMed  CAS  Google Scholar 

  • Hayashi K, Rosenthal J, Notkins AL (1972) Iodine 125-labeled antibody to viral antigens: binding to the surface of virus-infected cells. Science 176: 516–518

    Article  PubMed  CAS  Google Scholar 

  • Heidelberger M, Pedersen KO (1937) The molecular weight of antibodies. J Exp Med 65: 393–414

    Article  PubMed  CAS  Google Scholar 

  • Heinz FX, Roggendorf M, Hofmann H, Kunz C, Deinhardt F (1981) Comparison of two different enzyme immunoassays for detection of immunoglobulin M antibodies against tick-borne encephalitis virus in serum and cerebrospinal fluid. J Clin Microbiol 14: 141–146

    PubMed  CAS  Google Scholar 

  • Hekker AC, Brand-Saathof B, Vis J, Meijers RC (1979) Indirect immunofluorescence test for detection of IgM antibodies to cytomegalovirus. J Infect Dis 140: 596–600

    Article  PubMed  CAS  Google Scholar 

  • Hofmann H, Frisch-Niggemeyer W, Heinz F (1979) Rapid diagnosis of tick-borne encephalitis by means of enzyme-linked immunosorbent assay. J Gen Virol 42: 505–511

    Article  PubMed  CAS  Google Scholar 

  • Hornsleth A, Leerhøy J, Grauballe P, Spanggaard H (1974) Rubella-specific IgM and IgA antibodies. The indirect immunofluorescence (IF)-technique applied to sera with reduced IgG concentration. Acta Pathol Microbiol Scand [B] 82: 742–747

    Google Scholar 

  • Hornsleth A, Leerhoy J, Grauballe P, Spanggaard H (1975) Persistence of rubella virus-specific immunoglobulin M and immunoglobulin A antibodies: investigation of successive serum samples with lowered immunoglobulin G concentration. Infect Immun 11: 804–808

    PubMed  CAS  Google Scholar 

  • Hunter WM, Greenwood FC (1962) Preparation of iodine 131-labeled human growth hormone of high specific activity. Nature 194:495–496

    Article  Google Scholar 

  • Hutchinson HD, Ziegler DW (1972) Simplified radioimmunoassay for diagnostic serology. Appl Microbiol 24: 742–749

    PubMed  CAS  Google Scholar 

  • Inouye S, Kono R (1981) Simple technique for separation of concentrated immunoglobulin M fractions by multi-sample gel chromatography and its application to rubella serodiagnosis. J Clin Microbiol 13: 248–251

    PubMed  CAS  Google Scholar 

  • Ishii K, Matsunaga Y, Kono R (1968) Immunoglobulins produced in response to Japanese encephalitis virus infections of man. J Immunol 101: 770–775

    PubMed  CAS  Google Scholar 

  • Jansen P (1975) Diagnostik von intrauterinen Infektionen mit Zytomegalie und Rotelnvirus. Med Klin 70: 267–269

    PubMed  CAS  Google Scholar 

  • Johnson RB Jr, Libby R (1980) Separation of immunoglobulin M (IgM) essentially free of IgG from serum for use in systems requiring assay of IgM-type antibodies without interference from rheumatoid factor. J Clin Microbiol 12: 451–454

    PubMed  CAS  Google Scholar 

  • Jung M, Krech U, Price PC, Pyndiah MN (1975) Evidence of chronic persistent infections with polyomaviruses (BK type) in renal transplant recipients. Arch Virol 47: 39–46

    Article  PubMed  CAS  Google Scholar 

  • Kabat EA (1939) The molecular weight of antibodies. J Exp Med 69: 103–118

    Article  PubMed  CAS  Google Scholar 

  • Kalimo KOK, Marttila RJ, Granfors K, Viljanen MK (1978) Solid-phase radioimmunoassay of human immunoglobulin M and immunoglobulin G antibodies against herpes simplex virus type 1 capsid, envelope, and excreted antigens. Infect Immun 15: 883–889

