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Restriction Endonuclease Patterns of Herpes Simplex Virus DNA: Application to Diagnosis and Molecular Epidemiology

  • Chapter
New Developments in Diagnostic Virology

Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 104))

Abstract

Historically, the indications that herpes simplex virus (HSV) strains may vary considerably emerged many years ago (Ashe and Scherp 1965; Hampar and Burroughs 1969). For one of us (BR), the studies on the variability of HSV began with a chance isolation of a mutant [HSV-1 (MP)] that fused cells into polykaryocytes from a wild type virus [HSV-l (mP)] which caused cells to round and clump (Hoggan and Roizman 1959). Subsequent studies showed that the parent and mutant differed with respect to buoyant density in CsCl gradients (Roizman and Roane 1961), affinity chromatography (Roizman and Roane 1963), neutralization with specific antisera (Roizman and Roane 1963), the ability to accumulate glycoprotein VP8(gC) (Heine et al. 1974) and, ultimately, with respect to several genetic loci concerned with cell fusion in addition to accumulation of glycoprotein VP8(gC) (Ruyechan et al. 1979). The discovery that HSV represents two viruses, HSV-1 and HSV-2 (Schneweiss 1962; Dowdle et al. 1967), and studies on isolates and mutants of each (Ejercito et al. 1968; Heine et al. 1974; Seth etal. 1974; Terni and Roizman 1970), reinforced the hypothesis that HSV strains vary in a number of characteristics.

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References

  • Adams G, Stoves BH, Keenlyside RA, Hooton TM, Buchman TG, Roizman B, Stewart JA (1981) Nosocomial herpetic infections in a pediatric intensive care unit. Am J Epidemiol 113: 126–132

    PubMed  CAS  Google Scholar 

  • Ashe WK, Scherp HW (1965) Antigenic variations in herpes simplex virus isolates from successive recurrences of herpes labialis. J Immunol 94: 385–394

    PubMed  CAS  Google Scholar 

  • Buchman TG, Roizman B, Adams G, Stover BH (1978) Restriction endonuclease fingerprinting of herpes simplex virus DNA: a novel epidemiological tool applied to a nosocomial outbreak. J Infect Dis 138: 488–498

    Article  PubMed  CAS  Google Scholar 

  • Buchman TG, Roizman B, Nahmias AJ (1979) Exogenous genital reinfection with herpes simplex virus 2 demonstrated by restriction endonuclease fingerprinting of viral DNA. J Infect Dis 140: 295–304

    Article  PubMed  CAS  Google Scholar 

  • Buchman TG, Simpson T, Nosal C, Roizman B, Nahmias AJ (1980) The structure of herpes simplex virus DNA and its application to molecular epidemiology. Ann NY Acad Sci 354: 279–290

    Article  PubMed  CAS  Google Scholar 

  • Centifanto-Fitzgerald YM, Varnell ED, Kaufman HE (1982) Initial herpesvirus type 1 infection prevents ganglionic superinfection by another strain. Infect Immun 35: 1125–1132

    PubMed  CAS  Google Scholar 

  • Dambaugh T, Raab-Traub N, Heller M, Beisel C, Hummel M, Cheung A, Fennewald S, King W, Kieff E (1980) Variation among isolates of Epstein-Barr virus. Ann NY Acad Sci 354: 309–325

    Article  PubMed  CAS  Google Scholar 

  • Dowdle WR, Nahmias AJ, Harwell RW, Pauls FP (1967) Association of antigenic type of Herpesvirus hominis with site of viral recovery. J Immunol 99: 974–980

    PubMed  CAS  Google Scholar 

  • Ejercito PM, Kieff ED, Roizman B (1968) Characterization of herpes simplex virus strains differing in their effects on social behavior of infected cells. J Gen Virol 2: 357–364

    Article  PubMed  CAS  Google Scholar 

  • Goodheart CR, Plummer G, Waner JL (1968) Density difference of DNA of human herpes simplex viruses types I and II. Virology 35: 473–475

    Article  PubMed  CAS  Google Scholar 

  • Halperin SA, Hendley JO, Nosal C, Roizman B (1980) DNA fingerprinting in investigation of apparent nosocomial acquisition of neonatal herpes simplex. J Pediatr 97: 91–93

    Article  PubMed  CAS  Google Scholar 

  • Hammer SM, Buchman TG, D’Angelo LJ, Karchmer AW, Roizman B, Hirsch MS (1980) Temporal cluster of herpes simplex encephalitis: investigation by restriction endonuclease cleavage of viral DNA. J Infect Dis 141: 436–440

    Article  PubMed  CAS  Google Scholar 

  • Hampar B, Burroughs MAK (1969) Mechanism of persistent herpes simplex virus infection in vitro. J Natl Cancer Inst 43: 621–634

    PubMed  CAS  Google Scholar 

  • Hayward GS, Frenkel N, Roizman B (1974) Anatomy of herpes simplex virus DNA: strain differences and heterogeneity in the locations of restriction endonuclease cleavage sites. Proc Natl Acad Sci USA 72: 1768–1772

