Isolation of Immunoglobulins by Gel Filtration on Sephadex G 200 Column

Abstract

In contrast to adsorption, to distribution, or to ion exchange Chromatographic procedures, Sephadex gel chromatography or gel filtration fractionates components of a mixture on the basis of their molecular size. These gels, which are able to function on this principle, are also called molecular sieves. Small molecules which may enter the pores of a highly cross-linked gel polymer, will be retained on the column, while large molecules will travel free in the liquid that fills the spaces between the swollen gel granules (void volume). For a detailed description of the gel filtration processes the students should consult specified literature (Flodin, 1962).