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Applications of Confocal Microscopy in the Study of Root Apparatus

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Measuring Roots

Abstract

Confocal microscopy can be considered as one of the most important progress in optical microscopy within the last decades and has become a powerful investigation tool for molecular, cellular, and development biologist. In this chapter, the authors analyze the main uses of confocal microscopy and fluorescent dyes for studying many aspects of root physiology. The developments of confocal laser scanning microscopy and fluorescent probes that can be applied, in vivo, to plant root cells, have improved new possibilities for imaging cellular components and activities. Moreover, the opportunity to create transgenic plants and cells permits the visualization of fluorescently labeled components in cells, with minimum invasive manipulation. The combination of all this techniques provides more information for the comprehension of physiological and developmental processes in plant roots. Finally, the use of confocal laser microscopy in these types of studies has several advantages: (1) it is simple, rapid, and accurate; (2) it is nonhazardous; (3) a proper use of all the equipment is helpful to not obtain artifacted images but reliable ones.

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Authors and Affiliations

  1. Department of Plant, Soil and Environment, University of Florence, viale delle Idee 30, 50019, Sesto Fiorentino, FI, Italy

    Susanna Pollastri, Elisa Azzarello, Elisa Masi, Camilla Pandolfi & Sergio Mugnai

  2. Dpt. Plant, Soil & Environment, University of Firenze, Viale delle idee 30, Sesto Fiorentino, FI, Italy

    Stefano Mancuso

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  1. Susanna Pollastri
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  2. Elisa Azzarello
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  3. Elisa Masi
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  4. Camilla Pandolfi
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  5. Sergio Mugnai
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  6. Stefano Mancuso
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  1. Dipto. Ortoflorofrutticoltura, Università Firenze, Viale delle Idee 30, Sesto Fiorentino, 50019, Italy

    Stefano Mancuso

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Pollastri, S., Azzarello, E., Masi, E., Pandolfi, C., Mugnai, S., Mancuso, S. (2012). Applications of Confocal Microscopy in the Study of Root Apparatus. In: Mancuso, S. (eds) Measuring Roots. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-22067-8_6

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