    Article  Google Scholar 

  • Hayward GS, Jacobs RJ, Wadsworth SC, Roizman B (1975) Anatomy of herpes simplex virus DNA: evidence for four populations of molecules that differ in the relative orientation of their long and short segments. Proc Natl Acad Sci USA 72: 4243–4247

    Article  PubMed  CAS  Google Scholar 

  • Heine JW, Honess RW, Cassai E, Roizman B (1974) Proteins specified by herpes simplex virus. XII. The virion polypeptides of type 1 strains. J Virol 14: 640–651

    PubMed  CAS  Google Scholar 

  • Hoggan MD, Roizman B (1959) The isolation and properties of a variant of herpes simplex producing multinucleated giant cells in monolayer cultures in the presence of antibody. Am J Hyg 70: 208–219

    PubMed  CAS  Google Scholar 

  • Huang ES, Huong SM, Tegmeier GE, Alford C (1980) Cytomegalovirus: genetic variation of viral genomes. Ann NY Acad Sci 354: 332–346

    Article  PubMed  CAS  Google Scholar 

  • Kaerner HC, Ott-Hartmann A, Schatten R, Schroder CH, Gray CP (1981) Amplification of a short nucleotide sequence in the repeat units of defective herpes simplex virus type 1 Angelotti DNA. J Virol 39: 75–81

    PubMed  CAS  Google Scholar 

  • Kieff ED, Bachenheimer SL, Roizman B (1971) Size, composition, and structure of the deoxyribonucleic acid of herpes simplex virus subtypes 1 and 2. J Virol 8: 125–132

    PubMed  CAS  Google Scholar 

  • Kieff ED, Hoyer B, Bachenheimer S, Roizman B (1972) Genetic relatedness of type 1 and type 2 herpes simplex viruses. J Virol 9: 738–745

    PubMed  CAS  Google Scholar 

  • Linnemann CC Jr, Buchman TG, Light IJ, Ballard JL, Roizman B (1978) Transmission of herpes simplex virus type 1 in a nursery for the newborn: identification of viral isolates by DNA “fingerprinting.” Lancet 1: 964–966

    Article  PubMed  Google Scholar 

  • Locker H, Frenkel N (1979) Baml, Kpnl and Sail restriction enzyme maps of the DNAS of herpes simplex virus strains Justin and F: occurrence of heterogeneities in defined regions of the viral DNA. J Virol 32: 424–441

    Google Scholar 

  • Lonsdale DM, Brown SM, Subak-Sharpe JH, Warren KG, Koprowski H (1979) The polypeptide and the DNA restriction enzyme profiles of spontaneous isolates of herpes simplex virus type 1 from explants of human trigeminal, superior cervical, and vagus ganglia. J Gen Virol 43:151– 171

    Article  PubMed  CAS  Google Scholar 

  • Marsden HS, Stow ND, Preston VG, Timbury MC, Wilkie NM (1978) Physical mapping of herpes simplex virus induced polypeptides. J Virol 28: 624–642

    PubMed  CAS  Google Scholar 

  • Mocarski ES, Roizman B (1981) Site-specific inversion sequence of the herpes simplex virus genome: domain and structural features. Proc Natl Acad Sci USA 78: 7047–7051

    Article  PubMed  CAS  Google Scholar 

  • Mocarski ES, Post LE, Roizman B (1980) Molecular engineering of the herpes simplex virus genome: insertion of a second L-S junction into the genome causes additional genome inversions. Cell 22: 243–255

    Article  PubMed  CAS  Google Scholar 

  • Morse LS, Buchman TG, Roizman B, Schaffer PA (1977) Anatomy of herpes simplex virus DNA. IX Apparent exclusion of some parental DNA arrangement in the generation of intertypic (HSV-1 X HSV-2) recombinants. J Virol 24: 231–248

    PubMed  CAS  Google Scholar 

  • Palese P, Roizman B (eds) (1980) Genetic variation of viruses. Ann NY Acad Sci 354: 1–507

    Google Scholar 

  • Pereira L, Cassai E, Honess RW, Roizman B, Terni M, Nahmias AJ (1976) Variability in the structural polypeptides of herpes simplex virus 1 strains: potential application in molecular epidemiology. Infect Immun 13: 211–220

    PubMed  CAS  Google Scholar 

  • Roizman B (1979) The structure and isomerization of herpes simplex virus genomes. Cell 16:481– 494

    Article  PubMed  CAS  Google Scholar 

  • Roizman B, Roane PR Jr (1961) A physical difference between two strains of herpes simplex virus apparent on sedimentation in cesium chloride. Virology 15: 75–79

    Article  PubMed  CAS  Google Scholar 

  • Roizman B, Roane PR Jr (1963) Demonstration of a surface difference between virions of two strains of herpes simplex virus. Virology 19: 198–204

