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Preparation of Genomic DNA from Animal Cells

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Basic Techniques in Molecular Biology

Part of the book series: Springer Lab Manuals ((SLM))

Abstract

This chapter describes three procedures for the isolation of anima DNA,one large scale procedure and two small scale procedures. All ofthese methods yield DNA suitable for restriction enzyme digestion, genomic library construction, Southern blot analysis and PCR. The large scale method will yield DNA in excess of 200 kb, whereas the small scale protocols will generate DNA of 50-150 kb.

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References

  • Chomczynski P, Mackey K, Drews R, Wilfinger W 1997 DNAz01®: A reagent for the rapid isolation of genomic DNA. BioTechniques 22:550–553.

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© 2000 Springer-Verlag Berlin Heidelberg

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Surzycki, S. (2000). Preparation of Genomic DNA from Animal Cells. In: Basic Techniques in Molecular Biology. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-56968-5_2

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  • DOI: https://doi.org/10.1007/978-3-642-56968-5_2

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-66678-3

  • Online ISBN: 978-3-642-56968-5

  • eBook Packages: Springer Book Archive

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