    Google Scholar 

  • Kangro HO (1980) Evaluation of a radioimmunoassay for IgM-class antibodies against cytomegalovirus. Br J Exp Pathol 61: 512–520

    PubMed  CAS  Google Scholar 

  • Kangro HO, Pattison JR, Heath RB (1978) The detection of rubella-specific IgM antibodies by radioimmunoassay. Br J Exp Pathol 59: 577–583

    PubMed  CAS  Google Scholar 

  • Kangro HO, Jackson C, Heath RB (1981) Comparison of radioimmunoassay and the gel filtration technique for routine diagnosis of rubella during pregnancy. J Hyg (Lond) 87: 249–255

    Article  CAS  Google Scholar 

  • Katze MG, Crowell RL (1980) Immunological studies of the group B coxsackieviruses by the sandwich enzyme-linked immunosorbent assay (ELISA) and immunoprecipitation. J Gen Virol 50: 357–367

    Article  PubMed  CAS  Google Scholar 

  • Knez V, Stewart JA, Ziegler DW (1976) Cytomegalovirus-specific IgM and IgG response in humans studied by radioimmunoassay. J Immunol 117: 2006–2013

    PubMed  CAS  Google Scholar 

  • Krech U, Wilhelm JA (1979) A solid-phase immunosorbent technique for the rapid detection of rubella IgM by hemagglutination inhibition. J Gen Virol 44: 281–286

    Article  PubMed  CAS  Google Scholar 

  • Krishna RV, Meurman OH, Ziegler T, Krech UH (1980) Solid-phase enzyme immunoassay for determination of antibodies to cytomegalovirus. J Clin Microbiol 12: 46–51

    PubMed  CAS  Google Scholar 

  • Kronvall G, Williams RC Jr (1969) Differences in anti-protein A activity among IgG subgroups. J Immunol 103: 828–833

    PubMed  CAS  Google Scholar 

  • Kryger P, Mathiesen LR, Miller AM, Aldershvile J, Hansson BG, Nielsen JO (1981) Enzyme-linked immunosorbent assay for detection of immunoglobulin M antibody to hepatitis B core antigen. J Clin Microbiol 13: 405–409

    PubMed  CAS  Google Scholar 

  • Kunz C, Hofmann H (1971) Die Frühdiagnose der Frühsommer-Meningoenzephalitis (FSME) im Hämagglutinationshemmungstest durch Behandlung des Serums mit 2-Mercaptoäthanol. Zentralb 1 Bakteriol [Orig A] 218: 273–279

    CAS  Google Scholar 

  • Kurtz JB (1974) Specific IgG and IgM antibody responses in herpes simplex-virus infections. J Med Microbiol 7: 333–341

    Article  PubMed  CAS  Google Scholar 

  • Lander JJ, Holland PV, Alter HJ, Chanock RM, Purcell RH (1972) Antibody to hepatitis-associated antigen. Frequency and pattern of response as detected by radioimmunoprecipitation. JAMA 220: 1079–1082

    Article  PubMed  CAS  Google Scholar 

  • Lehrich JR, Kassel JA, Rossen RD (1966) Immunoglobulin classes of neutralizing antibody formed after human inoculation with soluble adenoviral antigens. J Immunol 97: 654–662

    PubMed  CAS  Google Scholar 

  • Leidel J, Mertens TH, Eggers HJ (1977) Auftreten und Persistenz rötelnspezifischer IgM-Antikör-per. Dtsch Med Wochenschr 102: 1418–1421

    Article  PubMed  CAS  Google Scholar 

  • Leinikki PO, Shekarchi I, Dorsett P, Sever JL (1978) Determination of virus-specific IgM antibodies by using ELISA: elimination of false-positive results with protein A-Sepharose absorption and subsequent IgM antibody assay. J Lab Clin Med 92: 849–857

    PubMed  CAS  Google Scholar 

  • Lemon SM, Gates NL, Simms TE, Bancroft WH (1981) IgM antibody to hepatitis B core antigen as a diagnostic parameter of acute infection with hepatitis B virus. J Infect Dis 143:803– 809

