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Genome-Wide Analysis of Transposition Using Next Generation Sequencing Technologies

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Plant Transposable Elements

Part of the book series: Topics in Current Genetics ((TCG,volume 24))

Abstract

Transposable elements (TEs) make a large part of most eukaryotic genomes and strongly impact their structure, function, and evolution. The identification of active TEs in a genome is, therefore, essential in order to fully understand its dynamics at both structural and functional levels. The recent advent of new sequencing technologies, often referred to as next generation sequencing (NGS) technologies, has opened new doors to study structural variations at full genome scale. Although restricted so far mostly to human studies, these new strategies have shown to be highly efficient and promising in few other model species, including the two plant species Arabidopsis thaliana and rice. This chapter describes the concepts and techniques of using NGS for the study of TE activity in eukaryotic genomes at large.

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Authors and Affiliations

  1. Laboratoire Génome et Développement des Plantes, UMR UPVD/CNRS 5096, Université de Perpignan Via Domitia, 52, Avenue Paul Alduy, 66860, Perpignan cedex, France

    Moaine Elbaidouri & Olivier Panaud

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  1. Moaine Elbaidouri
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  2. Olivier Panaud
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Correspondence to Olivier Panaud .

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Editors and Affiliations

  1. , Institut Jean-Pierre Bourgin (IJPB), INRA Versailles, UMR 1318, Versailles Cedex, 78026, France

    Marie-Angèle Grandbastien

  2. , Centre de Recerca en Agrigenomica (CRAG), CSIC-RTA-UAB, Barcelona, 08193, Spain

    Josep M. Casacuberta

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Elbaidouri, M., Panaud, O. (2012). Genome-Wide Analysis of Transposition Using Next Generation Sequencing Technologies. In: Grandbastien, MA., Casacuberta, J. (eds) Plant Transposable Elements. Topics in Current Genetics, vol 24. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-31842-9_4

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