Abstract
Utilizing normal patient blood samples, two automated differential pattern recognition systems, the ADC-500 and the Hematrak 360, were compared in a duplicate study with the manual eye method, using spun and wedge smear preparations. The study showed a superior precision for the 500-cell differential, but occasional data points with spun smears were unusually drifted from the central cluster for both the manual and instrument methods. Analysis of the data uncovered at least two reasons for these unusual outliers: “star artifacts” and warped glass slides. The importance of these method-related artifacts are discussed, along with the future of the 500-cell differential with today’s automated technology.
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References
CRUICKSHANK, J.M., ALEXANDER, W.K.: The effect of age, sex, parity, haemoglobin level, and oral contraceptive preparations on the normal leucocyte count. Br. J. Hematol. 18, 541, 1970
DAVIDSON, E.: The distribution of cells in peripheral blood smears. J. Clin. Pathol. 11, 410, 1958
ENGLAND, J.M., BAIN, B.J.: Total and differential leukocyte count. Br. J. Hematol. 33, 1, 1976
GOLDNER, F.M., MANN, W.N.: The statistical error of the differential leukocyte count. Guys Hosp. Rep. 88, 54, 1938
KOEPKE, J.A.: A delineation of performance criteria for the differentiation of leukocytes. Am. J. Clin. Pathol. 68, 202, 1977
MACGREGOR, R.G.S., RICHARDS, W., LOH, G.L.: The differential leucocyte count. J. Pathol. 51, 337, 1940
ORFANEKIS, N.G., OSTLUND, R.E., BISHOP, C.R., ATHENS, J.W.: Normal blood leukocyte concentration values. Am. J. Clin. Pathol. 53, 647, 1970
RÜMKE, C.L.: Variability of results in differential cell counts on blood smears. Triangle 4, 154, 1960
SCHLEICHER, E.M.: Technique for determination of the differential leukocyte ratio and total leukocyte count. Minn. Med. 45, 914, 1962
SIMMONS, A., LEAVERTON, P., HILDEBRANT, J.: Factors affecting manual white cell differential counts. Am. J. Med. Technol. 39, 354, 1973
STATLAND, B.E., WINKEL, P., HARISS, S.C., BURDSALL, M.J., SAUNDERS, A.M.: Evaluation of biologic sources of variation of leukocyte counts. Am. J. Clin. Pathol. 69, 48, 1978
WENKE, R.E.: Comparison of five methods for preparing blood smears. Am. J. Med. Technol. 42, 71, 1976
WINTROBE, M.M.: Clinical Hematology. Philadelphia, Lea & Febiger, 1974
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© 1981 Springer-Verlag Berlin Heidelberg
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Kingsley, T.C. (1981). The Automated Differential; Pattern Recognition Systems, Precision, and the Spun Smear. In: Ross, D.W., Brecher, G., Bessis, M. (eds) Automation in Hematology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67756-4_28
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DOI: https://doi.org/10.1007/978-3-642-67756-4_28
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-10225-0
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