    Google Scholar 

  • Locarnini SA, Ferris AA, Lehmann NI, Gust ID (1977) The antibody response following hepatitis A infection. Intervirology 8: 309–318

    Article  PubMed  CAS  Google Scholar 

  • Luton P, Ridgway GL (1979) Use of the ultracentrifuge vertical rotor in the detection of rubella-specific IgM on a sucrose density gradient. J Clin Pathol 32: 931–934

    Article  PubMed  CAS  Google Scholar 

  • McLean B, Sonza S, Holmes IH (1980) Measurement of immunoglobulin A, G, and M class rotavirus antibodies in serum and mucosal secretions. J Clin Microbiol 12: 314–319

    PubMed  CAS  Google Scholar 

  • Meurman OH (1978) Persistence of immunoglobulin G and immunoglobulin M antibodies after postnatal rubella infection determined by solid-phase radioimmunoassay. J Clin Microbiol 7: 34–38

    PubMed  CAS  Google Scholar 

  • Meurman OH, Ziola BR (1978) IgM-class rheumatoid factor interference in the so lid-phase radio-immunoassay of rubella-specific IgM antibodies. J Clin Pathol 31: 483–487

    Article  PubMed  CAS  Google Scholar 

  • Meurman OH, Viljanen MK, Granfors K (1977) Solid-phase radioimmunoassay of rubella virus immunoglobulin M antibodies: comparison with sucrose density gradient centrifugation test. J Clin Microbiol 5: 257–262

    PubMed  CAS  Google Scholar 

  • Meurman O, Terho P, Salmi A (1978) Activation of rheumatoid factor during pregnancy. Lancet 2: 685–686

    Article  PubMed  CAS  Google Scholar 

  • Meurman OH, Matter L, Krishna RV, Krech UH (1981) A “reverse” solid-phase radioimmunoassay for IgM-antibodies to hepatitis A virus. Acta Pathol Microbiol Scand [C] 89: 79–84

    CAS  Google Scholar 

  • Meurman O, Hanninen P, Krishna RV, Ziegler T (to be published) Determination of IgG- and IgM-class antibodies to mumps virus by solid-phase enzyme immunoassay. J Virol Methods 4

    Google Scholar 

  • Minor TE, Helstrom PB, Nelson DB, D’Alessio DJ (1979) Counterimmunoelectrophoresis test for immunoglobulin M antibodies to group B coxsackievirus. J Clin Microbiol 9: 503–506

    PubMed  CAS  Google Scholar 

  • Miller AM, Mathiesen LR (1979) Detection of immunoglobulin M antibodies to hepatitis A virus by enzyme-linked immunosorbent assay. J Clin Microbiol 10: 628–632

    Google Scholar 

  • Monath TPC (1971) Neutralizing antibody responses in the major immunoglobulin classes to yellow fever 17D vaccination of humans. Am J Epidermiol 93: 122–129

    CAS  Google Scholar 

  • Morgan-Capner P, Davies E, Pattison JR (1980) Rubella-specific IgM detection using Sephacryl S-300 gel filtration. J Clin Pathol 33: 1082–1085

    Article  PubMed  CAS  Google Scholar 

  • Mortimer PP, Tedder RS, Hambling MH, Shafi MS, Burkhardt F, Schilt U (1981a) Antibody capture radioimmunoassay for anti-rubella IgM. J Hyg (Lond) 86: 139–153

    Article  CAS  Google Scholar 

  • Mortimer PP, Vandervelde EM, Parry JV, Cohen BJ, Tedder RS (1981b) The anti-HBc IgM response in the acute and convalescent phases of acute hepatitis. J Infect 3: 339–347

    Article  PubMed  CAS  Google Scholar 

  • Nagington J (1971) Cytomegalovirus antibody production in renal transplant patients. J Hyg (Camb) 69: 645–660

    Article  CAS  Google Scholar 

  • Nagy G, Mezey I (1977) The use of ion exchange chromatography for demonstration of rubella-specific IgM antibodies. Acta Microbiol Acad Sci Hung 24: 189–194

