Abstract
Transfer of phycobilisome-containing cyanobacteria from darkness to continuous light results in a typical chlorophyll a fluorescence induction that follows a pattern labeled as OJIPSMT. This pattern of fluorescence induction (FI) reflects changes in both photochemical and non-photochemical processes. We have focused on the slow S to M fluorescence rise that is dominant in cyanobacteria. We clearly observe the S-to-M fluorescence rise in the wild type (WT) cells of Synechocystis sp. (PCC 6803) in the presence of 1, 1′-dimethyl-3(3′4′-dichloro)-phenylurea (DCMU). This rise is fully suppressed by hyperosmotic glycine betaine that prevents the mobility of extramembrane phycobilisomes. The contribution of the State 2-to-State 1 transition in the S-to-M rise of WT cells was proven by changes in the 77 K emission spectra: the spectra at point O of FI (i.e., in State 2, with lower ratio of F685/F726) were characteristically different from those at point M (i.e., in State 1, with higher ratio of F685/F726). The S-M rise was totally missing in RpaC— mutant of Synechocystis sp. (PCC 6803) that is locked in the high-fluorescence State 1 and thus is unable to do state transitions. Moreover, RpaC-mutant showed quenching of phycobilin fluorescence during the S-M rise period. Taken together, these data suggest that the State 2 to State 1 transition is the dominant cause of the S to M fluorescence rise in cyanobacteria.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Emlyn-Jones D, Ashby MK, Mullineaux CW (1999) A Gene Required for the Regulation of Photosynthetic Light Harvesting in the Cyanobacterium Synechocystis 6803. Molecular. Microbiol. 33: 1050–1058
Joshua S, Mullineaux CW (2004) Phycobilisome Diffusion Is Required for Light-State Transitions in Cyanobacterial. Plant. Physiol. 135: 2112–2119
Kaňa R, Prásil O, Komárek O, Papageorgiou GC, Govindjee (2009) Spectral Characteristic of Fluorescence Induction in a Model Cyanobacterium, Synechococcus sp (PCC 7942). Biochim. Biophys. Acta-Bioenergetics 1787: 1170–1178
Li DH, Xie J, Zhao JQ, Xia AD, Li DH, Gong YD (2004) Light-Induced Excitation Energy Redistribution in Spirulina Platensis Cells: “Spillover” or “Mobile PBSs”? Biochim. Biophys. Acta-Bioenergetics 1608: 114–121
McConnell MD, Koop R, Vasil’ev S, Bruce D (2002) Regulation of the Distribution of Chlorophyll and Phycobilin-Absorbed Excitation Energy in Cyanobacteria. A Structure-Based Model for the Light State Transition. Plant. Physiol. 130: 1201–1212
Mullineaux CW, Emlyn-Jones D (2005) State Transitions: an Example of Acclimation to Low-Light Stress. Journal of Experimental Botany 56: 389–393
Papageorgiou G, Govindje (1968) Light-Induced Changes in Fluorescence Yield of Chlorophyll Alpha in Vivo.I. Anacystis Nidulans. Biophys. J. 8: 1299–1315
Papageorgiou GC, Tsimilli-Michael M, Stamatakis K (2007) The Fast and Slow Kinetics of Chlorophyll a Fluorescence Induction in Plants, Algae and Cyanobacteria: a Viewpoint. Photosynth. Res. 94: 275–290
Stamatakis K, Tsimilli-Michael M, Papageorgiou GC (2007) Fluorescence Induction in the Phycobilisome-containing Cyanobacterium Synechococcus sp PCC 7942: Analysis of the Slow Fluorescence Transient. Biochim. Biophys. Acta-Bioenergetics 1767: 766–772
Tsimilli-Michael M, Stamatakis K, Papageorgiou GC (2009) Dark-to-Light Transition in Synechococcus sp PCC 7942 Cells Studied by Fluorescence Kinetics Assesses Plastoquinone Redox Poise in the Dark and Photosystem II Fluorescence Component and Dynamics during State 2 to State I Transition. Photosynth. Res. 99: 243–255
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
Copyright information
© 2013 Zhejiang University Press, Hangzhou and Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Kaňa, R., Komárek, O., Kotabová, E., Papageorgiou, G.C., Govindjee, Prášil, O. (2013). The Slow S to M Fluorescence Rise is Missing in the RpaC Mutant of Synechocystis sp. (PCC 6803). In: Photosynthesis Research for Food, Fuel and the Future. Advanced Topics in Science and Technology in China. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-32034-7_104
Download citation
DOI: https://doi.org/10.1007/978-3-642-32034-7_104
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-32033-0
Online ISBN: 978-3-642-32034-7
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)