Abstract
Fly cells maintained in vitro would be useful for the study of fly-virus-man interactions. Ideally, these cells should come from an established tissue culture line. We have thus far had no success in establishing such a line, but one of us (Greenberg, 1969) had used tissues of Musca domestica and Musca sorbens in a preliminary study. Now we have been able to consistently obtain primary cultures from pupae of the fly, Sarcophaga bullata. These pupae are large — about 4 mm by 12 mm, and each weighs about 0.17 g, when the maggots are well nourished — so that a single animal can provide a sufficient number of cells to start a single culture. Microscopic techniques are not required.
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© 1971 Springer-Verlag, Berlin · Heidelberg
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Shinedling, S.T., Greenberg, B. (1971). Culture of Cells of the Flesh Fly, Sarcophaga bullata. In: Weiss, E. (eds) Arthropod Cell Cultures and Their Application to the Study of Viruses. Current Topics in Microbiology and Immunology, vol 55. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-65224-0_2
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DOI: https://doi.org/10.1007/978-3-642-65224-0_2
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