    PubMed  CAS  Google Scholar 

  • Naot Y, Barnett EV, Remington JS (1981) Method for avoiding false positive results occurring in immunoglobulin M enzyme-linked immunosorbent assays due to presence of both rheumatoid factor and antinuclear antibodies. J Clin Microbiol 14: 73–78

    PubMed  CAS  Google Scholar 

  • Nicolai-Scholten ME, Ziegelmaier R, Behrens F, Hopken W (1980) The enzyme-linked immunosorbent assay (ELISA) for determination of IgG and IgM antibodies after infection with mumps virus. Med Microbiol Immunol 168: 81–90

    Article  PubMed  CAS  Google Scholar 

  • Nikoskelainen J, Hanninen P (1975) Antibody response to Epstein-Barr virus in infectious mononucleosis. Infect Immun 11: 42–51

    PubMed  CAS  Google Scholar 

  • Ogra PL, Karzon DT, Righthand F, MacGillivray M (1968) Immunoglobulin response in serum and secretions after immunization with live and inactivated poliovaccine and natural infection. N Engl J Med 279: 893–900

    Article  PubMed  CAS  Google Scholar 

  • Ogra PL, Kerr-Grant D, Umana G, Dzierba J, Weintraub D (1971) Antibody response in serum and nasopharynx after naturally acquired and vaccine-induced infection with rubella virus. N Engl J Med 285: 1333–1339

    Article  PubMed  CAS  Google Scholar 

  • Okuno T, Kondelis N (1978) Evaluation of dithiothreitol (DTT) for inactivation of IgM antibodies. J Clin Pathol 31: 1152–1155

    Article  PubMed  CAS  Google Scholar 

  • Ørstavik I, Haug KW (1976) Virus-specific IgM antibodies in acute gastroenteritis due to a reovirus-like agent (rotavirus). Scand J Infect Dis 8: 237–240

    PubMed  Google Scholar 

  • Osier AG (1978) On the precedence of 19S antibodies in the early immune response. Immuno- chemistry 15: 717–720

    Article  Google Scholar 

  • Pattison JR, Dane DS, Mace JE (1975) Persistence of specific IgM after natural infection with rubella virus. Lancet 1: 185–187

    Article  PubMed  CAS  Google Scholar 

  • Pattison JR, Jackson C (1978) The use of high speed gel filtration for the detection of rubella-specific IgM. J Immunol Methods 19: 269–277

    Article  PubMed  CAS  Google Scholar 

  • Pattison JR, Mace JE (1973) A simple, inexpensive gel filtration technique for use in diagnostic serology. J Clin Pathol 26: 309–311

    Article  PubMed  CAS  Google Scholar 

  • Pattison JR, Mace JE (1975a) The detection of specific IgM antibodies following infection with rubella virus. J Clin Pathol 28: 377–382

    Article  PubMed  CAS  Google Scholar 

  • Pattison JR, Mace JE (1975b) Elution patterns of rubella IgM, IgA, and IgG antibodies from a dextran and an agarose gel. J Clin Pathol 28: 670–673

    Article  PubMed  CAS  Google Scholar 

  • Pattison JR, Mace JE, Dane DS (1976) The detection and avoidance of false-positive reactions in tests for rubella-specific IgM. J Med Microbiol 9: 355–357

    Article  PubMed  CAS  Google Scholar 

  • Pattison JR, Jackson CM, Hiscock JA, Cradock-Watson JE, Ridehalgh MKS (1978) Comparison of methods for detecting specific IgM antibody in infants with congenital rubella. J Med Microbiol 11: 411–418

    Article  PubMed  CAS  Google Scholar 

  • Pead PJ (1974) Estimation of rubella-specific IgM antibody in sera by single fraction collection on Sephadex G-200. Med Lab Technol 31: 159–162

    PubMed  CAS  Google Scholar 

  • Popow-Kraupp T (1981) Enzyme-linked immunosorbent assay (ELISA) for mumps virus anti-bodies. J Med Virol 8: 79–88

