Abstract
Obtaining pure RNA is an essentia step in the analysis of patterns of gene expression and und the mechanism of gene expression.Thus, isolation of pure, intact RNA is one of the central techniques in molecular biology and represents an important step in Northern analysis, nuclease protection assays, RNA mapping, RT-PCR, cDNA library construction and in vitro translation experiments.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Asubel FM, Brent R, Kingston RG, Moor DD, Seidman JG, Smith JA and Struhl K (1987) Current protocols in molecular biology. Wiley & Sons Interscience. New York.
Berger SL, Birkenmeir CS (1979) X Biochemis try 18:51 43–5149.
Bugos RC, Chiang VL, Zhang X.-H, Campbell ER, Podilla GK, Campbell WH (1995) RNA isolation from plan t tissues recalcitrant to extraction in guanidine. BioTechniques 19:734–737.
Chirgwin J, Przybylska A, MacDonald R, Rutter W (1979) Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18:5294–5299.
Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:154–159.
Chomczynski P (1993) Areagent for the single-step simultaneous isolation ofRNA,DNA and proteins from cell and tissue samples. BioTechniques 15:532–537.
Chomczynski P, Mackey K (1995) Modification of the TRI Reagent“ procedure for isolation of RNA from polysaccharide-and proteoglycan-rich sources. BioTechniques 19:942–945.
Davis L, Kuehl M, Battey J (1994) Basic method on molecular biology. 2nd edition. Appelton & Lang, Norwalk, CT.
Hallick RB,Chelm BK,Gray PW, Orozco EM (1977) Use of aurintricarboxylic acid as an inhibitor of nucleases during nucleic acid isolation. Nucleic Acids Res. 4:3055–3064.
Kormanec J, Parkasovsk M (1994) Isolation of total RNA from yeast and bacteria and detection of rRNA in Northern blots. BioTechniques; 17:840–841.
Marcus L, Halvorson HO (1967) Resolution and isolation of yeast polysomes. In:Method in Enzymology 12A:498–502.Grossman L,Moldave K, Eds. Academic Press 1967.New York and London.
Noonberg SB, Scott GK, Benz C (1995) Effect of pH on RNA degradation during guanidinium extraction. BioTechniques 19:731–733.
Smith TJ (1998) Methods to remove DNA contamination from RNA samples. Ambion“ TechNotes™ from The RNA CompanyTM5:14–15. Ambion Inc. 1998.
Schultz DJ, Craig R, Cox-Foster DL, Mumma RO, Medford JI (1994) RNAisolation from recalcitrant plant tissue. Plant Molecular Biology Reporter. 12:310–316.
Rights and permissions
Copyright information
© 2000 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Surzycki, S. (2000). Isolation and Purification of RNA. In: Basic Techniques in Molecular Biology. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-56968-5_6
Download citation
DOI: https://doi.org/10.1007/978-3-642-56968-5_6
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-66678-3
Online ISBN: 978-3-642-56968-5
eBook Packages: Springer Book Archive