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Understanding Vegetative Desiccation Tolerance Using Integrated Functional Genomics Approaches Within a Comparative Evolutionary Framework

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Plant Desiccation Tolerance

Part of the book series: Ecological Studies ((ECOLSTUD,volume 215))

Abstract

Desiccation tolerance (DT) is defined as the equilibration of protoplasmic water potential with that of the surrounding air (generally dry) without loss of viability upon rehydration. Vegetative DT is widespread among mosses and lichens, but is relatively rare in vascular plants (0.15%). Recent studies of selected resurrection species indicate that while resurrection plants might have evolved unique adaptive proteins, enzymes, and antioxidants, the molecular genetic basis of DT lies in the orchestration of transcriptional and posttranscriptional regulatory programs that operate during drying and rehydration. DT requires signaling pathways and regulatory mechanisms, aspects of which resemble developmental programs present in orthodox seeds, which result in the accumulation of oligosaccharides, stress adaptive proteins, antioxidants, reactive oxygen scavenging enzymes, as well as alterations in the composition and structure of membrane lipids. Functional genomics studies using transcriptome, proteome, and metabolome analyses are just beginning to unravel the system complexity required to orchestrate the metabolic symphony that is DT. The status of current gene discovery efforts is summarized along with major transcriptome technologies available currently to conduct desiccation sensitive versus tolerant species comparisons. These strategies, integrated with large-scale proteomic and metabolomic investigations currently in progress, promise to revolutionize our understanding of the mechanistic basis of desiccation tolerance in resurrection plants.

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Acknowledgments

This work was supported by grants from the United States Department of Agriculture (USDA) National Research Initiative (NRI) (CREES-NRI-2007-02007) to MJ and JCC, and the University of Nevada Agricultural Experiment Station. Support for the Nevada Proteomics Center was made possible by NIH Grant Number P20 RR-016464 from the INBRE-BRIN Program of the National Center for Research Resources and the NIH IDeA Network of Biomedical Research Excellence (INBRE, RR-03-008).

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  1. Department of Biochemistry and Molecular Biology, MS200, University of Nevada, Reno, NV, 89557-0200, USA

    John C. Cushman

  2. USDA-ARS Plant Genetics Research Unit, 205 Curtis Hall, University of Missouri, Columbia, MO, 65211, USA

    Melvin J. Oliver

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Correspondence to John C. Cushman .

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  1. Inst. Botanik, TU Darmstadt, Schnittspahnstr. 3-5, Darmstadt, 64287, Germany

    Ulrich Lüttge

  2. Inst. Pflanzenphysiologie, Univ. Bayreuth, Bayreuth, 95440, Germany

    Erwin Beck

  3. Inst. Molekulare Physiologie und, Biotechnologie der Pflanzen (IMBIO), Universität Bonn, Kirschallee 1, Bonn, 53115, Germany

    Dorothea Bartels

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Cushman, J.C., Oliver, M.J. (2011). Understanding Vegetative Desiccation Tolerance Using Integrated Functional Genomics Approaches Within a Comparative Evolutionary Framework. In: Lüttge, U., Beck, E., Bartels, D. (eds) Plant Desiccation Tolerance. Ecological Studies, vol 215. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-19106-0_15

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