    PubMed  CAS  Google Scholar 

  • Price PC (1977) Direct radioimmunoassay for the detection in human serum of IgM-class antibodies against Mycoplasma pneumoniae. PhD thesis, University of Bern

    Google Scholar 

  • Pyndiah N, Price P, Jung M, Wilhelm J, Krech U (1977) A rapid chromatographic separation of IgM from 50 jul of whole serum: its application to diagnostic tests. Experientia 33:1678– 1679

    Google Scholar 

  • Reimer CB, Black CM, Phillips DJ, Logan LC, Hunter EF, Pender BJ, McGrew BE (1975) The specificity of fetal IgM: antibody or anti-antibody? Ann NY Acad Sci 254: 77–93

    Article  PubMed  CAS  Google Scholar 

  • Reiner M, Wecker E (1981) A modified absorption-reduction method to detect virus-specific hemagglutination inhibiting and neutralizing IgM antibodies. Med Microbiol Immunol 169: 237–245

    Article  PubMed  CAS  Google Scholar 

  • Robertson PW, Bell SM (1974) Prolonged rubella IgM antibody response. Med J Austr 2: 857

    CAS  Google Scholar 

  • Robertson PW, Kertesz V, Cloonan MJ (1977) Elimination of false positive cytomegalovirus immunoglobulin M-fluorescent-antibody reactions with immunoglobulin M serum fractions. J Clin Microbiol 6: 174–175

    PubMed  CAS  Google Scholar 

  • Roggendorf M, Schneweis KE, Wolff MH (1976) Zum Nachweis Röteln-spezifischer IgM im Hämag- glutinationshemmungstest. Vergleichende Untersuchungen mit der Absorption von IgG durch Protein-A-haltige Staphylokokken und der Dichtegradienten-Ultrazentrifugation. Zentralbl Bakteriol [Orig A] 235: 363–372

    Google Scholar 

  • Roggendorf M, Frösner GG, Deinhardt F, Scheid R (1980) Comparison of solid-phase test systems for demonstrating antibodies against hepatitis A virus (anti-Hav) of the IgM-class. J Med Virol 5: 47–62

    Article  PubMed  CAS  Google Scholar 

  • Roggendorf M, Deinhardt F, Frösner GG, Scheid R, Bayerl B, Zachoval R (1981a) Immunoglobulin M antibodies to hepatitis B core antigen: evaluation of enzyme immunoassay for diagnosis of hepatitis B virus infection. J Clin Microbiol 13: 618–626

    PubMed  CAS  Google Scholar 

  • Roggendorf M, Heinz F, Deinhardt F, Kunz C (1981b) Serological diagnosis of acute tick-borne encephalitis by demonstration of antibodies of the IgM class. J Med Virol 7: 41–50

    Article  PubMed  CAS  Google Scholar 

  • Roggendorf M, Wigand R, Deinhardt F, Frösner GG (1982) Enzyme-linked immunosorbent assay for acute adenovirus infection. J Virol Methods 4: 27–35

    Article  PubMed  CAS  Google Scholar 

  • Salonen E-M, Vaheri A, Suni J, Wager 0 (1980) Rheumatoid factor in acute viral infections: interference with determination of IgM, IgG, and IgA antibodies in an enzyme immunoassay. J Infect Dis 142: 250–255

    CAS  Google Scholar 

  • Sarkkinen HK, Meurman OH, Halonen PE (1979) Solid-phase radioimmunoassay of IgA, IgG, and IgA antibodies to human rotavirus. J Med Virol 3: 281–289

    Article  PubMed  CAS  Google Scholar 

  • Schluederberg A (1965) Immune globulins in human viral infections. Nature 205:1232–1233 Schmidt NJ, Lennette EH (1975) Neutralizing antibody responses to varicella-zoster virus. Infect Immun 12: 606–613

    Google Scholar 

  • Schmidt NJ, Lennette EH, Dennis J (1968) Characterization of antibodies produced in natural and experimental coxsackievirus infections. J Immunol 100: 99–106

