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Intravital Multiphoton Imaging of Immune Cells

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Advances in Bio-Imaging: From Physics to Signal Understanding Issues

Part of the book series: Advances in Intelligent and Soft Computing ((AINSC,volume 120))

Abstract

Intravital multiphoton microscopy (MP-IVM) is a powerful imaging approach that allows direct visualization of cells within their native environment in real time. Multiphoton imaging of immune cells has been performed in different tissues, and these studies have revealed intriguing insights into the unique migratory and interactive behavior of immune cells in the steady-state and during disease conditions. Here we provide an overview of a MP-IVM model of the mouse ear skin, as well as the benefits, limitations and pitfalls of this approach. We also discuss the prospects of intravital imaging in the areas of image analysis, data management and standardization.

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Author information

Authors and Affiliations

  1. Singapore Immunology Network (SIgN), Agency for Science, Technology, and Research (A*STAR), Biopolis, Singapore

    Jo Keeble, Chi Ching Goh, Yilin Wang & Lai Guan Ng

  2. The Centenary Institute, Newtown, NSW, 2042, Australia

    Wolfgang Weninger

  3. Discipline of Dermatology, University of Sydney, Camperdown, NSW, 2050, Australia

    Wolfgang Weninger

  4. Australia & Department of Dermatology, Royal Prince Alfred Hospital, Camperdown, NSW, 2050, Australia

    Wolfgang Weninger

Authors
  1. Jo Keeble
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  2. Chi Ching Goh
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  3. Yilin Wang
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  4. Wolfgang Weninger
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  5. Lai Guan Ng
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Editor information

Editors and Affiliations

  1. IPAL, I2R & CNRS, 1 Fusionoplis Way #10-18, Singapore, 138632, Singapore

    Nicolas Loménie

  2. IPAL, I2R & CNRS, 1 Fusionoplis Way #21-01, Singapore, 138632, Singapore

    Daniel Racoceanu

  3. Singapore Immunology Network (SIgN), 8A Biomedical Grove, Immunos Building, Level 4, Singapore, 138648, Singapore

    Alexandre Gouaillard

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© 2012 Springer Berlin Heidelberg

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Keeble, J., Goh, C.C., Wang, Y., Weninger, W., Ng, L.G. (2012). Intravital Multiphoton Imaging of Immune Cells. In: Loménie, N., Racoceanu, D., Gouaillard, A. (eds) Advances in Bio-Imaging: From Physics to Signal Understanding Issues. Advances in Intelligent and Soft Computing, vol 120. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-25547-2_1

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  • DOI: https://doi.org/10.1007/978-3-642-25547-2_1

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-25546-5

  • Online ISBN: 978-3-642-25547-2

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