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The Influence of Temperature and Carbon Source on Expression of Recombinant HBcAg

  • Conference paper
International Symposium on Biomedical Engineering and Medical Physics, 10-12 October, 2012, Riga, Latvia

Part of the book series: IFMBE Proceedings ((IFMBE,volume 38))

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Abstract

Biotechnological aspects in the field of recombinant protein production are analysed in this work, which includes the investigation of factors for the production of virus like particles (VLPs) formed by recombinant Hepatitis B virus core-antigen (HBcAg) in inducible bacterial (E. coli) expression system as well as purification of these particles by gelfiltration and ion-exchange chromatography. Biomass yield and VLP production level depended on the cultivation conditions such carbon source and temperature. As inductor for protein expression lactose was used.

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Authors and Affiliations

  1. Institute of General Chemical Engineering, Riga Technical University, Riga, Latvia

    K. Rugele & J. Vanags

  2. Latvian Biomedical Research and Study Centre, Riga, Latvia

    I. Bērza & A. Dišlers

  3. Department of Water Engineering and Technology, Riga Technical University, Riga, Latvia

    S. Larsson

Authors
  1. K. Rugele
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  2. I. Bērza
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  3. S. Larsson
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  4. A. Dišlers
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  5. J. Vanags
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Editor information

Editors and Affiliations

  1. , Biomedical Engineering and, Riga Technical University, Kalku str 1, Riga, 1658, Latvia

    Yuri Dekhtyar

  2. , Biomedical Engineering and, Riga Technical University, Kalku str. 1, Riga, 1658, Latvia

    Alexei Katashev

  3. , Biomedical Engineering and, Riga Technical University, Kalku str. 1, Riga, 1658, Latvia

    Linda Lancere

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© 2013 IFMBE

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Rugele, K., Bērza, I., Larsson, S., Dišlers, A., Vanags, J. (2013). The Influence of Temperature and Carbon Source on Expression of Recombinant HBcAg. In: Dekhtyar, Y., Katashev, A., Lancere, L. (eds) International Symposium on Biomedical Engineering and Medical Physics, 10-12 October, 2012, Riga, Latvia. IFMBE Proceedings, vol 38. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-34197-7_28

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  • DOI: https://doi.org/10.1007/978-3-642-34197-7_28

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-34196-0

  • Online ISBN: 978-3-642-34197-7

  • eBook Packages: EngineeringEngineering (R0)

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