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IEF Technique to Study the β-Glucosidase-Humic Complexes in Organic and Mineral Amended Soils

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Soil Enzymology in the Recycling of Organic Wastes and Environmental Restoration

Part of the book series: Environmental Science and Engineering ((ENVSCIENCE))

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Abstract

In this work, fields with different slopes (0, 2 and 6%) located in an erodible area due to topographic and climatic conditions and improper management, were submitted to rehabilitation practices that included almond tree cultivation under organic or mineral fertilization. Conventional parameters usually related to soil fertility and quality (total organic carbon, total nitrogen, water soluble carbon, humic carbon, etc.) along with unconventional parameters (β-glucosidase-humic complexes, soil enzyme activity) were measured with the aim of evaluating the capability of the rehabilitation practices to improve soil quality and/or prevent soil degradation. The application of both organic and mineral fertilizers and the presence of almond trees resulted effective in increasing general soil chemical properties and in particular biochemical properties related to the humic-bound β-glucosidase enzyme (HEG). The analytical isoelectric focussing technique (IEF), was able to isolate and characterise extracellular humic-enzyme complexes discriminating among different slopes and treatments. The organic and mineral fertilisers and plants were more efficient in increasing amount and activity of humic-enzyme complexes in 0 and 2% slopes, while 6% slope showed a significative increase only with organic amendment. The active humic-β-glucosidase enzyme complexes isolated and purified through analytical IEF, even though they represent only a small part of soil humic substances are relevant indicators for assessing amount and role of humic carbon stored in degraded soils, following regeneration practices.

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Authors and Affiliations

  1. Istituto per lo Studio degli Ecosistemi, CNR, Via Moruzzi 1, 56124, Pisa, Italy

    Serena Doni, Cristina Macci, Grazia Masciandaro & Brunello Ceccanti

Authors
  1. Serena Doni
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  2. Cristina Macci
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  3. Grazia Masciandaro
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  4. Brunello Ceccanti
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Corresponding author

Correspondence to Serena Doni .

Editor information

Editors and Affiliations

  1. Departamento de Bioquimica del Suelo, Investigac. Agrobiol. de, Universidade de Santiago de Compostela, Apartado 122, Santiago de Compostela, 15780, Spain

    Carmen Trasar-Cepeda

  2. , Department of Soil and Water Conservatio, CEBAS-CSIC, Espinardo, Spain

    Teresa Hernández

  3. , Department of Soil and Water Conservatio, CEBAS-CSIC, Espinardo, Spain

    Carlos García

  4. Fac. Ciencias, Depto. Microbiología, Universidad de Burgos, Plaza Misael Bañuelos s/n, Burgos, 09001, Spain

    Carlos Rad

  5. , Soil Science and Agricultural Chemistry, University of Burgos, Cantabria Av., Burgos, 09006, Spain

    Salvador González-Carcedo

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Doni, S., Macci, C., Masciandaro, G., Ceccanti, B. (2011). IEF Technique to Study the β-Glucosidase-Humic Complexes in Organic and Mineral Amended Soils. In: Trasar-Cepeda, C., Hernández, T., García, C., Rad, C., González-Carcedo, S. (eds) Soil Enzymology in the Recycling of Organic Wastes and Environmental Restoration. Environmental Science and Engineering(). Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-21162-1_3

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