Abstract
PCR approach was used in order to investigate the presence of plant DNA in pellet of somatic cells from fresh or stored milk of twenty grazing goats fed concentrate (18% CP; 1.03 UFL/kg, as fed) containing conventional or genetically modified soybean meal. The quantity and the purity of DNAs extracted from fresh samples were higher than those of samples stored at −20°C (ng/μl: 51.6 vs 20.8; A260/A280: 1.7 vs 1.4). The frequency of chloroplast sequence, for both groups, ranged between 70 and 100% in fresh and between 30 and 40% in stored samples. The frequency percentage of specific soybean gene was lower in stored (25–33%) than in fresh samples (56–88%). Transgenic fragments were found only in milk from goats fed GM soybean. For 35S promoter, the frequency was 38–75% in fresh and 25% in stored samples, while for CP4 EPSPS, it was 38–50% in fresh and 25% in stored samples.
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Abbreviations
- CP:
-
Crude protein
- DM:
-
Dry matter
- GM:
-
Genetically modified
- s.e.:
-
Solvent extract
References
Bottero MT, Civera T, Nucera D, Rosati S, Sacchi P, Turi RM (2003) A multiplex polymerase chain reaction for the identification of cows’, goats’ and sheep’s milk in dairy products. Int Dairy J 13:277–282
Chowdhury EH, Mikami O, Nakajima Y, Kuribara H, Hino A, Suga K, Hanazumi M, Yomemochi C (2003) Detection of genetically modified maize DNA fragments in the intestinal contents of pigs fed StarLinkTM CBH351. Vet Hum Toxicol 45:95–96
EFSA (2008) Safety and nutritional assessment of GM plants and derived food and feed: the role of animal feeding trials. Food Chem Toxicol 46:S2–S70
Einspanier R, Klotz A, Kraft J, Aulrich K, Poser R, Schwägele F, Jahreis G, Flachowsky G (2001) The fate of forage plant DNA in farm animals: a collaborative case-study investigating cattle and chicken fed recombinant plant material. Eur Food Res Technol 212:129–134
Hernández M, Rodríguez-Lázaro D, Esteve T, Prat S, Pla M (2003) Development of melting temperature-based SYBR Green I polymerase chain reaction methods for multiplex genetically modified organism detection. Anal Biochem 323:164–170
Kuribara H, Shindo Y, Matsuoka T, Takubo K, Futo S, Aoki N, Hirao T, Ariyama H, Goda Y, Toyoda M, Hino A (2002) Novel reference molecules for quantification of genetically modified maize and soybean. J AOAC Int 85:1077–1089
Lipp M, Brodmann P, Pietsch K, Pauwels J, Anklam E (1999) IUPAC collaborative trial study of a method to detect genetically modified soy beans and maize in dried powder. J AOAC Int 82:923–928
Mafra I, Ferriera IMPLVO, Oliveira MBPP (2008) Food authentication by PCR-based methods. Eur Food Res Technol 227:649–665
Phipps RH, Deaville ER, Maddison BC (2003) Detection of transgenic and Endogenous plant DNA in rumen fluid, duodenal digest, milk, blood, and feces of lactating dairy cows. J Dairy Sci 86:4070–4078
Poms RE, Hochsteiner W, Luger K, Glössl J, Foissy H (2003) Increased sensitivity for detection of specific target DNA in milk by concentration in milk fat. J Food Prot 66:304–310
Rizzi A, Bruseti L, Arioli S, Nielsen KM, Tamburini A, Daffocchio D (2008) Detection of feed-derived maize DNA in goat milk and evaluation of the potential of horizontal transfer to bacteria. Eur Food Res Technol 277:1699–1709
Rossen L, Norskov P, Holmstrom K, Rasmussen OF (1992) Inhibition of PCR by components of food samples, microbial diagnostic assays and DNA-extraction solutions. Int J Food Microbiol 17:37–45
Terzi V, Infascelli F, Tudisco R, Russo G, Stanca AM, Faccioli P (2004) Quantitative detection of Secale cereal by real time PCR amplification. Lebensmittel-Wissenschaft und – Technologie (LWT) 37:239–246
Tudisco R, Mastellone V, Cutrignelli MI, Lombardi P, Bovera F, Mirabella N, Piccolo G, Calabrò S, Avallone L, Infascelli F (2010) Fate of transgenic DNA and evaluation of metabolic effects in goats fed genetically modified soybean and in their offsprings. Animal 4:1662–1671
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Tudisco, R., Calabrò, S., Cutrignelli, M.I., Grossi, M., Piccolo, V., Infascelli, F. (2012). Influence of Sample Storage on the Quality of DNA Extracted from Milk of Goats Fed Conventional or Transgenic Soybean. In: Pugliese, A., Gaiti, A., Boiti, C. (eds) Veterinary Science. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-23271-8_33
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DOI: https://doi.org/10.1007/978-3-642-23271-8_33
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