Abstract
This chapter describes three procedures for the isolation of anima DNA,one large scale procedure and two small scale procedures. All ofthese methods yield DNA suitable for restriction enzyme digestion, genomic library construction, Southern blot analysis and PCR. The large scale method will yield DNA in excess of 200 kb, whereas the small scale protocols will generate DNA of 50-150 kb.
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References
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© 2000 Springer-Verlag Berlin Heidelberg
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Surzycki, S. (2000). Preparation of Genomic DNA from Animal Cells. In: Basic Techniques in Molecular Biology. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-56968-5_2
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DOI: https://doi.org/10.1007/978-3-642-56968-5_2
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-66678-3
Online ISBN: 978-3-642-56968-5
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