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Automatic Annotation of Leishmania Infections in Fluorescence Microscopy Images

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Image Analysis and Recognition (ICIAR 2013)

Part of the book series: Lecture Notes in Computer Science ((LNIP,volume 7950))

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Abstract

Leishmania is a unicellular parasite that infects mammals. Biologists are interested in determining the effect of drugs in Leishmania infections. This requires the manual annotation of the number of macrophages and parasites in images, in order to obtain the percentage of infection (PI), the average number of parasites per infected cell (NPI) and the infection index (IX). Considering that manual annotation is tedious, time-consuming and often erroneous, in this paper we propose an automatic method for automatic annotation of Leishmania infections using fluorescence microscopy. Moreover, when compared to related works, the proposed method is able to get superior performance under most perspectives.

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References

  1. Fok, Y.L., Chan, J.C.K., Chin, R.T.: Automated analysis of nerve-cell images using active contour models. IEEE Transactions on Medical Imaging 15(3), 353–368 (1996)

    Article  Google Scholar 

  2. Kharma, N., Moghnieh, H., Yao, J., Guo, Y., Abu-Baker, A., Laganiere, J., Rouleau, G., Cheriet, M.: Automatic segmentation of cells from microscopic imagery using ellipse detection. IET Image Processing 1(1), 39–47 (2007)

    Article  Google Scholar 

  3. Faustino, G., Gattass, M., Rehen, S., de Lucena, C.: Automatic embryonic stem cells detection and counting method in fluorescence microscopy images. In: IEEE International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2009, July 1-28, pp. 799–802 (2009)

    Google Scholar 

  4. Nogueira, P.: Determining Leishmania Infection Levels by Automatic Analysis of Microscopy Images. Master’s thesis, Department of Computer Science, University of Porto (2011)

    Google Scholar 

  5. Nobuyuki, O.: A threshold selection method from gray-level histograms. IEEE Transactions on Systems, Man and Cybernetics 9(1), 62–66 (1979)

    Article  Google Scholar 

  6. Leal, P., Ferro, L., Marques, M., Romão, S., Cruz, T., Tomá, A.M., Castro, H., Quelhas, P.: Automatic assessment of leishmania infection indexes on in vitro macrophage cell cultures. In: Campilho, A., Kamel, M. (eds.) ICIAR 2012, Part II. LNCS, vol. 7325, pp. 432–439. Springer, Heidelberg (2012)

    Chapter  Google Scholar 

  7. Lindeberg, T.: Scale-Space Theory in Computer Vision. Kluwer Academic Publishers (1994)

    Google Scholar 

  8. Lindeberg, T.: Detecting salient blob-like image structures and their scales with a scale-space primal sketch: a method for focus-of-attention. Int. J. Comput. Vision 11(3), 283–318 (1993)

    Article  Google Scholar 

  9. Granlund, G.: Fourier preprocessing for hand print character recognition. IEEE Transactions on Computers C-21(2), 195–201 (1972)

    Article  MathSciNet  Google Scholar 

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Neves, J.C., Castro, H., Proença, H., Coimbra, M. (2013). Automatic Annotation of Leishmania Infections in Fluorescence Microscopy Images. In: Kamel, M., Campilho, A. (eds) Image Analysis and Recognition. ICIAR 2013. Lecture Notes in Computer Science, vol 7950. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-39094-4_70

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  • DOI: https://doi.org/10.1007/978-3-642-39094-4_70

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-39093-7

  • Online ISBN: 978-3-642-39094-4

  • eBook Packages: Computer ScienceComputer Science (R0)

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