    PubMed  CAS  Google Scholar 

  • Schmidt NJ, Magoffin RL, Lennette EH (1973) Association of group B coxsackieviruses with cases of pericarditis, myocarditis, or pleurodynia by demonstration of immunoglobulin M antibody. Infect Immun 8: 341–348

    PubMed  CAS  Google Scholar 

  • Schmidt WAK, Klein M (1980) A new method for the determination of virus-specific IgG and IgM antibodies. Arch Virol 66:67–70

    Google Scholar 

  • Schmitz H, Haas R (1972) Determination of different cytomegalovirus immunoglobulins (IgG, IgA, IgM) by immunofluorescence. Arch Gesamte Virusforsch 37: 131–140

    Article  PubMed  CAS  Google Scholar 

  • Schmitz H, Shimizu H, Kampa D, Doerr HW (1975) Rapid method to detect rubella immuno-globulin M and immunoglobulin A antibodies. J Clin Microbiol 1: 132–135

    PubMed  CAS  Google Scholar 

  • Schmitz H, Doerr HW, Kampa D, Vogt A (1977a) Solid-phase enzyme immunoassay for immunoglobulin M antibodies to cytomegalovirus. J Clin Microbiol 5: 629–634

    PubMed  CAS  Google Scholar 

  • Schmitz H, Kampa D, Doerr HW, Luthardt T, Hillemanns HG, Wurtele A (1977b) IgM antibodies to cytomegalovirus during pregnancy. Arch Virol 53: 177–184

    Article  PubMed  Google Scholar 

  • Schmitz H, von Deimling U, Flehmig B (1980) Detection of IgM antibodies to cytomegalovirus (CMV) using an enzyme-labelled antigen (ELA). J Gen Virol 50: 59–68

    Article  PubMed  CAS  Google Scholar 

  • Scott RMcN, McCown JM, Russell PK (1972) Human immunoglobulin specificity after group B arbovirus infections. Infect Immun 6: 277–281

    PubMed  CAS  Google Scholar 

  • Sheinbergas MM, Verikene VV, Maslinskas VYu, Lyubetsky VB (1978) Specific immunofluorescent IgG, IgM, and IgA antibodies in lymphocytic choriomeningitis. Acta Virol (Praha) 22: 225–230

    Google Scholar 

  • Shirodaria PV, Fraser KB, Stanford F (1973) Secondary fluorescent staining of virus antigens by rheumatoid factor and fluorescein-conjugated anti-IgM. Ann Rheum Dis 32: 53–57

    Article  PubMed  CAS  Google Scholar 

  • Skaug K, Gaarder PI (1978) An indirect immunofluorescent antibody test for determination of rubella virus-specific IgM antibodies. Acta Pathol Microbiol Scand [C] 86: 33–35

    CAS  Google Scholar 

  • Skaug K, Tjøtta E (1974) Diagnosis of recent herpes simplex infections. A modified immunofluorescent test for the detection of specific herpes simplex IgM antibodies after staphylococcal absorption of IgG. Acta Pathol Microbiol Scand [B] 82: 323–328

    CAS  Google Scholar 

  • Smith CB, Bellanti JA, Chanock RM (1967) Immunoglobulins in serum and nasal secretions following infection with type 1 parainfluenza virus and injection of inactivated vaccines. J Immunol 99: 133–141

    PubMed  CAS  Google Scholar 

  • Stallman ND, Allan BC, Sutherland CJ (1974) Prolonged rubella IgM antibody response. Med J Aust 2: 629–631

    PubMed  CAS  Google Scholar 

  • Stelos P (1958) Comparative studies of rabbit hemolysins to various antigens. I. Hemolysins to beef red cells. J Infect Dis 102: 103–113

    Article  PubMed  CAS  Google Scholar 

  • Sugg U, Schneider W, Frosner GG, Schmidt K (1979) On the problems of efficacy of separation of IgM and IgG by different methods. Vox Sang 36: 25–28

    Article  PubMed  CAS  Google Scholar 

  • Sundqvist V-A, Wahren B (1981) An interchangeable ELISA for cytomegalovirus antigen and antibody. J Virol Methods 2: 301–312

