Abstract
Three-dimensional optical-resolution photoacoustic microscopy (OR-PAM), an emerging optical-acoustic hybrid technology capable of imaging optical absorption contrasts with subcellular resolution and sensitivity, has been recognized as a valuable complement to existing optical microscopy technologies. In this chapter, we provide detailed discussion on the design and operation of OR-PAM, including the principle, system design, system configuration, system alignment, experimental procedures, laser safety, functional imaging scheme, recent technical advances, and sample biomedical applications. Future directions of OR-PAM development are also discussed at the end of this chapter.
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Acknowledgements
This work was sponsored by National Institutes of Health Grants R01 EB000712, EB000712A2S1, R01 EB00071207S2, R01 EB008085, R01 CA113453901, U54 CA136398, and 5P60 DK02057933. Professor Lihong V. Wang has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.
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Hu, S., Maslov, K., Wang, L.V. (2013). Three-Dimensional Optical-Resolution Photoacoustic Microscopy. In: Liang, R. (eds) Biomedical Optical Imaging Technologies. Biological and Medical Physics, Biomedical Engineering. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-28391-8_2
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