Abstract
A complete understanding of the function of RNA molecules requires a knowledge of their higher order structure (secondary and tertiary) as well as the characteristics of the sequence (primary structure). The two- and three-dimensional structure of RNA is important for many functions, including regulation of transcription and translation, catalysis, and transport of proteins across membranes. Molecules with the same function have the potential to fold into similar structures although they might differ in primary structure, a fact that helps to illustrate the importance of secondary and tertiary structure in relation to function. Examples of such constancy in secondary structure exist in tRNAs, 5sRNAs, 16sRNAs and viroid RNAs. Secondary and tertiary structure of tRNAphe (Kim et al. 1974) and of a hammerhead ribozyme (Pley et al. 1994) have been shown by their crystal structure. Currently little is known of tertiary interactions, but studies on tRNA indicate these are weaker than secondary structure interactions (Riesner and Romer 1973; Crothers and Cole 1978; Jaeger et al. 1990). It is however very difficult to crystallize and/or get nuclear magnetic resonance spectrum data for large RNA molecules. Therefore, a logical place to start in determining the 3-D structure of RNA is determination of the secondary structure.
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Currey, K.M., Shapiro, B.A. (1997). Higher Order Structures of Coxsackievirus B 5’ Nontranslated Region RNA. In: Tracy, S., Chapman, N.M., Mahy, B.W.J. (eds) The Coxsackie B Viruses. Current Topics in Microbiology and Immunology, vol 223. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60687-8_8
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DOI: https://doi.org/10.1007/978-3-642-60687-8_8
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