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Photo-Convertible Reporters for Selective Visualization of Subcellular Events and Interactions

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Applied Plant Cell Biology

Part of the book series: Plant Cell Monographs ((CELLMONO,volume 22))

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Abstract

Photo-convertible fluorescent proteins are a recent addition to the cell biological tool box available to plant biologists. Here, we provide a brief overview of the use of green-to-red photo-convertible proteins such as monomeric EosFP, Kaede and Dendra2 for understanding subcellular behaviour in plants. A general description of the differential colouring technique using mEosFP for discriminating within an organelle population and tracking interactions between similar organelles is provided. This review aims to provide baseline standards and guidelines for pursuing studies using these novel proteins.

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Acknowledgments

We gratefully acknowledge the funding received from the Natural Sciences and Engineering Research Council (NSERC) and the Canada Foundation for Innovation (CFI) and the Canada and Ontario Ministry of Research and Innovation (OMRI) to JM and an NSERC Graduate scholarship to KB.

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Authors and Affiliations

  1. Laboratory of Plant Development and Interactions, Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road, Guelph, N1G2W1, Canada

    Kiah Barton, Alena Mammone & Jaideep Mathur

Authors
  1. Kiah Barton
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  2. Alena Mammone
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  3. Jaideep Mathur
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Correspondence to Jaideep Mathur .

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Editors and Affiliations

  1. Botanical Institute, University of Karlsruhe, Karlsruhe, Germany

    Peter Nick

  2. Charles University, Prague, Czech Republic

    Zdeněk Opatrny

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Barton, K., Mammone, A., Mathur, J. (2014). Photo-Convertible Reporters for Selective Visualization of Subcellular Events and Interactions. In: Nick, P., Opatrny, Z. (eds) Applied Plant Cell Biology. Plant Cell Monographs, vol 22. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-41787-0_14

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