    Article  PubMed  CAS  Google Scholar 

  • Svehag S-E, Mandel B (1964) The formation and properties of poliovirus neutralizing antibody. J Exp Med 119: 1–19, 21–39

    CAS  Google Scholar 

  • Swenson PD, Escobar MR, Galen EA, Carithers RL (1981) Determination of immunoglobulin M antibodies against hepatitis B core antigen and hepatitis A virus by reorienting sucrose gradient high-speed centrifugation of diagnosis of acute viral hepatitis. J Clin Microbiol 14: 544–549

    PubMed  CAS  Google Scholar 

  • Tedder RS, Wilson-Croome R (1980) Detection by radioimmunoassay of IgM-class antibody to hepatitis B core antigen: a comparison of two methods. J Med Virol 6: 235–247

    Article  PubMed  CAS  Google Scholar 

  • Tedder RS, Mortimer PP, Lord RB (1981) Detection of antibody to varicella-zoster virus by competitive and IgM-antibody capture immunoassay. J Med Virol 8: 89–101

    PubMed  CAS  Google Scholar 

  • Traavik T (1982) A simple and rapid method for detection of serum IgM antibodies to the rubella virus hemagglutinin. J Virol Methods 3: 337–347

    Article  PubMed  CAS  Google Scholar 

  • Trent DW, Harvey CL, Qureshi A, LeStourgeon D (1976) Solid-phase radioimmunoassay for anti-bodies to flavivirus structural and nonstructural proteins. Infect Immun 13: 1325–1333

    PubMed  CAS  Google Scholar 

  • Uhr JW, Finkelstein MS (1963) Antibody formation. IV. Formation of rapidly and slowly sedi-menting antibodies and immunological memory to bacteriophage ΦX174. J Exp Med 117: 457–477

    Article  PubMed  CAS  Google Scholar 

  • Ukkonen P, Penttinen K (1981) Immunity induced by formalin-inactivated mumps virus vaccine: suppression of IgM antibody response. J Infect Dis 142: 496–497

    Article  Google Scholar 

  • Ukkonen P, Väisänen O, Penttinen K (1980) Enzyme-linked immunosorbent assay for mumps and parainfluenza type 1 immunoglobulin G and immunoglobulin M antibodies. J Clin Microbiol 11: 319–323

    PubMed  CAS  Google Scholar 

  • Ukkonen P Grandström M-L, Penttinen K (1981) Mumps-specific immunoglobulin M and G anti-bodies in natural mumps infection as measured by enzyme-linked immunosorbent assay. J Med Virol 8: 131–142

    Google Scholar 

  • Urquhart GED (1974) Serum IgM and IgA responses in influenza A infections. J Clin Pathol 27: 198–201

    Article  PubMed  CAS  Google Scholar 

  • Van der Logt JTM, van Loon AM, van der Veen J (1981) Hemadsorption immunosorbent technique for determination of rubella immunoglobulin M antibody. J Clin Microbiol 13: 410–415

    PubMed  Google Scholar 

  • Van der Logt JTM, Heessen FWA, van Loon AM, van der Veen J (1982) Hemadsorption immunosorbent technique for determination of mumps immunoglobulin M antibody. J Clin Microbiol 15: 82–86

    PubMed  Google Scholar 

  • Van Loon AM, Heessen FWA, van der Logt JTM, van der Veen J (1981a) Direct enzyme-linked immunosorbent assay that uses peroxidase-labeled antigen for determination of immune-globulin M antibody to cytomegalovirus. J Clin Microbiol 13: 416–422

    PubMed  Google Scholar 

  • Van Loon AM, van der Logt JTM, van der Veen J (1981b) Diagnosis of herpes encephalitis by ELISA. Lancet 2: 1228–1229

    Article  PubMed  Google Scholar 

  • Vathanophas K, Hammon WMcD, Atchison RW, Sather GE (1973) Attempted type-specific diagnosis of dengue virus infection by the indirect fluorescent antibody method directed at differentiating IgM and IgG responses. Proc Soc Exp Biol Med 142: 697–702

