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Image Analysis for Calculation of the Toxicity Degree of Cells in Phase Contrast Microscopy Images

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Bildverarbeitung für die Medizin 2011

Part of the book series: Informatik aktuell ((INFORMAT))

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Abstract

Because of the very special type of contrast in phase-contrast images, it is almost impossible to perform fully automated single-cell analysis and quantification successfully. Because fluorescent dyes are highly toxic, phase-contrast images are commonly used to monitor live cells. In this paper, we present a method for the fully automated segmentation, classification and quantification of individual cell morphology in phase-contrast images. We calculate the confluence of the cell population and quantify the degree of toxic damage to each individual cell following phenol incubation. The results are then compared to standard cytotoxicity assays.

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References

  1. Debeir O, Adanja I, Warzee N, et al. Phase contrast image segmentation by weak watershed transform assembly. Proc IEEE ISBI. 2008; p. 724–7.

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  3. Athelogou M, Schönmeyer R, Schmidt G, et al. Bildanalyse in Medizin und Biologie Beispiele und Anwendungen, Medizintechnik. In: Life Science Engineering. Springer; 2008. p. 983–1005.

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Author information

Authors and Affiliations

  1. Definiens AG, Munich, Munich, Germany

    M. Athelogou, G. Schmidt & G. Binnig

  2. Institute for Medical Engineering, Technical University Munich, Munich, Germany

    M. Eblenkamp, F. Novotny & E. Wintermantel

Authors
  1. M. Athelogou
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  2. M. Eblenkamp
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  3. G. Schmidt
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  4. F. Novotny
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  5. E. Wintermantel
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  6. G. Binnig
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Corresponding author

Correspondence to M. Athelogou .

Editor information

Editors and Affiliations

  1. , Institut für Medizinische Informatik, Universität zu Lübeck, Ratzeburger Allee 160, Lübeck, 23562, Germany

    Heinz Handels

  2. , Institut für Medizinische Informatik, Universität Lübeck, Ratzeburger Allee 160, Lübeck, 23538, Germany

    Jan Ehrhardt

  3. Inst. Medizinische Informatik, RWTH Aachen, Pauwelsstr. 30, Aachen, 52074, Germany

    Thomas M. Deserno

  4. Abt. Med./Biol.Inform./H0100, Deutsches Krebsforschungzentrum (DKFZ), Im Neuenheimer Feld 280, Heidelberg, 69120, Germany

    Hans-Peter Meinzer

  5. Charité Campus Benjamin Franklin, Inst. Medizinische Informatik,, Universitätsklinikum Berlin, Hindenburgdamm 30, Berlin, 12200, Berlin, Germany

    Thomas Tolxdorff

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© 2011 Springer-Verlag Berlin Heidelberg

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Athelogou, M., Eblenkamp, M., Schmidt, G., Novotny, F., Wintermantel, E., Binnig, G. (2011). Image Analysis for Calculation of the Toxicity Degree of Cells in Phase Contrast Microscopy Images. In: Handels, H., Ehrhardt, J., Deserno, T., Meinzer, HP., Tolxdorff, T. (eds) Bildverarbeitung für die Medizin 2011. Informatik aktuell. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-19335-4_29

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