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Experience from Clinical Trials with Fertility Control Vaccines

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Immune Infertility

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Abstract

Three vaccines for hCG have undergone Phase I Clinical trial but only one HSD-hCG vaccine went through Phase II efficacy trials demonstrating its ability to prevent pregnancy without impairment of ovulation and derangement of menstrual cycles and bleeding profiles. One semi-synthetic vaccine against LHRH has had Phase I/II clinical trials in India and Austria in 28 patients of carcinoma prostate. In this chapter, highlights of these vaccines are presented and issues related to clinical trials are discussed.

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References

  1. Fishel SB, Edwards RG, Evans CJ (1984) Human chorionic gonadotropin secreted by preimplantation embryos cultured in vitro. Science 223(4638):816–818

    Article  CAS  PubMed  Google Scholar 

  2. Hearn JP, Gidley-Baird AA, Hodges JK (1988) Embryonic signals during the preimplantation period in primates. J Reprod Fertil Suppl 36:49–58

    CAS  PubMed  Google Scholar 

  3. Talwar GP, Singh O, Pal R et al (1994) A vaccine that prevents pregnancy in women. Proc Natl Acad Sci U S A 91:8532–8536

    Article  CAS  PubMed  Google Scholar 

  4. Talwar GP, Singh O, Gupta SK et al (1997) The HSD-hCG vaccine prevents pregnancy in women, fesability studies of reversibily safe contraception vaccine. Am J Reprod Immunol 37:153–160

    CAS  PubMed  Google Scholar 

  5. Dhar R, Karmakar S, Sriraman R et al (2004) Efficacy of a recombinant chimeric anti-hCG antibody to prevent human cytotrophoblasts fusion and block progesterone synthesis. Am J Reprod Immunol 51:358–363

    Article  PubMed  Google Scholar 

  6. Ramakrishnan S, Das C, Dubey SK (1979) Immunogenecity of three C-terminal synthetic peptides of the beta subunit of human chorionic gonadotropin and properties of the antibodies raised against 45-aminoacid C-terminal peptide. J Reprod Immunol 1:249–261

    Article  CAS  PubMed  Google Scholar 

  7. Sahal D, Ramakrishnan S, Iyer KSN et al (1982) Immunobiological properties of a carboxy-terminal 53-amino acid peptide of the beta subunit of human chorionic gonadotropin. J Reprod Immunol 4:145–146

    Article  CAS  PubMed  Google Scholar 

  8. Nash H, Talwar GP, Segal S et al (1980) Observation on the antigenecity and clinical effects of a candidate antipregnancy vaccine: B-subunit of human chorionic gonadotropin linked to tetanus toxoid. Fertil Steril 34:328–335

    CAS  PubMed  Google Scholar 

  9. Thau RB, Witkin SS, Bond MG et al (1983) Effects of long-term immunization against the beta-subunit of ovine luteinizing hormone on circulating immune complex formation and on arterial changes in rhesus monkeys. Am J Reprod Immunol 3:83–88

    CAS  PubMed  Google Scholar 

  10. Thau RB, Bond MG, Witkins SS (1986) Lack of toxicological effects following seven years of active immunization of rhesus monkeys with the beta subunit of ovine leutinizing hormone. In: Talwar GP (ed) Immunological approaches to contraception and promotion of fertility. Plenum, New York, pp 25–33

    Google Scholar 

  11. Deshmukh US, Talwar GP, Gupta SK (1994) Antibody response against three epitopic domains on human chorionic gonadotropins (hCG) in women and rodents immunized with a hCGβ-based immunocontraceptive vaccine. J Clin Immunol 14:162–168

    Article  CAS  PubMed  Google Scholar 

  12. Rose NR, Burek CL, Smith JP (1988) Safety evaluation of hCG vaccine in primates: auto-antibodies production. In: Talwar GP (ed) Contraception research for today and nineties. Springer, New York, pp 231–239

    Google Scholar 

  13. Jones WR, Bradley J, Judd SJ et al (1988) Phase I clinical trial of a World Health Organization birth control vaccine. Lancet 1:1295–1298

    Article  CAS  PubMed  Google Scholar 

  14. Talwar GP, Sharma NC, Dubey SK et al (1976) Isoimmunisation against human chorionic gonadotropin with conjugates of processed beta subunit of the hormone and tetanus toxoid. Proc Natl Acad Sci U S A 73:218–222

