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In Vitro Compartmented Systems to Study Transport in Arbuscular Mycorrhizal Symbiosis

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Symbiotic Fungi

Part of the book series: Soil Biology ((SOILBIOL,volume 18))

Abstract

With the development of compartmented systems allowing the separation of the mycorrhizal roots from the extraradical mycelium, studies on arbuscular mycorrhizal (AM) fungal transport have been performed since the middle of the 1970s. They rapidly led to the demonstration that AM fungi can transport nutrients to their hosts. While soil-based systems were initially used and continue to be widely employed, AM fungal transport is also commonly studied with in vitro culture systems, as they offer distinctive advantages.

This chapter aims at presenting the techniques and methodology for performing AM fungal transport studies, using compartment root-organ cultures but also the two newly developed systems in which AM fungi can be associated with autotrophic plants under strict in vitro conditions.

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Acknowledgements

H.D.D.B. acknowledges receipt of a FRIA fellowship via the FNRS (Belgium) and a grant of Chargé de cherches from the F.R.S – FNRS (Belgium). L.V. was supported by a grant of the “Fonds Spéciaux de Recherche” (FSR) of the Université Catholique de Louvain.

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Authors and Affiliations

  1. Unité de Microbiologie, Université catholique de Louvain, Croix du Sud 3, 1348, Louvain-la-Neuve, Belgium

    H. Dupré de Boulois, L. Voets & S. Declerck

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  1. H. Dupré de Boulois
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  2. L. Voets
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  3. S. Declerck
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Correspondence to H. Dupré de Boulois .

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Editors and Affiliations

  1. Amity Institute of Microbial Sciences, Amity University Uttar Pradesh, Noida, 201303, India

    Ajit Varma  & Amit C. Kharkwal  & 

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de Boulois, H.D., Voets, L., Declerck, S. (2009). In Vitro Compartmented Systems to Study Transport in Arbuscular Mycorrhizal Symbiosis. In: Varma, A., Kharkwal, A.C. (eds) Symbiotic Fungi. Soil Biology, vol 18. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-95894-9_7

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