Zusammenfassung
Im Zellkern der Eukaryonten findet man eine extrem hohe Transkriptionsrate, aber nur ein geringer Prozentsatz der gebildeten RNS verläßt den Kern, wandert an die Ribosomen im Cytoplasma und wirkt als Messenger. Der Rest ist kurzlebig (t1/2 = ~10–30 Min.), die Moleküle werden im Kern wieder degradiert. Sie sind unterschiedlich lang und enthalten oft viele tausend Nukleotide; die Sedimentationskonstanten nehmen Werte bis zu 40–50 S an. Man nennt diese Molekülfraktion daher hnRNS (heterogene RNS). Für uns stellen sich damit die Fragen:
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Woher stammt die mRNS?
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Ist sie ein Abbauprodukt der hnRNS, oder wird sie unabhängig von ihr gebildet?
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Literatur
Bishop, J.O., Morton, J.G., Rosbash, M., Richardson, M.: Three abundance classes in HeLa cell messenger RNA. Nature (London) 250, 199 (1974)
Bishop, J.O., Beckmann, J.S., Campo, M.S., Hastie, N.D., Izquierdo, M., Perlman, S.: DNA-RNA hybridization. Philos. Trans. R. Soc. Lond. B 272, 147 (1975)
Brawerman, G.: Eukaryotic messenger RNA. Annu. Rev. Biochem. 43, 621 (1974)
Britten, R.J., Davidson, E.H.: Gene regulation for higher cells: a theory. Science 165, 349 (1969)
Brown, D.D., David, I.B.: Specific gene amplification in oocytes. Science 160, 272 (1968)
Campo, M.S., Bishop, J.O.: Two classes of messenger RNA in cultured rat cells: repetitive sequence transcripts and unique sequence transcripts. J. Mol. Biol. 90, 649 (1974)
Davidson, E.H., Klein, W.H., Britten, R.J.: Sequence organization in animal DNA and a speculation on hnRNA as a coordinate regulatory transcript. Dev. Biol. 55, 69 (1977)
Derman, E., Goldberg, S., Darnell, J.E.: hnRNA in HeLa cells: Distribution of transcript sizes estimated from nascent molecule profile. Cell 9, 465 (1976)
Galau, G.A., Britten, R.J., Davidson, E.H.: A measurement of the sequence complexity of polysomal messenger RNA in sea urchin embryos. Cell 2, 9 (1974)
Galau, G.A., Klein, W.H., Britten, R.J., Davidson, E.H.: Significance of rare mRNA sequences in liver. Arch. Biochem. Biophys. 179, 584 (1977)
Hunter, T., Garreis, J.I.: Characterization of the mRNAs for α-, ß- and γ-Actin. Cell 12, 767 (1977)
Klein, W.H., Murphy, W., Attardi, G., Britten, R.J., Davidson, E.H.: Distribution of repetitive and non-repetitive sequence transcripts in HeLa mRNA. Proc. Natl. Acad. Sci. USA 71, 1785 (1974)
Knapp, G., Beckmann, J.S., Johnson, P.F., Fuhrman, S.A., Abelson, J.: Transcription and processing of intervening sequences in yeast tRNA genes. Cell 14, 221 (1978)
Lewin, B.: Units of transcription and translation. The relationship between heterogeneous nuclear RNA and messenger RNA. Cell 4, 11 (1975)
Paul, J., Gilmour, R.S.: Organ-specific restriction of transcription in mammalian chromatin. J. Mol. Biol. 34, 305 (1968)
Proudfoot, N.J., Brownlee, G.G.: 3′ non-coding region sequences in eukaryotic messenger RNA. Nature (London) 263, 211 (1976)
Revel, M., Groner, Y.: Post-transcriptional and translational controls of gene expression in eukaryotes. Annu. Rev. Biochem. 47, 1079 (1978)
Sawicki, S.G., Jelinek, W., Darnell, J.E.: 3′-terminal addition to HeLa cell nuclear and cytoplasmic poly (A). J. Mol. Biol. 113, 219 (1977)
Sippel, A.E., Stavrianopoulos, J.G., Schutz, G., Feigelson, P.: Translational properties of rabbit globin mRNA after specific removal of poly (A) with ribonuclease H. Proc. Natl. Acad. Sci. USA 71, 4635 (1974)
Spradling, A., Penman, S., Campo, M.S., Bishop, J.O.: Repetitious and unique sequences in the heterogeneous nuclear and cytoplasmic messenger RNA of mammalian and insect cells. Cell 3, 23 (1974)
Whiteley, A.H., McCarthy, B.J., Whiteley, H.R.: Changing populations of messenger RNA during sea urchin development. Proc. Natl. Acad. Sci. USA 55, 519 (1966)
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von Sengbusch, P. (1979). Transkription der DNS eukaryotischer Zellen: hnRNS-mRNS. In: Molekular- und Zellbiologie. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67358-0_10
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