    Article  CAS  PubMed  Google Scholar 

  15. Gaur A, Arunan K, Singh O, Talwar GP (1990) Bypass by an alternate carrier of acquired unresponsiveness to hCG upon repeated immunization with tetanus conjugated vaccine. Int Immunol 2:151–155

    Article  CAS  PubMed  Google Scholar 

  16. Szturmowicz M, Slodkowska J, Zych J et al (1999) Frequency and clinical significance of beta-subunit human chorionic gonadotropin expression in non- small cell lung cancer patients. Tumour Biol 20:99–104

    Article  CAS  PubMed  Google Scholar 

  17. Iles RK, Persad R, Trivedi M (1996) Urinary concentration of human chorionic gonadotropin and its fragments as a prognostic marker in bladder cancer. Br J Urol 77:61–69

    Article  CAS  PubMed  Google Scholar 

  18. Lundin M, Nordling S, Carpelan-Holmstrom M (2000) A comparison of serum and tissue hCG beta as prognostic markers in colorectal cancer. Anticancer Res 20:4949–4951

    CAS  PubMed  Google Scholar 

  19. Rau B, Below C, Liebrich W (1995) Significance of serum hCGβ as a tumor marker for stomach carcinoma. Langenbecks Arch Chir 380:359–364

    Article  CAS  PubMed  Google Scholar 

  20. Alfthan H, Haglund C, Roberts P et al (1992) Elevation of free beta subunit of human chorionic gonadotropin and core beta fragment of human chorionic gonadotropin in the serum and urine of patients with malignant pancreatic and biliary diseases. Cancer Res 52:4628–4633

    CAS  PubMed  Google Scholar 

  21. Bièche I, Lazar V, Noguès C et al (1998) Prognostic value of chorionic gonadotropin beta gene transcripts in human breast carcinoma. Clin Cancer Res 4:671–676

    PubMed  Google Scholar 

  22. Crawford RA, Iles RK, Carter PG et al (1998) The prognostic significance of beta human chorionic gonadotropin and its metabolites in women with cervical carcinoma. J Clin Pathol 51:685–688

    Article  CAS  PubMed  Google Scholar 

  23. Bhalang K, Kafrawy AH, Miles DA (1999) Immunohistochemical study of the expression of human chorionic gonadotropin-beta in oral squamous cell carcinoma. Cancer 85:757–762

    Article  CAS  PubMed  Google Scholar 

  24. Scholl PD, Jurco S, Austin JR (1997) Ectopic production of beta-HCG by a maxillary squamous cell carcinoma. Head Neck 19:701–705

    Article  CAS  PubMed  Google Scholar 

  25. Carter PG, Iles RK, Neven P et al (1995) Measurement of urinary beta core fragment of human chorionic gonadotropin in women with vulvovaginal malignancy and its prognostic significance. Br J Cancer 71:350–353

    CAS  PubMed  Google Scholar 

  26. De Bruijn HW, Ten Hoor KA, Krans M et al (1997) Rising serum values of beta-subunit human chorionic gonadotropin (hCG) in patients with progressive vulvar carcinomas. Br J Cancer 75:1217–1218

    PubMed  Google Scholar 

  27. Sheaff MT, Martin JE, Badenoch DF et al (1996) Beta hCG as a prognostic marker in adenocarcinoma of the prostate. J Clin Pathol 49:693–694

    Article  Google Scholar 

  28. Hotakainen K, Ljungberg B, Paju A (2002) The free beta-subunit of human chorionic gonadotropin as a prognostic factor in renal cell carcinoma. Br J Cancer 86:185–189

    Article  CAS  PubMed  Google Scholar 

  29. Kathuria S, Nath R, Pal R et al (2002) Functional recombinant antibodies against hCG expressed in plants. Curr Sci 82:1452–1457

    CAS  Google Scholar 

  30. Kathuria S, Sriraman R, Nath R et al (2002) Efficacy of plant produced recombinant antibodies against hCG. Hum Reprod 17:2054–2061

    Article  CAS  PubMed  Google Scholar 

  31. Kabeer RS, Pal R, Talwar GP (2005) Human acute lymphoblastic leukemia cells make human pregnancy hormone hCG and expose it on the membrane: a case for using recombinant antibody against hCG for selective delivery of drugs and/or radiations. Curr Sci 89:1571–1576

