Abstract
The method described in Exercise No, 44 yields an insoluble dinitrophenyl (DNP) protein which is suitable for chemical analysis, but cannot be used for immunobiologic assays. Eisen (1964) prepared DNP protein derivatives which are readily soluble in water or saline using a mild procedure. Using dinitrophenyl sulfonate instead of dinitrofluorobenzene, more selective dinitrophenylation could be achieved. This reagent is water soluble; its removal requires simple dialysis (or gel filtration). The number of DNP groups introduced on a protein can be measured photometrically.
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References
Eisen, H.: Meth. med. Res. 10, 94 (1964)
Eisen, H.: Meth. med. Res. 10, 106 (1964)
Karush, F.: J. Amer. Chem. Soc. 72, 2705 (1950)
Karush, F.: J. Amer. Chem. Soc. 72, 2714 (1950)
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Nowotny, A. (1979). Preparation of Soluble Dinitrophenyl Proteins. In: Basic Exercises in Immunochemistry. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67356-6_15
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DOI: https://doi.org/10.1007/978-3-642-67356-6_15
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