    Article  PubMed  CAS  Google Scholar 

  • Roizman B, Tognon M (1982) Restriction enzyme analysis of herpesvirus DNA: stability of restriction endonuclease patterns. Lancet, pp 677

    Google Scholar 

  • Roizman B, Hayward G, Jacob R, Wadsworth SW, Honess RW (1974) Human herpesvirus 1: a model for molecular organization of herpesvirus virions and their DNA. In: Chemical and viral carcinogenesis, vol 2. Proceedings of the 11th international congress on cancer. Excerpta Medica, Amsterdam, pp 188–198

    Google Scholar 

  • Roizman B, Kozak M, Honess RW, Hayward G (1979) Regulation of herpesvirus macromolecular synthesis: evidence for multilevel regulation of herpes simplex 1 RNA and protein synthesis. Cold Spring Harbor Symp Quant Biol 39: 687–702

    Google Scholar 

  • Ruyechan WT, Morse LS, Knipe DM, Roizman B (1979) Molecular genetics of herpes simplex virus. II. Mapping of the major virus glycoproteins and of the genetic loci specifying the social behavior of infected cells. J Virol 29: 677–697

    PubMed  CAS  Google Scholar 

  • Sabine M, Robertson GR, Whalley JM (1981) Differentiation of subtypes of equine herpesvirus 1 by restriction endonuclease analysis. Aust Vet J 57: 148–149

    Article  PubMed  CAS  Google Scholar 

  • Schneweiss KE (1962) Serologische Untersuchungen zur Typendifferenzierung des Herpesvirus hominis. Z Immunitaetsforsch 124: 24–48

    Google Scholar 

  • Seth P, Rawls WE, Duff R, Rapp F, Adam E, Melnick JL (1974) Antigenic differences between isolates of herpesvirus type 2. Intervirology 3: 1–14

    Article  PubMed  CAS  Google Scholar 

  • Sheldrick B, Berthelot N (1975) Inverted repetitions in the chromosome of herpes simplex virus. Cold Spring Harbor Symp Quant Biol 39: 667–668

    PubMed  Google Scholar 

  • Skare J, Summers WP, Summers WC (1975) Structure and function of herpesvirus genomes. I. Comparison of the five HSV-1 and two HSV-2 strains by cleavage of their DNA with EcoK restriction endonuclease. J Virol 15: 726–732

    PubMed  CAS  Google Scholar 

  • Studdert MJ, Simpson T, Roizman B (1981) Differentiation of respiratory and abortigenic isolates of equine herpesvirus 1 by restriction endonucleases. Science 214: 562–564

    Article  PubMed  CAS  Google Scholar 

  • Terni M, Roizman B (1970) Variability of herpes simplex virus: isolation of two variants from simultaneous eruption at different sites. J Infect Dis 121: 212–216

    Article  PubMed  CAS  Google Scholar 

  • Turtinen LW, Allen GP, Darlington RW, Bryans JT (1981) Serologic and molecular comparisons of several equine herpesvirus type 1 strains. Am J Vet Res 42: 2099–2104

    PubMed  CAS  Google Scholar 

  • Wadsworth S, Jacob RJ, Roizman B (1975) Anatomy of herpes simplex virus DNA. II. Size, composition and arrangement of inverted terminal repetitions. J Virol 15: 1487–1497

    PubMed  CAS  Google Scholar 

  • Wagner MM, Summers WC (1978) Structure of the joint region and termini of the DNA of herpes simplex virus type 1. J Virol 27: 374–387

    PubMed  CAS  Google Scholar 

  • Whitley R, Nahmias A, Roizman B (to be published) Differentiation by DNA restriction analysis of herpes simplex virus isolates obtained concomitantly from the brain and orolabial sites of patients with encephalitis. N Engl J Med

    Google Scholar 

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Author information

Authors and Affiliations

  1. Marjorie B. Kovler Viral Oncology Laboratories, The University of Chicago, Chicago, Ill., 60637, USA

    Bernard Roizman

  2. Institute of Microbiology, University of Ferrara, Ferrara, Italy

    Mauro Tognon

Authors
  1. Bernard Roizman
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  2. Mauro Tognon
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Editors and Affiliations

  1. Institut für Medizinische Mikrobiologie, Infektions- und Seuchenmedizin, Veterinärstr. 13, 8000, München 22, Germany

    Peter A. Bachmann

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© 1983 Springer-Verlag Berlin Heidelberg

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Roizman, B., Tognon, M. (1983). Restriction Endonuclease Patterns of Herpes Simplex Virus DNA: Application to Diagnosis and Molecular Epidemiology. In: Bachmann, P.A. (eds) New Developments in Diagnostic Virology. Current Topics in Microbiology and Immunology, vol 104. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-68949-9_17

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  • DOI: https://doi.org/10.1007/978-3-642-68949-9_17

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-68951-2

  • Online ISBN: 978-3-642-68949-9

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