    CAS  Google Scholar 

  32. Talwar GP, Chaudhuri MK, Jayshankar R (1992) Inventors. Antigenic derivative of GnRH. UK Patent, 2228262

    Google Scholar 

  33. Jayashankar R, Chaudhari M, Singh OM (1989) Semisynthetic anti-LHRH vaccine causing atrophy of the prostate. Prostate 14:3–11

    Article  CAS  PubMed  Google Scholar 

  34. Giri DK, Jayaraman S, Neelaram GS (1991) Prostatic hypoplasia in Bonnet monkeys following active immunization with semi-synthetic anti-LHRH vaccine. Exp Mol Pathol 54:255–264

    Article  CAS  PubMed  Google Scholar 

  35. Rovan E, Fiebiger E, Kalla NR et al (1992) Effect of active immunization to luteinizing-­hormone-releasing hormone on the fertility and histoarchitecture of the reproductive organs of male rat. Urol Res 20:323–334

    Article  CAS  PubMed  Google Scholar 

  36. Shastri N, Manhar SK, Talwar GP (1981) Important role of the carrier in the induction of antibody response without Freund’s complete adjuvant against a “self” peptide hormone LHRH. Am J Reprod Immunol 1:262–265

    CAS  Google Scholar 

  37. Fuerst J, Fiebiger E, Jungwirth A (1997) Effect of active immunization against luteinizing hormone-releasing hormone on the androgen-sensitive Dunning R3327-PAP and androgen independent Dunning R3327-AT2.1 prostate cancer sublines. Prostate 32:77–84

    Article  CAS  PubMed  Google Scholar 

  38. Diwan M, Dawar H, Talwar GP (1998) Induction of early and bioeffective antibody response in rodents with the anti LHRH vaccine given as a single dose in biodegradable microspheres along with alum. Prostate 35:279–284

    Article  CAS  PubMed  Google Scholar 

  39. Gupta JC, Raina K, Talwar GP (2004) Engineering, cloning and expression of genes encoding the multimeric luteinising- hormone-releasing-hormone linked to T-cell determinants in Escherichia coli. Protein Expr Purif 37:1–7

    Article  CAS  PubMed  Google Scholar 

  40. Raina K, Panda AK, Ali MM (2004) Purification, refolding, and characterization of recombinant LHRH-T multimer. Protein Expr Purif 37:8–17

    Article  CAS  PubMed  Google Scholar 

  41. Talwar GP, Raina K, Gupta JC et al (2004) A recombinant luteinising-hormone-releasing-hormone immunogen bioeffective in causing prostatic atrophy. Vaccine 22:3713–3721

    Article  CAS  PubMed  Google Scholar 

  42. Miller LA, Talwar GP, Killlian GJ (2006) Contraceptive effect of a recombinant GnRH vaccine in adult female pigs. Proc Vertebr Pest Conf 22:106–109

    Google Scholar 

  43. Vyas HK, Pal R, Vishwakarma R et al (2009) Selective killing of leukemia and lymphoma cells expressing ectopically hCGb by a conjugate of curcumin with an antibody against hCGb subunit. Oncology 76:101–111

    Article  CAS  PubMed  Google Scholar 

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Acknowledgment

Our current work on the hCG vaccine is supported by Grant from the Dept. of Biotechnology, Govt. of India.

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Authors and Affiliations

  1. Talwar Research Foundation, E-8 Neb Valley, Neb Sarai, 110068, New Delhi, India

    G. P. Talwar

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  1. G. P. Talwar
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  2. Shilpi Purswani
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  3. Jagdish Chandra Gupta
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  4. Hemant Kumar Vyas
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Corresponding author

Correspondence to G. P. Talwar .

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Editors and Affiliations

  1. Klinik für Andrologie und Venerologie, Universitätsklinikum Marburg, Deutschhausstr. 9, Marburg, 35033, Germany

    Walter K.H. Krause

  2. School of Medicine, West Virginia University, Morgantown, 26506, U.S.A.

    Rajesh K. Naz

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© 2009 Springer-Verlag Berlin Heidelberg

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Talwar, G.P., Purswani, S., Gupta, J.C., Vyas, H.K. (2009). Experience from Clinical Trials with Fertility Control Vaccines. In: Krause, W., Naz, R. (eds) Immune Infertility. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-01379-9_19

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  • DOI: https://doi.org/10.1007/978-3-642-01379-9_19

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-01378-2

  • Online ISBN: 978-3-642-01379-9

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