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Control of DNA Replication by Cyclin-Dependent Kinases in Development

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Cell Cycle in Development

Part of the book series: Results and Problems in Cell Differentiation ((RESULTS))

Abstract

Cyclin-dependent kinases (CDKs) are required for initiation of DNA replication in all eukaryotes, and appear to act at multiple levels to control replication origin firing, depending on the cell type and stage of development. In early development of many animals, both invertebrate and vertebrate, rapid cell cycling is coupled with transcriptional repression, and replication initiates at closely spaced replication origins with little or no sequence specificity. This organisation of DNA replication is modified during development as cell proliferation becomes more controlled and defined. In all eukaryotic cells, CDKs promote conversion of “licensed” pre-replication complexes (pre-RC) to active initiation complexes. In certain circumstances, CDKs may also control pre-RC formation, transcription of replication factor genes, chromatin remodelling, origin spacing, and organisation of replication origin clusters and replication foci within the nucleus. Although CDK1 and CDK2 have overlapping roles, there is a limit to their functional redundancy. Here, I review these findings and their implications for development.

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Acknowledgements

Daniel Fisher is an employee of Inserm. This work benefited from ARC grant 1047, and the laboratory is currently funded by a grant from the Agence Nationale de la Recherche ANR-09-BLAN-0252-01, and from the Ligue Nationale Contre le Cancer, EL2010.LNCC/DF.

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  1. IGMM, CNRS UMR 5535, 1919 Route de Mende, 34293, Montpellier, France

    Daniel Fisher

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  1. CNRS UMR 6061, Fac. Médecine, Université de Rennes 1, Rennes CX, 35043, France

    Jacek Z. Kubiak

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Fisher, D. (2011). Control of DNA Replication by Cyclin-Dependent Kinases in Development. In: Kubiak, J. (eds) Cell Cycle in Development. Results and Problems in Cell Differentiation. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-19065-0_10

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