Abstract
Deutsch and Morton (1957) reported that sulfhydryl compounds, such as 2-mercaptoethanol, are able to dissociate IgM macroglobulins, thus reducing their 19 S sedimentation constants to 6.5 S, The reactivity of the IgM with the corresponding antigens is also abolished. The breakdown products of the dissociated macroglobulins will be recombined if the 2-mercaptoethanol is removed by dialysis, but the conformation of the reaggregated macromolecules is said to be different from the native globulin. This recombination may be prevented by the addition of SH-blocking agents such as iodoacetate to the phosphate buffer in which the dialysis of the 2-mercaptoethanol and macroglobulin mixture is carried out.
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References
Adler, Frank L.: J. Immunol. 95, 39 (1965)
Deutsch, H. F., and J. I. Morton: Science 125, 600 (1957)
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© 1979 Springer-Verlag Berlin Heidelberg
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Nowotny, A. (1979). Dissociation of Macroglobulins with 2-Mercaptoethanol. In: Basic Exercises in Immunochemistry. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67356-6_4
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DOI: https://doi.org/10.1007/978-3-642-67356-6_4
Publisher Name: Springer, Berlin, Heidelberg
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