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Proteomics of Plant Hyperaccumulators

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Metal Toxicity in Plants: Perception, Signaling and Remediation

Abstract

Plant metal hyperaccumulators take up and detoxify high concentrations of metal ions in their roots and shoots. They constitute an exceptional biological material for understanding mechanisms regulating plant metal homeostasis and plant adaptation to extreme environments. Hyperaccumulation physiology has recently also been studied with molecular tools. Indeed making use of transcriptome analysis it has been demonstrated that different expression patterns of genes accompanied different responses to metals between hyperaccumulator and non-hyperaccumulator plant species. The proteomic approach can also be powerful in dissecting the hyperaccumulator phenotype and the complex involvement of the protein regulation in this phenomenon. This chapter focuses on the recent developments in the application of proteomics to the analysis of hyperaccumulators providing a comprehensive review of key literature data of plant−metal − in particular Cd, Ni and Zn − hyperaccumulation.

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Abbreviations

2D-LC:

Two-dimensional liquid chromatography

2D-PAGE:

Two-dimensional polyacrylamide gel electrophoresis

APX:

Ascorbate peroxidase

ESI:

Electrospray ionization tandem mass spectrometry

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

GST:

Glutathione S transferase

HS:

Heat-shock

IEF:

Isoelectric focusing

iTRAQs:

Isobaric Tag for Relative and Absolute Quantification

LC:

La Calamine

LE:

Lenninger

MALDI-TOF:

Matrix-assisted laser desorption/ionization time-of-flight

MP:

Monte Prinzera

MS:

Mass spectrometry

MudPIT:

Multidimensional protein identification technology

ROS:

Reactive oxygen species

RpR:

(Rožžnov pod Radhštěm-Moravia)

RuBisCo:

Ribulose 1,5-bisphosphate carboxylase/oxybenase

SOD:

Superoxide dismutase

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  1. Department of Environmental Sciences, University of Parma, Parma, Italy

    Giovanna Visioli & Nelson Marmiroli

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  1. Giovanna Visioli
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  2. Nelson Marmiroli
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Correspondence to Nelson Marmiroli .

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  1. CSIC, Bioquímica, Estación Experimental Del Zaidín, Apartado 419, Granada, E-18008, Spain

    Dharmendra K. Gupta

  2. CSIC, Bioquímica, Estación Experimental Del Zaidín, Apartado 419, Granada, E-18080, Spain

    Luisa M. Sandalio

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Visioli, G., Marmiroli, N. (2012). Proteomics of Plant Hyperaccumulators. In: Gupta, D., Sandalio, L. (eds) Metal Toxicity in Plants: Perception, Signaling and Remediation. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-22081-4_8

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