    PubMed  CAS  Google Scholar 

  • Vejtorp M (1980) The interference of IgM rheumatoid factor in enzyme-linked immunosorbent assays of rubella IgM and IgG antibodies. J Virol Methods 1: 1–9

    Article  CAS  Google Scholar 

  • Vejtorp M (1981) Solid-phase anti-IgM ELISA for detection of rubella-specific IgM antibodies. Acta Pathol Microbiol Scand [B] 89: 123–128

    CAS  Google Scholar 

  • Vejtorp M, Fanøe E, Leerhoy J (1979) Diagnosis of postnatal rubella by the enzyme-linked immunosorbent assay for rubella IgM and IgG antibodies. Acta Pathol Microbiol Scand [B] 87: 155–160

    Google Scholar 

  • Vesikari T, Vaheri A (1968) Rubella: a method for rapid diagnosis of a recent infection by demonstration of the IgM antibodies. Br Med J 1: 221–223

    Article  PubMed  CAS  Google Scholar 

  • Voller A, Bidwell DE (1976) Enzyme-immunoassays for antibodies in measles, cytomegalovirus infections and after rubella vaccination. Br J Exp Pathol 57: 243–247

    PubMed  CAS  Google Scholar 

  • Vuorimaa TO, Arstila PP, Ziola BR, Salmi AA, Hanninen PT, Halonen PE (1978) Solid-phase radioimmunoassay determination of virus-specific IgM antibody levels in a follow-up of patients with naturally acquired measles infections. J Med Virol 2: 271–278

    Article  PubMed  CAS  Google Scholar 

  • Wager O, Räsänen JA, Hagman A, Klemola E (1968) Mixed cryoimmunoglobulinaemia in infec-tious mononucleosis and cytomegalovirus mononucleosis. Int Arch Allergy Appl Immunol 34: 345–361

    Article  PubMed  CAS  Google Scholar 

  • Welliver RC, Kaul TN, Putnam TI, Sun M, Riddlesberger K, Ogra PL (1980) The antibody response to primary and secondary infection with respiratory syncytial virus: kinetics of class-specific responses. J Pediatr 96: 808–813

    Article  PubMed  CAS  Google Scholar 

  • Westaway EG, Della-Porta AJ, Reedman BM (1974) Specificity of IgM and IgG antibodies after challenge with antigenically related togaviruses. J Immunol 112: 656–r663

    PubMed  CAS  Google Scholar 

  • Wolf HJ, Frosner GG, Deinhardt F (1979) Method for rapid separation of immunoglobulin M from immunoglobulin G antibodies by using reorienting gradients in vertical rotors. J Clin Microbiol 9: 544–r546

    PubMed  CAS  Google Scholar 

  • Wolff KL, Muth DJ, Hudson BW, Trent DW (1981) Evaluation of the solid-phase radioimmunoassay for diagnosis of St. Louis encephalitis infection in humans. J Clin Microbiol 14:135–r 140

    Google Scholar 

  • Yolken RH, Leister FJ (1981) Enzyme immunoassays for measurement of cytomegalovirus immunoglobulin M antibody. J Clin Microbiol 14: 427–r432

    PubMed  CAS  Google Scholar 

  • Yolken RH, Wyatt RG, Kim HW, Kapikian AZ, Chanock RM (1978) Immunological response to infection with human reovirus-like agent: measurement of anti-human reovirus-like agent immunoglobulin G and M levels by the method of enzyme-linked immunosorbent assay. Infect Immun 19: 540–r546

    PubMed  CAS  Google Scholar 

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  1. Department of Virology, University of Turku, 20520, Turku 52, Finland

    Olli Meurman

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    Peter A. Bachmann

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Meurman, O. (1983). Detection of Antiviral IgM Antibodies and its Problems — A Review. In: Bachmann, P.A. (eds) New Developments in Diagnostic Virology. Current Topics in Microbiology and Immunology, vol 104. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-68949-9_7

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